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Spleen lymphocytes from mice fused with OVCAR-3, an ovarian tumorcell line derived from poorly differentiated adenocarcinoma of the ovary.
This product is sold as crude ascites fluid so we estimate the IgG concentration ~1-3mg/ml.
Our Abpromise guarantee covers the use of ab3362 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent concentration. Detects a band of approximately 73 kDa (predicted molecular weight: 69 kDa). PubMed: 18490724|
|IHC-Fr||Use at an assay dependent concentration.
Acetone fixation was used.
|Flow Cyt||Use at an assay dependent concentration. PubMed: 18490724|
|IHC-P||Use at an assay dependent concentration. Antigen retrieval is not essential but may optimise staining.
One hour primary antibody incubation was used.