Anti-Met (c-Met) antibody (ab39075)

Overview

• Product nameAnti-Met (c-Met) antibodySee all Met (c-Met) primary antibodies ...
• Description
  Rabbit polyclonal to Met (c-Met)
• Tested applicationsIHC-P, WB more details
• Species reactivity
  Reacts with: Human
  Predicted to work with: Mouse, Rat, Sheep, Rabbit, Horse, Guinea pig, Cow, Cat, Dog, Pig, a wide range of other species
• Immunogen

  Synthetic peptide conjugated to KLH derived from within residues 1000 - 1100 Met (c-Met).

  Read Abcam's proprietary immunogen policy

  (Peptide available as ab39074.)

• Positive controlHeLa (Human epithelial carcinoma cell line) Whole Cell Lysate This antibody gave a positive result in IHC in the following FFPE tissue: Human lung adenocarcinoma.

Properties

• FormLiquid
• Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
• Storage bufferPreservative: 0.02% Sodium Azide
  Constituents: 1% BSA, PBS, pH 7.4
• Concentration information loading...
• PurityImmunogen affinity purified
• Clonality Polyclonal
• IsotypeIgG
• Research Areas
  • Cancer
  • Oncoproteins/suppressors
  • Oncoproteins
  • Growth factor receptors

  • Signal Transduction
  • Protein Phosphorylation
  • Tyrosine Kinases
  • Receptor Tyrosine Kinases

  • Epigenetics and Nuclear Signaling
  • Transcription
  • Cancer susceptibility
  • Proto-oncogenes

Applications

Target

• FunctionReceptor for hepatocyte growth factor and scatter factor. Has a tyrosine-protein kinase activity. Functions in cell proliferation, scattering, morphogenesis and survival.
• Involvement in diseaseNote=Activation of MET after rearrangement with the TPR gene produces an oncogenic protein.
  Note=Defects in MET may be associated with gastric cancer.
  Defects in MET are a cause of hepatocellular carcinoma (HCC) [MIM:114550].
  Defects in MET are a cause of renal cell carcinoma papillary (RCCP) [MIM:605074]. It is a subtype of renal cell carcinoma tending to show a tubulo-papillary architecture formed by numerous, irregular, finger-like projections of connective tissue. Renal cell carcinoma is a heterogeneous group of sporadic or hereditary carcinoma derived from cells of the proximal renal tubular epithelium. It is subclassified into common renal cell carcinoma (clear cell, non-papillary carcinoma), papillary renal cell carcinoma, chromophobe renal cell carcinoma, collecting duct carcinoma with medullary carcinoma of the kidney, and unclassified renal cell carcinoma.
  Note=A common allele in the promoter region of the MET shows genetic association with susceptibility to autism in some families. Functional assays indicate a decrease in MET promoter activity and altered binding of specific transcription factor complexes.
  Note=MET activating mutations may be involved in the development of a highly malignant, metastatic syndrome known as cancer of unknown primary origin (CUP) or primary occult malignancy. Systemic neoplastic spread is generally a late event in cancer progression. However, in some instances, distant dissemination arises at a very early stage, so that metastases reach clinical relevance before primary lesions. Sometimes, the primary lesions cannot be identified in spite of the progresses in the diagnosis of malignancies.
• Sequence similaritiesBelongs to the protein kinase superfamily. Tyr protein kinase family.
  Contains 3 IPT/TIG domains.
  Contains 1 protein kinase domain.
  Contains 1 Sema domain.
• DomainThe kinase domain is involved in SPSB1 binding.
• Post-translational
  modificationsDephosphorylated by PTPRJ at Tyr-1349 and Tyr-1365.
• Cellular localizationMembrane.

Target information above from: UniProt accession P08581 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 403438 Dog
  • Entrez Gene: 4233 Human
  • Entrez Gene: 17295 Mouse
  • Entrez Gene: 24553 Rat
  • Omim: 164860 Human
  • SwissProt: Q75ZY9 Dog
  • SwissProt: P08581 Human
  • SwissProt: P16056 Mouse

  • SwissProt: P97523 Rat
  • Unigene: 132966 Human
  • Unigene: 86844 Mouse
  • Unigene: 10617 Rat

  see all

• Alternative names
  AUTS9 antibodyc met antibodycmet antibody

  cmet antibodyD249 antibodyHepatocyte growth factor receptor antibodyHepatocyte growth factor receptor Precursor antibodyHepatocyte growth factor receptor Precursor antibodyHGF antibodyHGF receptor antibodyHGF SF receptor antibodyHGF SF receptor antibodyHGF/SF receptor antibodyHGFR antibodyMET antibodyMet proto oncogene tyrosine kinase antibodyMet proto-oncogene (hepatocyte growth factor receptor) antibodyMet proto-oncogene antibodyMet protooncogene antibodyMET_HUMAN antibodyOncogene MET antibodyPar4 antibodyProto-oncogene c-Met antibodyRCCP2 antibodyRenal cell carcinoma papillary 2 gene antibodyRenal cell carcinoma papillary 2 gene antibodyScatter factor receptor antibodySF receptor antibodyTyrosine-protein kinase Met antibody

  see all

Anti-Met (c-Met) antibody images

• 

  Western blot - Met (c-Met) antibody (ab39075)
  All lanes : Anti-Met (c-Met) antibody (ab39075) at 1/250 dilution
  
  Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
  Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate (ab7899)
  Lane 3 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate (ab7909)
  
  Lysates/proteins at 10 µg per lane.
  
  Secondary
  IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
  
  Performed under reducing conditions.
  
  Predicted band size : 155 kDa
  Observed band size : 145 kDa (why is the actual band size different from the predicted?)
  
• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Met (c-Met) antibody (ab39075)

  IHC image of Met (c-Met) staining in Human lung adenocarcinoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab39075, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

  For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.