Anti-Met (c-Met) antibody [ 4AT44] (ab59884)

Overview

• Product nameAnti-Met (c-Met) antibody [ 4AT44]See all Met (c-Met) primary antibodies ...
• Description
  Mouse monoclonal [ 4AT44] to Met (c-Met)
• Tested applicationsWB, ELISA, IHC-P more details
• Species reactivity
  Reacts with: Mouse, Human
  
• Immunogen

  Purified recombinant protein encoding the catalytic domain of human Met (c-Met).

• Positive controlHepG2 cell lysate.

Properties

• FormLiquid
• Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.
• Storage bufferPreservative: 0.09% Sodium Azide
  Constituents: PBS
• Concentration information loading...
• PurityProtein G purified
• Purification notesab59884 is purified through a protein G column and eluted out with both high and low pH buffers and neutralized immediately after elution then followed by dialysis against PBS.
• Clonality Monoclonal
• Clone number 4AT44
• IsotypeIgG1
• Research Areas
  • Cancer
  • Oncoproteins/suppressors
  • Oncoproteins
  • Growth factor receptors

  • Signal Transduction
  • Protein Phosphorylation
  • Tyrosine Kinases
  • Receptor Tyrosine Kinases

  • Epigenetics and Nuclear Signaling
  • Transcription
  • Cancer susceptibility
  • Proto-oncogenes

Applications

Target

• FunctionReceptor for hepatocyte growth factor and scatter factor. Has a tyrosine-protein kinase activity. Functions in cell proliferation, scattering, morphogenesis and survival.
• Involvement in diseaseNote=Activation of MET after rearrangement with the TPR gene produces an oncogenic protein.
  Note=Defects in MET may be associated with gastric cancer.
  Defects in MET are a cause of hepatocellular carcinoma (HCC) [MIM:114550].
  Defects in MET are a cause of renal cell carcinoma papillary (RCCP) [MIM:605074]. It is a subtype of renal cell carcinoma tending to show a tubulo-papillary architecture formed by numerous, irregular, finger-like projections of connective tissue. Renal cell carcinoma is a heterogeneous group of sporadic or hereditary carcinoma derived from cells of the proximal renal tubular epithelium. It is subclassified into common renal cell carcinoma (clear cell, non-papillary carcinoma), papillary renal cell carcinoma, chromophobe renal cell carcinoma, collecting duct carcinoma with medullary carcinoma of the kidney, and unclassified renal cell carcinoma.
  Note=A common allele in the promoter region of the MET shows genetic association with susceptibility to autism in some families. Functional assays indicate a decrease in MET promoter activity and altered binding of specific transcription factor complexes.
  Note=MET activating mutations may be involved in the development of a highly malignant, metastatic syndrome known as cancer of unknown primary origin (CUP) or primary occult malignancy. Systemic neoplastic spread is generally a late event in cancer progression. However, in some instances, distant dissemination arises at a very early stage, so that metastases reach clinical relevance before primary lesions. Sometimes, the primary lesions cannot be identified in spite of the progresses in the diagnosis of malignancies.
• Sequence similaritiesBelongs to the protein kinase superfamily. Tyr protein kinase family.
  Contains 3 IPT/TIG domains.
  Contains 1 protein kinase domain.
  Contains 1 Sema domain.
• DomainThe kinase domain is involved in SPSB1 binding.
• Post-translational
  modificationsDephosphorylated by PTPRJ at Tyr-1349 and Tyr-1365.
• Cellular localizationMembrane.

Target information above from: UniProt accession P08581 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 4233 Human
  • Entrez Gene: 17295 Mouse
  • Omim: 164860 Human
  • SwissProt: P08581 Human
  • SwissProt: P16056 Mouse
  • Unigene: 132966 Human
  • Unigene: 86844 Mouse

• Alternative names
  AUTS9 antibodyc met antibodycmet antibody

  D249 antibodyHepatocyte growth factor receptor antibodyHepatocyte growth factor receptor Precursor antibodyHGF antibodyHGF receptor antibodyHGF SF receptor antibodyHGF/SF receptor antibodyHGFR antibodyMET antibodyMet proto oncogene tyrosine kinase antibodyMet proto-oncogene (hepatocyte growth factor receptor) antibodyMet proto-oncogene antibodyMet protooncogene antibodyMET_HUMAN antibodyOncogene MET antibodyPar4 antibodyProto-oncogene c-Met antibodyRCCP2 antibodyRenal cell carcinoma papillary 2 gene antibodyScatter factor receptor antibodySF receptor antibodyTyrosine-protein kinase Met antibody

  see all

Anti-Met (c-Met) antibody [ 4AT44] images

• 

  Western blot - Met (c-Met) antibody (ab59884)
  Anti-Met (c-Met) antibody [ 4AT44] (ab59884) at 1/100 dilution + HepG2 cell lysate at 10 µg
  
  Predicted band size : 156 kDa
  Observed band size : 135 kDa (why is the actual band size different from the predicted?)
  
• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Met (c-Met) antibody [ 4AT44] (ab59884)

  ab59884 at 1/100 dilution staining Met (c-Met) in human colon carcinoma tissue section by Immunohistochemistry (Formalin/ PFA fixed paraffin-embedded sections). A peroxidase conjugated secondary antibody was used followed by DAB staining.