Overview

  • Product name
  • Description
    Goat polyclonal to Mesothelin
  • Specificity
    This antibody is expected to recognize isoform 1 and 2 (NP_005814.2; NP_037536.2).
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide:

    CPSGKKAREIDES

    , corresponding to internal sequence amino acids 302-314 of Human Mesothelin (NP_005814.2; NP_037536.2).

  • Positive control
    • Human placenta lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab99474 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 70 kDa (predicted molecular weight: 69 kDa). An approximately 70 kDa band was observed in Human Placenta lysates. An additional band of unknown identity was also consistently observed at 150 kDa. This band was successfully blocked by incubation with the immunizing peptide.

Target

  • Function
    Membrane-anchored forms may play a role in cellular adhesion.
    Megakaryocyte-potentiating factor (MPF) potentiates megakaryocyte colony formation in vitro.
  • Tissue specificity
    Expressed in lung. Expressed at low levels in heart, placenta and kidney. Expressed in mesothelial cells. Highly expressed in mesotheliomas, ovarian cancers, and some squamous cell carcinomas (at protein level).
  • Involvement in disease
    Note=Antibodies against MSLN are detected in patients with mesothelioma and ovarian cancer.
  • Sequence similarities
    Belongs to the mesothelin family.
  • Post-translational
    modifications
    Both MPF and the cleaved form of mesothelin are N-glycosylated.
    Proteolytically cleaved by a furin-like convertase to generate megakaryocyte-potentiating factor (MPF), and the cleaved form of mesothelin.
  • Cellular localization
    Secreted and Cell membrane. Golgi apparatus.
  • Information by UniProt
  • Database links
  • Alternative names
    • CAK 1 antibody
    • CAK1 antibody
    • CAK1 antigen antibody
    • cleaved form antibody
    • Megakaryocyte potentiating factor antibody
    • Mesothelin antibody
    • Mesothelin isoform 1 precursor antibody
    • MPF antibody
    • Msln antibody
    • MSLN_HUMAN antibody
    • Pre pro megakaryocyte potentiating factor antibody
    • Pre-pro-megakaryocyte-potentiating factor antibody
    • SMR antibody
    • SMRP antibody
    • Soluble MPF mesothelin related protein antibody
    see all

Anti-Mesothelin antibody images

  • Anti-Mesothelin antibody (ab99474) at 0.3 µg/ml + Human placenta lysate in RIPA buffer at 35 µg
    Developed using the ECL technique

    Predicted band size : 69 kDa

References for Anti-Mesothelin antibody (ab99474)

ab99474 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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