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Mepyramine Fluorescent ligand (Red) (ab118174)

Q&A (1)

Overview

  • Product nameMepyramine Fluorescent ligand (Red)
  • Description

    Fluorescent H1 antagonist

  • Alternative names
    H1-BY633-AN
  • Biological descriptionFluorescent H1 antagonist (pKD values are 8.07 and 5.7 at H1 and H2 respectively). No intrinsic agonist activity seen in vitro. Wide range of applications which include localizing receptor distribution in tissues and cells, and live-cell imaging of receptor kinetics.

    Excitation wavelength: 633 nm

    Emission wavelength: 650 nm

  • Purity> 97%

    • Properties

  • PharmacophoreMepyramine-derivative
  • SelectivityH1: 8.07 | H2: 5.37 | H3: >6
  • Excitation633nm
  • Emission650nm
  • Molecular Weight948.00
  • Molecular formulaC51H60N7O6F2BS
  • Validation notesTo determine the KD for ab118174 at histamine H1 receptors, cells were treated with concentrations of histamine agonist alone, or in the presence of 1 µM ab118174, and the serum-induced expression of SPAP measured.
    The KD at H1 was calculated from the shift of the agonist response curve in the presence of ab118174, compared to the response curve for the agonist alone.
    Similar studies were conducted with the ab118174 ligand using a recombinant CHO cell line expressing the Human H2 receptor and a cyclic AMP-responsive SPAP reporter gene. The KD at H2 was calculated in the same way .
  • Storage instructionsStore at -20°C. Avoid exposure to light.
  • HandlingFor imaging at the H1 receptor use solutions up to 100 nM in DMSO.
  • Research Areas

    • Applications

    Our Abpromise guarantee covers the use of ab118174 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    Fluorescent Cell Imaging Fluorescent Cell Imaging: Use at an assay dependent concentration.
    Small Molecule Fluorescent Optical Detection Small Molecule Fluorescent Optical Detection: Use at an assay dependent concentration.
    Receptor Localization Receptor Localization: Use at an assay dependent concentration.

    Mepyramine Fluorescent ligand (Red) images

    • Receptor Localization - Mepyramine Fluorescent ligand (Red) (ab118174)
      Receptor Localization - Mepyramine Fluorescent ligand (Red) (ab118174)
      Left: ab118174 ligand (100 nM) binding to CHO cells expressing histamine H1 receptors. Center: Binding to CHO-H1 cells blocked by the unlabelled competitor triprolidine (1 µM). Right: No binding of ab118174 to host CHO cells (not expressing H1 receptors). Nuclei have been counter-stained with Hoechst.
    • Small Molecule Fluorescent Optical Detection - Mepyramine Fluorescent ligand (Red) (ab118174)
      Small Molecule Fluorescent Optical Detection - Mepyramine Fluorescent ligand (Red) (ab118174)
      To determine the apparent KD for ab118174 at histamine H1 receptors, cells were treated with varying concentrations of histamine agonist alone, or in the presence of 1 µM ab118174, and the serum-induced expression of SPAP measured. The apparent KD at H1 was calculated from the rightward shift of the agonist response curve in the presence of ab118174, compared to the response curve for the agonist alone. KD value for H1 = 8.07 Antagonist –log KD values
    • Small Molecule Fluorescent Optical Detection - Mepyramine Fluorescent ligand (Red) (ab118174)
      Small Molecule Fluorescent Optical Detection - Mepyramine Fluorescent ligand (Red) (ab118174)
      The KD for ab118174 ligand was assessed using a recombinant CHO cell line expressing the Human H2 receptor and a cyclic AMP-responsive SPAP reporter gene. The apparent KD at H2 was calculated in the same way to that calculated for the Human H1 receptor. The KD value for H2 was found to be 5.37 Antagonist -log KD

    References for Mepyramine Fluorescent ligand (Red) (ab118174)

    ab118174 has not yet been referenced specifically in any publications.

    Publishing research using ab118174 ? Please let us know so that we can cite the reference in this datasheet

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