Anti-Mephalan antibody [MP5/73] (ab97892)
Key features and details
- Rat monoclonal [MP5/73] to Mephalan
- Suitable for: ELISA
- Reacts with: Species independent
- Isotype: IgG2b
Overview
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Product name
Anti-Mephalan antibody [MP5/73] -
Description
Rat monoclonal [MP5/73] to Mephalan -
Host species
Rat -
Specificity
This antibody was developed to improve detection of melphalan-DNA adducts through its ability to recognise the stabilised adduct. This antibodiy can be used for quantification of melphalan adducts in purified DNA by ELISA and for immunofluorescent detection of melphalan-DNA adducts in single cells. -
Tested applications
Suitable for: ELISAmore details -
Species reactivity
Reacts with: Species independent -
Immunogen
Full length native protein (purified) corresponding to Mephalan.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
Preservative: 0.02% Sodium azide
Constituent: 99.98% PBS -
Concentration information loading...
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Purity
Protein A/G purified -
Clonality
Monoclonal -
Clone number
MP5/73 -
Myeloma
Y3/Ag1.2.3 -
Isotype
IgG2b -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab97892 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ELISA |
Use at an assay dependent dilution.
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Notes |
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ELISA
Use at an assay dependent dilution. |
Target
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Relevance
Mephalan hydrochloride is a chemotherapy drug belonging to the class of nitrogen mustard alkylating agents.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (3)
ab97892 has been referenced in 3 publications.
- Rothbarth J et al. Immunohistochemical detection of melphalan-DNA adducts in colon cancer cells in vitro and human colorectal liver tumours in vivo. Biochem Pharmacol 67:1771-8 (2004). PubMed: 15081876
- Frank AJ & Tilby MJ Quantification of DNA adducts in individual cells by immunofluorescence: effects of variation in DNA conformation. Exp Cell Res 283:127-34 (2003). PubMed: 12581733
- Tilby MJ et al. Immunological detection of DNA damage caused by melphalan using monoclonal antibodies. Cancer Res 47:1542-6 (1987). PubMed: 3815354