Anti-Met (c-Met) antibody [3i20] (ab14571)

Overview

  • Product nameAnti-Met (c-Met) antibody [3i20]See all Met (c-Met) primary antibodies ...
  • Description
    Mouse monoclonal [3i20] to Met (c-Met)
  • SpecificityAb14571 detects endogenous levels of Met (c-Met) in various cell lines. It does not crossreact with any other related proteins.
  • Tested applicationsWB, IP more details
  • Species reactivity
    Reacts with: Mouse, Rat, Human, Monkey
  • Immunogen

    Synthetic peptide (KLH coupled) corresponding to residues surrounding Tyr1234 of human Met.

  • General notes


    Met (c-Met), a high affinity receptor for hepatocyte growth factor (HGF; also known as scatter factor), is a disulfide-linked heterodimer made of 45kD alpha and 145kD beta subunits. The alpha subunit and the amino-terminal region of beta-subunit form the extracellular domain. The remainder of the beta-chain spans the plasma membrane and contains a cytoplasmic region with tyrosine kinase activity. Interaction of Met (c-Met) with HGF results in autophosphorylation at multiple tyrosines, which recruit several downstream signaling components, including Gab1, c-Cbl and PI3 kinase and is critical for all of its known biological functions. Phosphorylation of Tyr1234/1235 in the Met kinase domain is critical to kinase activation. Phosphorylation of Tyr1349 in the Met (c-Met) cytoplasmic domain provides a direct binding site for Gab1. Altered Met (c-Met) levels and/or tyrosine kinase activities were found in different tumors, especially in renal, colon and breast cancers. Thus, Met (c-Met) is an attractive therapeutic target and cancer diagnostic.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, BSA, 150mM Sodium Chloride, 10mM HEPES. pH 7.5
  • Concentration information loading...
  • PurityAscites
  • Primary antibody notes Met (c-Met), a high affinity receptor for hepatocyte growth factor (HGF; also known as scatter factor), is a disulfide-linked heterodimer made of 45kD alpha and 145kD beta subunits. The alpha subunit and the amino-terminal region of beta-subunit form the extracellular domain. The remainder of the beta-chain spans the plasma membrane and contains a cytoplasmic region with tyrosine kinase activity. Interaction of Met (c-Met) with HGF results in autophosphorylation at multiple tyrosines, which recruit several downstream signaling components, including Gab1, c-Cbl and PI3 kinase and is critical for all of its known biological functions. Phosphorylation of Tyr1234/1235 in the Met kinase domain is critical to kinase activation. Phosphorylation of Tyr1349 in the Met (c-Met) cytoplasmic domain provides a direct binding site for Gab1. Altered Met (c-Met) levels and/or tyrosine kinase activities were found in different tumors, especially in renal, colon and breast cancers. Thus, Met (c-Met) is an attractive therapeutic target and cancer diagnostic.
  • Clonality Monoclonal
  • Clone number3i20
  • IsotypeIgG1
  • Research Areas

Applications

Our Abpromise guarantee covers the use of ab14571 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
WB 1/1000. Predicted molecular weight: 170 kDa.
IP 1/50.

