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RabMAbs

Anti-Mesothelin antibody [SP74] (ab93620)

Submit an Abreview Q&A (5)

Overview

  • Product nameAnti-Mesothelin antibody [SP74]See all Mesothelin primary antibodies ...
  • Description
    Rabbit monoclonal [SP74] to Mesothelin
  • Tested applicationsIHC-P more details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    A synthetic peptide corresponding to C-terminus of human Mesothelin protein.

  • Positive controlMesothelioma.

Properties

Applications

Our Abpromise guarantee covers the use of ab93620 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
IHC-P
  • Application notesIHC-P: Use at an assay dependent dilution.

    Deparaffinization: Deparaffinize slides using xylene or xylene alternative and graded alcohols.

    Antibody Dilution: Dilute the antibody 1:100. The dilutions are estimates; actual results may differ because of variability in methods and protocols.

    Antigen Retrieval: Boil tissue section in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min.

    Primary Antibody Incubation: Incubate for 30 minutes at RT.

    Slide Washing: Slides must be washed in between steps. Rinse slides with PBS/0.05% Tween.

    Visualization: Detect the antibody as instructed by the instructions provided with the visualization system.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • Target

    • FunctionMembrane-anchored forms may play a role in cellular adhesion.
      Megakaryocyte-potentiating factor (MPF) potentiates megakaryocyte colony formation in vitro.
    • Tissue specificityExpressed in lung. Expressed at low levels in heart, placenta and kidney. Expressed in mesothelial cells. Highly expressed in mesotheliomas, ovarian cancers, and some squamous cell carcinomas (at protein level).
    • Involvement in diseaseNote=Antibodies against MSLN are detected in patients with mesothelioma and ovarian cancer.
    • Sequence similaritiesBelongs to the mesothelin family.
    • Post-translational
      modifications      Both MPF and the cleaved form of mesothelin are N-glycosylated.
      Proteolytically cleaved by a furin-like convertase to generate megakaryocyte-potentiating factor (MPF), and the cleaved form of mesothelin.
    • Cellular localizationSecreted and Cell membrane. Golgi apparatus.

      • Target information above from: UniProt accession Q13421 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links
      • Alternative names
          CAK 1 antibodyCAK1 antibodyCAK1 antigen antibody
          cleaved form antibodyMegakaryocyte potentiating factor antibodyMesothelin antibodyMesothelin isoform 1 precursor antibodyMPF antibodyMSLN antibodyMSLN_HUMAN antibodyPre pro megakaryocyte potentiating factor antibodyPre-pro-megakaryocyte-potentiating factor antibodySMR antibodySMRP antibodySoluble MPF mesothelin related protein antibody
        see all

      Anti-Mesothelin antibody [SP74] images

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Mesothelin antibody [SP74] (ab93620)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Mesothelin antibody [SP74] (ab93620)
        Ab93620 (1:100) staining Mesothelin in human esothelioma tissue by immunohistochemistry using formalin fixed, paraffin embedded tissue.

      References for Anti-Mesothelin antibody [SP74] (ab93620)

      ab93620 has not yet been referenced specifically in any publications.

      Publishing research using ab93620 ? Please let us know so that we can cite the reference in this datasheet

      Product Wall

      Submit an Abreview for ab93620 Submit a Question for ab93620

      Displaying 1 - 5 of 5 results for Abreviews and Q&A

      10
      Votes: Highest First

      I have gone through the site of http://mybiosource.com,(http://www.mybiosource.com/datasheet.php?products_id=427195&pfd=true) ,i have clear so many things by visiting that site, actuallythis mesothelin peptide has a corresponding antibody as i have re...

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      The peptide from mybiosource is a different peptide immunogen than the one used to produce our product ab93620. As I mentioned the exact immunogen sequence used to produce ab93620 is considered proprietary by our supplying lab however this peptide se...

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      in our experiment we are using an antibody (ab93620) dilution 1:100 times in PBS
      buffer as well as same or somewhat different antigen/protein
      concentration again in pbs buffer. then after making dilutions we
      check the specific binding of bo...

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      Unfortunately, we do not have any purified mesothelin for sale. I suggest consulting www.biocompare.com. It is a search service that has access to the catalogues of many companies, one of which may have the purified protein you need.

      I have required information about Mesothelin SP74 protein/ antigen,which should be corresponding / Non-corresponding to mesothelin (ab93620 ).

      The exact immunogen sequence is confidential. However I can say it is located within aa520-606 of Q13421 (Uniprot).

      I have required information for antigen against your abcam antibody Cat# ab93620..This antibody is raised in which source (Antigen OR Protein).

      Thanks for your reply.
      This antibody is raised against a small synthetic peptide of 15-20 amino acids.
      I hope this is the information you were looking for. Please contact me again if you have any further questions.

      Inquiry: I have required information about Mesothelin SP74 protein/ antigen,which should be corresponding / Non-corresponding to mesothelin (ab93620 ).

      Thank you for your enquiry but I don't quite understand what you are asking. Are you looking for a blocking peptide?
      Thanks in advance for clarification!

      Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"