RabMAb

Anti-Mesothelin antibody [SP74] (ab93620)

Overview

  • Product nameAnti-Mesothelin antibody [SP74]
    See all Mesothelin primary antibodies
  • Description
    Rabbit monoclonal [SP74] to Mesothelin
  • Tested applicationsIHC-P, Flow Cytmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Pig
  • Immunogen

    A synthetic peptide corresponding to C-terminus of human Mesothelin protein.

  • Positive control
    • Mesothelioma.

Properties

Applications

Our Abpromise guarantee covers the use of ab93620 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Flow Cyt Use at an assay dependent concentration.

Target

  • FunctionMembrane-anchored forms may play a role in cellular adhesion.
    Megakaryocyte-potentiating factor (MPF) potentiates megakaryocyte colony formation in vitro.
  • Tissue specificityExpressed in lung. Expressed at low levels in heart, placenta and kidney. Expressed in mesothelial cells. Highly expressed in mesotheliomas, ovarian cancers, and some squamous cell carcinomas (at protein level).
  • Involvement in diseaseNote=Antibodies against MSLN are detected in patients with mesothelioma and ovarian cancer.
  • Sequence similaritiesBelongs to the mesothelin family.
  • Post-translational
    modifications
    Both MPF and the cleaved form of mesothelin are N-glycosylated.
    Proteolytically cleaved by a furin-like convertase to generate megakaryocyte-potentiating factor (MPF), and the cleaved form of mesothelin.
  • Cellular localizationSecreted and Cell membrane. Golgi apparatus.
  • Information by UniProt
  • Database links
  • Alternative names
    • CAK 1 antibody
    • CAK1 antibody
    • CAK1 antigen antibody
    • cleaved form antibody
    • Megakaryocyte potentiating factor antibody
    • Mesothelin antibody
    • Mesothelin isoform 1 precursor antibody
    • MPF antibody
    • MSLN antibody
    • MSLN_HUMAN antibody
    • Pre pro megakaryocyte potentiating factor antibody
    • Pre-pro-megakaryocyte-potentiating factor antibody
    • SMR antibody
    • SMRP antibody
    • Soluble MPF mesothelin related protein antibody
    see all

Anti-Mesothelin antibody [SP74] images

  • Flow cytometric analysis of rabbit anti-Mesothelin (SP74) antibody ab93620(1/100) in HeLa cells (green) compared to negative control of rabbit IgG (blue).

  • Ab93620 (1:100) staining Mesothelin in human esothelioma tissue by immunohistochemistry using formalin fixed, paraffin embedded tissue.

References for Anti-Mesothelin antibody [SP74] (ab93620)

ab93620 has not yet been referenced specifically in any publications.

Product Wall


The peptide from mybiosource is a different peptide immunogen than the one used to produce our product ab93620. As I mentioned the exact immunogen sequence used to produce ab93620 is considered proprietary by our supplying lab however this peptide...

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Unfortunately, we do not have any purified mesothelin for sale. I suggest consulting www.biocompare.com. It is a search service that has access to the catalogues of many companies, one of which may have the purified protein you need.

The exact immunogen sequence is confidential. However I can say it is located within aa520-606 of Q13421 (Uniprot).

Thanks for your reply.
This antibody is raised against a small synthetic peptide of 15-20 amino acids.
I hope this is the information you were looking for. Please contact me again if you have any further questions.

Thank you for your enquiry but I don't quite understand what you are asking. Are you looking for a blocking peptide?
Thanks in advance for clarification!

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"