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RabMAbs (2834-1)

Anti-Mesothelin antibody [PPI-2e(IHC)] (ab92347)

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Overview

  • Product nameAnti-Mesothelin antibody [PPI-2e(IHC)]See all Mesothelin primary antibodies ...
  • Description
    Rabbit monoclonal [PPI-2e(IHC)] to Mesothelin
  • Tested applicationsWB more details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    A synthetic peptide corresponding to residues near the C terminus of Human Mesothelin.

  • Positive controlFetal liver, uterus cancer, fetal pancreas and ovary cancer lysates.
  • General notesProduced under U.S. Patent No. 5,675,063.


    This antibody detects full-length Mesothelin precursor (69 kDa) and the C terminal cleaved form of Mesothelin.

Properties

Applications

Our Abpromise guarantee covers the use of ab92347 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
WB
  • Application notes
    WB: 1/1000 - 1/10000. Predicted molecular weight: 69 kDa.

    Is unsuitable for IHC-P or IP.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • Target

    • FunctionMembrane-anchored forms may play a role in cellular adhesion.
      Megakaryocyte-potentiating factor (MPF) potentiates megakaryocyte colony formation in vitro.
    • Tissue specificityExpressed in lung. Expressed at low levels in heart, placenta and kidney. Expressed in mesothelial cells. Highly expressed in mesotheliomas, ovarian cancers, and some squamous cell carcinomas (at protein level).
    • Involvement in diseaseNote=Antibodies against MSLN are detected in patients with mesothelioma and ovarian cancer.
    • Sequence similaritiesBelongs to the mesothelin family.
    • Post-translational
      modifications      Both MPF and the cleaved form of mesothelin are N-glycosylated.
      Proteolytically cleaved by a furin-like convertase to generate megakaryocyte-potentiating factor (MPF), and the cleaved form of mesothelin.
    • Cellular localizationSecreted and Cell membrane. Golgi apparatus.

      • Target information above from: UniProt accession Q13421 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links
      • Alternative names
          CAK 1 antibodyCAK1 antibodyCAK1 antigen antibody
          cleaved form antibodyMegakaryocyte potentiating factor antibodyMesothelin antibodyMesothelin isoform 1 precursor antibodyMPF antibodyMsln antibodyMSLN_HUMAN antibodyPre pro megakaryocyte potentiating factor antibodyPre-pro-megakaryocyte-potentiating factor antibodySMR antibodySMRP antibodySoluble MPF mesothelin related protein antibody
        see all

      Anti-Mesothelin antibody [PPI-2e(IHC)] images

      • Western blot - Mesothelin antibody [PPI-2e(IHC)] (ab92347)
        Western blot - Mesothelin antibody [PPI-2e(IHC)] (ab92347)
        All lanes : Anti-Mesothelin antibody [PPI-2e(IHC)] (ab92347) at 1/1000 dilution

        Lane 1 : Fetal liver lysate
        Lane 2 : Uterus cancer lysate
        Lane 3 : Fetal pancreas lysate
        Lane 4 : Ovary cancer lysate

        Lysates/proteins at 10 µg per lane.

        Secondary
        HRP labelled goat anti-rabbit antibody at 1/2000 dilution

        Predicted band size : 69 kDa

      References for Anti-Mesothelin antibody [PPI-2e(IHC)] (ab92347)

      ab92347 has not yet been referenced specifically in any publications.

      Publishing research using ab92347 ? Please let us know so that we can cite the reference in this datasheet

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      Thank you for your reply.



      Kindly advise the catalog No. of the below suggested products from Abcam, enabling us to inform the customer.



      Looking forward towards your valued response on it.


      Read More

      I have good news today.

      We now added the blocking peptide for ab92347 to our catalog and it is available for purchase.

      ab134675

      http://www.abcam.com/index.html?datasheet=134675 (or use the following: http://www.abcam.com/inde...

      Read More

      Thank you for your reply.



      Kindly advise the catalog No. of the below suggested products from Abcam, enabling us to inform the customer.



      Looking forward towards your valued response on it.


      Read More

      Thank you for your reply.

      I have to confirm that we do not have this peptide in our catalog at the moment. We are trying toadd this toour catalog but Ido not have any estimationwhen this will be available for your customer.

      Your custom...

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      Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"