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APYPSLLPSQDNIDGEGNTconjugated to Ovalbumin, corresponding to amino acids 1361-1379 of Mouse Met (c-Met). The immunizing sequence has 18 of 19 identical amino acids in rat.
Met (c-Met), a high affinity receptor for hepatocyte growth factor (HGF; also known as scatter factor), is a disulfide-linked heterodimer made of 45kD alpha and 145kD beta subunits. The alpha subunit and the amino-terminal region of beta-subunit form the extracellular domain. The remainder of the beta-chain spans the plasma membrane and contains a cytoplasmic region with tyrosine kinase activity. Interaction of Met (c-Met) with HGF results in autophosphorylation at multiple tyrosines, which recruit several downstream signaling components, including Gab1, c-Cbl and PI3 kinase and is critical for all of its known biological functions. Phosphorylation of Tyr1234/1235 in the Met kinase domain is critical to kinase activation. Phosphorylation of Tyr1349 in the Met (c-Met) cytoplasmic domain provides a direct binding site for Gab1. Altered Met (c-Met) levels and/or tyrosine kinase activities were found in different tumors, especially in renal, colon and breast cancers. Thus, Met (c-Met) is an attractive therapeutic target and cancer diagnostic.
Our Abpromise guarantee covers the use of ab14570 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use at an assay dependent concentration.|
|WB||Use a concentration of 2 µg/ml. Predicted molecular weight: 170 kDa.
ab14570 detects Met (c-Met) in mouse liver membrane fractions using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system. The antibody recognises a doublet at 170 and 140kDa as well as a non-specific protein at ~45kDa
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