RabMAb

Anti-Met (c-Met) antibody [EP1454Y] (ab51067)

Overview

  • Product nameAnti-Met (c-Met) antibody [EP1454Y]See all Met (c-Met) primary antibodies ...
  • Description
    Rabbit monoclonal [EP1454Y] to Met (c-Met)
  • Tested applicationsWB, IHC-P, ICC/IF, Flow Cytmore details
  • Species reactivity
    Reacts with: Human

    Does not react with

    Mouse, Rat
  • Immunogen

    Synthetic peptide corresponding to residues near the N-terminus of human Met (c-Met).

  • Positive control
    • 293 cell lysate or human breast carcinoma tissue.
  • General notes

    Produced under U.S. Patent No. 5,675,063. A 40 μl trial size is available to purchase for this antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab51067 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 160 kDa (predicted molecular weight: 156 kDa).Can be blocked with Met (c-Met) peptide (ab167073).
IHC-P 1/100 - 1/250.
ICC/IF 1/100 - 1/250.
Flow Cyt 1/1000.
  • Application notesIs unsuitable for IP.
  • Target

    • FunctionReceptor for hepatocyte growth factor and scatter factor. Has a tyrosine-protein kinase activity. Functions in cell proliferation, scattering, morphogenesis and survival.
    • Involvement in diseaseNote=Activation of MET after rearrangement with the TPR gene produces an oncogenic protein.
      Note=Defects in MET may be associated with gastric cancer.
      Defects in MET are a cause of hepatocellular carcinoma (HCC) [MIM:114550].
      Defects in MET are a cause of renal cell carcinoma papillary (RCCP) [MIM:605074]. It is a subtype of renal cell carcinoma tending to show a tubulo-papillary architecture formed by numerous, irregular, finger-like projections of connective tissue. Renal cell carcinoma is a heterogeneous group of sporadic or hereditary carcinoma derived from cells of the proximal renal tubular epithelium. It is subclassified into common renal cell carcinoma (clear cell, non-papillary carcinoma), papillary renal cell carcinoma, chromophobe renal cell carcinoma, collecting duct carcinoma with medullary carcinoma of the kidney, and unclassified renal cell carcinoma.
      Note=A common allele in the promoter region of the MET shows genetic association with susceptibility to autism in some families. Functional assays indicate a decrease in MET promoter activity and altered binding of specific transcription factor complexes.
      Note=MET activating mutations may be involved in the development of a highly malignant, metastatic syndrome known as cancer of unknown primary origin (CUP) or primary occult malignancy. Systemic neoplastic spread is generally a late event in cancer progression. However, in some instances, distant dissemination arises at a very early stage, so that metastases reach clinical relevance before primary lesions. Sometimes, the primary lesions cannot be identified in spite of the progresses in the diagnosis of malignancies.
    • Sequence similaritiesBelongs to the protein kinase superfamily. Tyr protein kinase family.
      Contains 3 IPT/TIG domains.
      Contains 1 protein kinase domain.
      Contains 1 Sema domain.
    • DomainThe kinase domain is involved in SPSB1 binding.
    • Post-translational
      modifications
      Dephosphorylated by PTPRJ at Tyr-1349 and Tyr-1365.
    • Cellular localizationMembrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • AUTS9 antibody
      • c met antibody
      • cmet antibody
      • D249 antibody
      • Hepatocyte growth factor receptor antibody
      • Hepatocyte growth factor receptor Precursor antibody
      • HGF antibody
      • HGF receptor antibody
      • HGF SF receptor antibody
      • HGF/SF receptor antibody
      • HGFR antibody
      • MET antibody
      • Met proto oncogene tyrosine kinase antibody
      • Met proto-oncogene (hepatocyte growth factor receptor) antibody
      • Met proto-oncogene antibody
      • Met protooncogene antibody
      • MET_HUMAN antibody
      • Oncogene MET antibody
      • Par4 antibody
      • Proto-oncogene c-Met antibody
      • RCCP2 antibody
      • Renal cell carcinoma papillary 2 gene antibody
      • Scatter factor receptor antibody
      • SF receptor antibody
      • Tyrosine-protein kinase Met antibody
      see all

    Anti-Met (c-Met) antibody [EP1454Y] images

    • Overlay histogram showing Jurkat cells stained with ab51067 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab51067, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
    • Anti-Met (c-Met) antibody [EP1454Y] (ab51067) at 1/2000 dilution + 293 cell lysate at 10 µg

