Overview
- Product nameAnti-Mesothelin antibodySee all Mesothelin primary antibodies ...
- DescriptionRabbit polyclonal to Mesothelin
- SpecificityDetects isoform 2.
- Tested applicationsWB more details
- Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Cow - Immunogen
Synthetic peptide corresponding to a region within the internal sequence amino acids 539-588 (QKLLGPHVEG LKAEERHRPV RDWILRQRQD DLDTLGLGLQ GGIPNGYLVL) of Human Mesothelin (NP_005814).
- Positive controlMCF7 cell lysate.
Properties
- FormLiquid
- Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
- Storage bufferPreservative: None
Constituents: 2% Sucrose, PBS -
Concentration information loading... - PurityImmunogen affinity purified
- Clonality Polyclonal
- IsotypeIgG
- Research Areas
Applications
Our Abpromise guarantee covers the use of ab83658 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| WB | WB: Use a concentration of 1 µg/ml. Detects a band of approximately 39 kDa (predicted molecular weight: 32 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T. |
Target
- FunctionMembrane-anchored forms may play a role in cellular adhesion.
Megakaryocyte-potentiating factor (MPF) potentiates megakaryocyte colony formation in vitro. - Tissue specificityExpressed in lung. Expressed at low levels in heart, placenta and kidney. Expressed in mesothelial cells. Highly expressed in mesotheliomas, ovarian cancers, and some squamous cell carcinomas (at protein level).
- Involvement in diseaseNote=Antibodies against MSLN are detected in patients with mesothelioma and ovarian cancer.
- Sequence similaritiesBelongs to the mesothelin family.
- Post-translational
modificationsBoth MPF and the cleaved form of mesothelin are N-glycosylated.
Proteolytically cleaved by a furin-like convertase to generate megakaryocyte-potentiating factor (MPF), and the cleaved form of mesothelin. - Cellular localizationSecreted and Cell membrane. Golgi apparatus.
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Database links
- Entrez Gene: 10232 Human
- Entrez Gene: 10232 Human
- Entrez Gene: 10232 Human
- Entrez Gene: 56047 Mouse
- Entrez Gene: 60333 Rat
- Omim: 601051 Human
- SwissProt: Q13421 Human
- SwissProt: Q13421 Human
- SwissProt: Q13421 Human
- SwissProt: Q61468 Mouse
- SwissProt: Q9ERA7 Rat
- Unigene: 408488 Human
- Unigene: 17510 Mouse
- Unigene: 18607 Rat
see all
Target information above from: UniProt accession
Q13421
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010)
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Alternative names
- CAK 1 antibodyCAK1 antibodyCAK1 antigen antibody
- cleaved form antibodyMegakaryocyte potentiating factor antibodyMesothelin antibodyMesothelin isoform 1 precursor antibodyMPF antibodyMsln antibodyMSLN_HUMAN antibodyPre pro megakaryocyte potentiating factor antibodyPre-pro-megakaryocyte-potentiating factor antibodySMR antibodySMRP antibodySoluble MPF mesothelin related protein antibody
see all
Anti-Mesothelin antibody images
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Western blot - Mesothelin antibody (ab83658)Anti-Mesothelin antibody (ab83658) at 1 µg/ml (in 5% skim milk/ PBS) + MCF7 cell lysate at 10 µg
Secondary
HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size : 32 kDa
Observed band size : 39 kDa (why is the actual band size different from the predicted?)
References for Anti-Mesothelin antibody (ab83658)
ab83658 has not yet been referenced specifically in any publications.
