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Anti-Menin antibody - ChIP Grade (ab2605)

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Overview

Product name

Anti-Menin antibody - ChIP Grade
See all Menin products (12) ...

Description

Rabbit polyclonal to Menin - ChIP Grade

Tested applications

ChIP, IHC-P, ICC/IF, IP, WBmore details

Cross reactivity

Reacts with

Mouse, Human

Predicted to work with

Rat

Immunogen

Synthetic peptide (Human) - which represents a portion of the C-terminus of human MEN1.

Positive control

293T cell lysate.

Properties

Form

Liquid

Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Storage buffer

Preservative: 0.1% Sodium Azide
Constituents: 8mM PBS, 60mM Citrate, 150mM Tris, pH 7-8

Concentration

Concentration information loading...

Purification notes

Affinity purified using the immunising peptideimmobilized on solid support.

Clonality

Polyclonal

Isotype

IgG

Research areas

Epigenetics and Nuclear Signaling >> ChIP'ing antibodies >> ChIP'ing antibodies
Epigenetics and Nuclear Signaling >> Transcription >> Cancer susceptibility >> Tumor Suppressors

  • Western blot - Menin antibody - ChIP Grade (ab2605)Western blot - Menin antibody - ChIP Grade (ab2605) image (enlarge)

  • Immunocytochemistry/ Immunofluorescence - Menin antibody (ab2605)Immunocytochemistry/ Immunofluorescence - Menin antibody (ab2605) image (enlarge)

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Menin antibody - ChIP Grade(ab2605)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Menin antibody - ChIP Grade(ab2605) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab2605 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application notes

ChIP: Use at a concentration of 1 - 4 µg/ml.
ICC/IF: 1/300 - 1/600.
IHC-P: 1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
IP: Use at 2µg/mg of lysate.
WB: 1/1000 - 1/10000. Detects a band of approximately 68 kDa (predicted molecular weight: 68 kDa). Can be blocked with Menin peptide (ab34783).


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Target

Function

Essential component of a MLL/SET1 histone methyltransferase (HMT) complex, a complex that specifically methylates 'Lys-4' of histone H3 (H3K4). Functions as a transcriptional regulator. Binds to the TERT promoter and represses telomerase expression. Plays a role in TGFB1-mediated inhibition of cell-proliferation, possibly regulating SMAD3 transcriptional activity. Represses JUND-mediated transcriptional activation on AP1 sites, as well as that mediated by NFKB subunit RELA. Positively regulates HOXC8 and HOXC6 gene expression. May be involved in normal hematopoiesis through the activation of HOXA9 expression (By similarity). May be involved in DNA repair.

Tissue specificity

Ubiquitous.

Involvement in disease

Defects in MEN1 are the cause of familial multiple endocrine neoplasia type I (MEN1) [MIM:131100]. Autosomal dominant disorder characterized by tumors of the parathyroid glands, gastro-intestinal endocrine tissue, the anterior pituitary and other tissues. Cutaneous lesions and nervous-tissue tumors can exist. Prognosis in MEN1 patients is related to hormonal hypersecretion by tumors, such as hypergastrinemia causing severe peptic ulcer disease (Zollinger-Ellison syndrome, ZES), primary hyperparathyroidism, and acute forms of hyperinsulinemia.
Defects in MEN1 are the cause of familial isolated hyperparathyroidism (FIHP) [MIM:145000]; also known as hyperparathyroidism type 1 (HRPT1). FIHP is an autosomal dominant disorder characterized by hypercalcemia, elevated parathyroid hormone (PTH) levels, and uniglandular or multiglandular parathyroid tumors.

Post-translational
modifications

Phosphorylated upon DNA damage, probably by ATM or ATR.

Cellular localization

Nucleus. Concentrated in nuclear body-like structures. Relocates to the nuclear matrix upon gamma irradiation.

Target information above from: UniProt accessionO00255 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • MEA 1 antibody
  • MEA1 antibody
  • MEN 1 antibody
  • MEN1 antibody
  • MEN1_HUMAN antibody
  • Menin antibody
  • Multiple Endocrine Adenomatosis 1 antibody
  • Multiple Endocrine Neoplasia 1 antibody
  • SCG 2 antibody
  • SCG2 antibody
  • Suppressor Candidate Gene 2 antibody
  • Wermer syndrome antibody
  • ZES antibody
  • Zollinger Ellison Syndrome antibody
see all

Database links

see all

Anti-Menin antibody - ChIP Grade images:

  Western blot - Menin antibody - ChIP Grade (ab2605)

Western blot - Menin antibody - ChIP Grade (ab2605)



Predicted band size : 68 kDa

  Immunocytochemistry/ Immunofluorescence - Menin antibody (ab2605)

Immunocytochemistry/ Immunofluorescence - Menin antibody (ab2605)

Detection of menin in 293 cells using ab2605 (2ug/ml). Secondary antibody is Cy3 conjugated anti rabbit IgG.

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Menin antibody - ChIP Grade(ab2605)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Menin antibody - ChIP Grade(ab2605)

Ab2605 staining Human normal placenta. Staining is localized to the nucleus.
Left panel: with primary antibody at 4 ug/ml. Right panel: isotype control.
Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 AR buffer EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS), then incubated with primary antibody for 20 minutes, and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.

References for Anti-Menin antibody - ChIP Grade (ab2605)

This product has been referenced in:

  • Neziri D  et al. Cloning and molecular characterization of Dashurin encoded by C20orf116, a PCI-domain containing protein. Biochim Biophys Acta 1800:430-438 (2010). WB; Human.Read more (PubMed: 20036718) »
  • Liu H  et al. Phosphorylation of MLL by ATR is required for execution of mammalian S-phase checkpoint. Nature 467:343-6 (2010). WB; Human.Read more (PubMed: 20818375) »

See all 6 publications for this product

Publishing research using ab2605? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"