      Secondary
      Goat anti-Rabbit HRP labeled at 1/2000 dilution

      Predicted band size : 156 kDa
      Observed band size : 160 kDa (why is the actual band size different from the predicted?)
    • Ab51067 (1/100-1/250) staining human Met (c-Met) in human breast carcinoma tissue by immunohistochemistry using paraffin embedded tissue.Ab51067 (1/100-1/250) staining human Met (c-Met) in human breast carcinoma tissue by immunohistochemistry using paraffin embedded tissue.
    • ICC/IF image of ab51067 stained HepG2 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab51067, 1/1000 dilution) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

    • ab51067 showing positive staining in Hepatocellular carcinoma tissue.

    • ab51067 showing positive staining in Thyroid gland carcinoma tissue.

    • ab51067 showing positive staining in Normal tonsil tissue.

    References for Anti-Met (c-Met) antibody [EP1454Y] (ab51067)

    This product has been referenced in:
    • Terwisscha van Scheltinga AG  et al. In vivo visualization of MET tumor expression and anticalin biodistribution with the MET-specific anticalin 89Zr-PRS-110 PET tracer. J Nucl Med 55:665-71 (2014). IHC ; Human . Read more (PubMed: 24614223) »
    • Jacobsen F  et al. High c-MET expression is frequent but not associated with early PSA recurrence in prostate cancer. Exp Ther Med 5:102-106 (2013). IHC-P ; Human . Read more (PubMed: 23251249) »

    See all 9 Publications for this product

    Product Wall

    Thank you for contacting us.
    The isotype controls are as follows:
    ab8158 -- ab18450 rat IgG2a
    ab51067 -- ab125938 rabbit monoclonal
    ab28364 -- ab27472 rabbit polyclonal
    I hope this information is helpful to you. Please do not h...

    Read More
    Application Flow Cytometry
    Fixation Paraformaldehyde
    Permeabilization Yes - 0.1% Triton in PBS
    Sample Human Cell (MCF-7 (invasive ductal carcinoma line))
    Specification MCF-7 (invasive ductal carcinoma line)
    Gating Strategy isotype control (black line in histogram)
    Preparation Cell harvesting/tissue preparation method: accutase and accumax dissociation
    Sample buffer: PBS 7.4 + 10% FBS for washing
    Username

    Abcam user community

    Verified customer

    Submitted Mar 07 2014


    The anti-Met antibody ab51067 was tested on mouse tissue lysates (brain, heart, kidney and spleen) in western blotting, but the antibody has not been tested on mouse tissues by IHC.

    Although it was negative with the above mouse tissue, ...

    Read More
    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citrate buffer, pH6
    Sample Human Tissue sections (human gastric cancer xenograft)
    Specification human gastric cancer xenograft
    Blocking step BSA as blocking agent for 20 minute(s) · Concentration: 1% · Temperature: 25°C
    Fixative Formaldehyde
    Username

    Abcam user community

    Verified customer

    Submitted May 23 2013


    The blocking peptide for ab51067 has been added to our catalog as ab167073.
    http://www.abcam.com/index.html?datasheet=167073

    I can confirm that ab51067 is sold as tissue culture supernatant. Unpurified antibodies, such as those sold as whole antiserum, ascites or tissue culture supernatant will not have a concentration stated on the datasheet. Antibody concentration is usual...

    Read More

    Thank you for contacting us.

    I have been unable to find further information regarding the precursor C-Met which you have mentioned. However I can provide the immunogen sequence for this product which will hopefully help you to determine th...

    Read More

    As shown on the product datasheet http://www.abcam.com/ab51067 thisantibody is sold as tissue culture supernatant. Unpurified antibodies, such as those sold as whole antiserum, ascites or tissue culture supernatant will not have a concentration stated ...

    Read More

    Thank you for contacting Abcam.
    The concentration for ab51067, lot #GR57435-3 is *********mg/mL.
    Please let me know if there is anything else we can help you with.

    Thank you for contacting us. The immunogen shares 94% identity with mouse cMet. However, we did not detect any bands in mouse tissue lysates (brain, heart, kidney, and spleen). Thus, I would not expect cross-reactivity with mouse. I hope this is helpfu...

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"