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Immunocytochemistry/ Immunofluorescence - Membrin antibody [4HAD6 ] (ab13511)
![Immunocytochemistry/ Immunofluorescence - Membrin antibody [4HAD6 ] (ab13511)](/ps/datasheet/images/ab13511_1.jpg)
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Product Name
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Membrin antibody [4HAD6 ]
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See all Membrin antibodies (4)...
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Product type
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Primary antibodies
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Description
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Mouse monoclonal [4HAD6 ] to Membrin
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Immunogen
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Recombinant rat membrin with a His6 tag
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Reacts with
(species key)
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Hu, Ms, Rat, Chk, Dog, Hm, Pig, Rb
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Tested applications
(see key)
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ICC/IF, IP, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ICC: 1/500.
ICC/IF: 1/500 (see Abreview)
IP: Use at a concentration of 2 - 4 µg/ml.
WB: Use at a concentration of 2 µg/ml. This concentration detects membrin in 20µg of HeLa heat shocked cell lysate by ECL analysis. Predicted molecular weight: 27 kDa.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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HeLa heat shocked lysate
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Cellular localization
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Golgi Apparatus
Type IV membrane protein
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Research areas
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Signal Transduction >> Protein Trafficking >> Vesicle Transport >> SNAPs & SNAREs
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Relevance
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Membrin is a 27 kDa integral membrane protein on the surface of the Golgi apparatus that serves as a tSNARE in ER to Golgi transport. GS27 is a member of a protein complex that consists of cis-Golgi vesicle receptor syntaxin 5 and GS28, vSNARE rbet1 and rsec22, and rsly1. Immunoprecipitation analysis has shown that there are two subcomplexes within this complex: one contains syntaxin 5 and GOS28 and the other one contains syntaxin 5, GS27/membrin, rsec22 and rbet1. These complexes are postulated to direct the fusion of ER-derived vesicles with Vesicular tubular cluster (VTC), and the fusion of VTCs to form cis-Golgi compartment.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Mouse
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Clonality
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Monoclonal
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Clone number
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4HAD6
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Isotype
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IgG1
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Purity
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Protein G purified
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Storage buffer
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Preservative: None
Constituents: 50% Glycerol, 0.1mM PMSF, PBS, pH 7.2 Material safety datasheet (MSDS) for this product:
Glycerol MSDS
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Form
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Liquid
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Concentration
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1.000 mg/ml
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this Membrin antibody [4HAD6 ] is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for Membrin antibody [4HAD6 ] images, references, products related to ab13511 and other tools.
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Membrin antibody [4HAD6 ] images:
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 Immunocytochemistry/ Immunofluorescence - Membrin antibody [4HAD6 ] (ab13511)
ab13511 at 1/500 dilution staining human HeLa cells by ICC/IF. HeLa cells, grown on coverslips, were fixed in 3% paraformaldehyde in PBS for 15 minutes. After washing with PBS, the reaction was quenched with 50 mM ammonium chloride for 10 mins. The cells were permeabilized using 0.1 % Triton X100 in PBS, for 2 minutes. After washing 2 times with PBS, the sample was blocked with 2% fish gelatine in PBS. for 1 hour. ab13511 was diluted in the blocking solution, put on the coverslips and incubated for 1 hour on room temperature. After washing 3-4 times with the blocking solution, the sample was incubated in the secondary antibody (Alexa Fluor ® 488 conjugated Goat Anti-Mouse IgG (H+L) diluted in the blocking solution), for 1 hour at room temeperature. The coverslips were washed 6 times with the blocking solution and then mounted on microscope slides. This image is courtesy of an Abreview submitted by Mr Denis Krndija
See Abreview
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References for Membrin antibody [4HAD6 ] (ab13511)
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This product has been used in:
Flowerdew SE & Burgoyne RD A VAMP7/Vti1a SNARE complex distinguishes a non-conventional traffic route to the cell surface used by KChIP1 and Kv4 potassium channels. Biochem J 418:529-40 (2009). ICC/IF; Human. PubMed: 19138172
If you publish research using ab13511 please let us know so that we can cite the reference on this datasheet.
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Search PubMed (MEDLINE) for references to Membrin
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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