Target

  • FunctionReceptor for hepatocyte growth factor and scatter factor. Has a tyrosine-protein kinase activity. Functions in cell proliferation, scattering, morphogenesis and survival.
  • Involvement in diseaseNote=Activation of MET after rearrangement with the TPR gene produces an oncogenic protein.
    Note=Defects in MET may be associated with gastric cancer.
    Defects in MET are a cause of hepatocellular carcinoma (HCC) [MIM:114550].
    Defects in MET are a cause of renal cell carcinoma papillary (RCCP) [MIM:605074]. It is a subtype of renal cell carcinoma tending to show a tubulo-papillary architecture formed by numerous, irregular, finger-like projections of connective tissue. Renal cell carcinoma is a heterogeneous group of sporadic or hereditary carcinoma derived from cells of the proximal renal tubular epithelium. It is subclassified into common renal cell carcinoma (clear cell, non-papillary carcinoma), papillary renal cell carcinoma, chromophobe renal cell carcinoma, collecting duct carcinoma with medullary carcinoma of the kidney, and unclassified renal cell carcinoma.
    Note=A common allele in the promoter region of the MET shows genetic association with susceptibility to autism in some families. Functional assays indicate a decrease in MET promoter activity and altered binding of specific transcription factor complexes.
    Note=MET activating mutations may be involved in the development of a highly malignant, metastatic syndrome known as cancer of unknown primary origin (CUP) or primary occult malignancy. Systemic neoplastic spread is generally a late event in cancer progression. However, in some instances, distant dissemination arises at a very early stage, so that metastases reach clinical relevance before primary lesions. Sometimes, the primary lesions cannot be identified in spite of the progresses in the diagnosis of malignancies.
  • Sequence similaritiesBelongs to the protein kinase superfamily. Tyr protein kinase family.
    Contains 3 IPT/TIG domains.
    Contains 1 protein kinase domain.
    Contains 1 Sema domain.
  • DomainThe kinase domain is involved in SPSB1 binding.
  • Post-translational
    modifications
    Dephosphorylated by PTPRJ at Tyr-1349 and Tyr-1365.
  • Cellular localizationMembrane.
  • Target information above from: UniProt accession P08581 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links
  • Alternative names
    • AUTS9 antibody
    • c met antibody
    • cmet antibody
    • D249 antibody
    • Hepatocyte growth factor receptor antibody
    • Hepatocyte growth factor receptor Precursor antibody
    • HGF antibody
    • HGF receptor antibody
    • HGF SF receptor antibody
    • HGF/SF receptor antibody
    • HGFR antibody
    • MET antibody
    • Met proto oncogene tyrosine kinase antibody
    • Met proto-oncogene (hepatocyte growth factor receptor) antibody
    • Met proto-oncogene antibody
    • Met protooncogene antibody
    • MET_HUMAN antibody
    • Oncogene MET antibody
    • Par4 antibody
    • Proto-oncogene c-Met antibody
    • RCCP2 antibody
    • Renal cell carcinoma papillary 2 gene antibody
    • Scatter factor receptor antibody
    • SF receptor antibody
    • Tyrosine-protein kinase Met antibody
    see all

Anti-Met (c-Met) antibody [3i20] images

  • All lanes : Anti-Met (c-Met) antibody [3i20] (ab14571) at 1/1000 dilution

    Lane 1 : Whole cell lysate prepared from mIMCD3 cells
    Lane 2 : Whole cell lysate prepared from African Green Monkey Vero cells.
    Lane 3 : Whole cell lysate prepared from HGF stimulated human 293 cells
    Lane 4 : Whole cell lysate prepared from serum starved human 293 cells
    Lane 5 : Whole cell lysate prepared from rat C6 cells.


    Predicted band size : 170 kDa

References for Anti-Met (c-Met) antibody [3i20] (ab14571)

ab14571 has not yet been referenced specifically in any publications.

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Thank you for your email.
We have the following antibodies for c-Met that have been tested in WB with rat samples (I assume that this is the species you wanted to work with, right?): ab47431.
We have several antibodies that are predicted to w...

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Thank you for submitting an Abreview of ab14571. As you may have noticed, your review has now been published on our website.
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Application Western blot
Sample Human Cell lysate - whole cell (HepG2 cell lysate)
Loading amount 20 µg
Specification HepG2 cell lysate
Gel Running Conditions Reduced Denaturing (10)
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 26°C
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Verified customer

Submitted Sep 14 2012

Thank you for sending the image of your results. The dots on the blot may be caused by either aggregation of your blocking agent or a dirty transfer sponge. Vortexing and filtering the blocking buffer, washing the transfer sponge, and using an addition...

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Thank you for your phone call. I am happy to hear that you are now getting the correct band size in your samples. If you could please send me an image of your blot, I will be happy to see if I can make any recommendations about the dots in the HepG2 po...

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I regret to inform you that there was a mistake on the datasheet for ab14571 and that zebrafish has not been tested with ab14571. If you can please provide your order details I will immediately arrange a refund and ask you to accept my sincere apolo...

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