Overview

  • Product nameAnti-Mesothelin antibody [K1]
    See all Mesothelin primary antibodies
  • Description
    Mouse monoclonal [K1] to Mesothelin
  • Tested applicationsWB, IHC-Fr, Flow Cyt, IHC-Pmore details
  • Species reactivity
    Reacts with: Human, Cynomolgus Monkey
  • Immunogen

    Spleen lymphocytes from mice fused with OVCAR-3, an ovarian tumorcell line derived from poorly differentiated adenocarcinoma of the ovary.

  • Positive control
    • Tonsil, lymph node and OVCAR-3 cells (human).
  • General notes

    This product is sold as crude ascites fluid so we estimate the IgG concentration ~1-3mg/ml.

Properties

Applications

Our Abpromise guarantee covers the use of ab3362 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Detects a band of approximately 73 kDa (predicted molecular weight: 69 kDa). PubMed: 18490724
IHC-Fr Use at an assay dependent concentration.

Acetone fixation was used.

Flow Cyt Use at an assay dependent concentration. PubMed: 18490724
IHC-P Use at an assay dependent concentration. Antigen retrieval is not essential but may optimise staining.

One hour primary antibody incubation was used.

Target

  • FunctionMembrane-anchored forms may play a role in cellular adhesion.
    Megakaryocyte-potentiating factor (MPF) potentiates megakaryocyte colony formation in vitro.
  • Tissue specificityExpressed in lung. Expressed at low levels in heart, placenta and kidney. Expressed in mesothelial cells. Highly expressed in mesotheliomas, ovarian cancers, and some squamous cell carcinomas (at protein level).
  • Involvement in diseaseNote=Antibodies against MSLN are detected in patients with mesothelioma and ovarian cancer.
  • Sequence similaritiesBelongs to the mesothelin family.
  • Post-translational
    modifications
    Both MPF and the cleaved form of mesothelin are N-glycosylated.
    Proteolytically cleaved by a furin-like convertase to generate megakaryocyte-potentiating factor (MPF), and the cleaved form of mesothelin.
  • Cellular localizationSecreted and Cell membrane. Golgi apparatus.
  • Information by UniProt
  • Database links
  • Alternative names
    • CAK 1 antibody
    • CAK1 antibody
    • CAK1 antigen antibody
    • cleaved form antibody
    • Megakaryocyte potentiating factor antibody
    • Mesothelin antibody
    • Mesothelin isoform 1 precursor antibody
    • MPF antibody
    • MSLN antibody
    • MSLN_HUMAN antibody
    • Pre pro megakaryocyte potentiating factor antibody
    • Pre-pro-megakaryocyte-potentiating factor antibody
    • SMR antibody
    • SMRP antibody
    • Soluble MPF mesothelin related protein antibody
    see all

Anti-Mesothelin antibody [K1] images

  • All lanes : Anti-Mesothelin antibody [K1] (ab3362) at 1 µg/ml

    Lane 1 : Lung (Human) Tissue Lysate
    Lane 2 : Placenta (Human) Tissue Lysate - adult normal tissue (ab29745)

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilution
    developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 69 kDa
    Observed band size : 73 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 15 kDa,44 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 20 minutes

References for Anti-Mesothelin antibody [K1] (ab3362)

This product has been referenced in:
  • Wang K  et al. Inhibition of mesothelin as a novel strategy for targeting cancer cells. PLoS One 7:e33214 (2012). WB, Flow Cyt ; Human . Read more (PubMed: 22485139) »
  • Ren YR  et al. Structural Analysis of the Cancer-specific Promoter in Mesothelin and in Other Genes Overexpressed in Cancers. J Biol Chem 286:11960-9 (2011). WB ; Human . Read more (PubMed: 21288909) »

See all 7 Publications for this product

Product Wall

Attached is an image of mesothelin staining on FFPE ovarian tumor tissue. This product is sold as crude ascites fluid so we estimate the IgG concentration ˜1-3mg/ml. The precise concentration of 3.3 mg/ml on the datasheet is incorrect so I will change ...

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Thank you for your inquiry.



Great news! I heard back from the lab that we are actually in the process of making a new lot for inventory and have evaluated it for use on paraffin sections. We have used it successfully on formalin fi...

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This product recognizes the cellular form of Mesothelin. We have not tested to see if it can also detect the secreted form. We have only evaluated this for use in immunohistochemistry on frozen tissue sections. Flow cytometry and western blot data can ...

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Thank you for contacting Abcam.

I found some information regarding the conjugation for antibodies that contain BSA through the link below:
http://www.abcam.com/index.html?pageconfig=resource&rid=13156

Our tests have found that...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (pancreatic cancer)
Loading amount 40 µg
Specification pancreatic cancer
Gel Running Conditions Reduced Denaturing (10%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
Username

Dr. yunzhao ren

Verified customer

Submitted Jul 20 2011

Thank you for your enquiry. Antibodies ab15230 and ab3362 are different clones and were raised against different immunogens. ab15230 has been tested and shown to work using immunohistochemistry (Formalin-fixed paraffin-embedded sections), where...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"