Overview

  • Product nameMet (pY1234/pY1235) ELISA Kit
  • Detection methodColorimetric
  • Tests
    1 x 96 well plate
  • Sample type
    Cell Lysate
  • Assay typeSandwich (qualitative)
  • Assay time
    5h 00m
  • Assay durationMultiple steps standard assay
  • Species reactivity
    Reacts with: Mouse, Human
  • Product overview

    ab126450 is a very rapid, convenient and sensitive assay kit that can monitor the activation or function of important biological pathways in Human and Mouse cell lysates. By determining phosphorylated Met protein in your experimental model system, you can verify pathway activation in your cell lysates. You can simultaneously measure numerous different cell lysates without spending excess time and effort in performing a Western Blotting analysis.

    This Sandwich ELISA kit is an in vitro enzyme-linked immunosorbent assay for the measurement of Human and Mouse phospho-Met (Tyr1234/1235). An anti-pan Met antibody has been coated onto a 96-well plate. Samples are pipetted into the wells and Met present in a sample is bound to the wells by the immobilized antibody. The wells are washed and anti-Met (Tyr1234/1235) antibody is used to detect phosphorylated Met (Tyr1234/1235). After washing away unbound antibody, HRP-conjugated anti-Rabbit IgG is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Met (Tyr1234/1235) bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • Tested applicationsSandwich ELISA more details
  • PlatformMicroplate

Properties

  • FunctionReceptor for hepatocyte growth factor and scatter factor. Has a tyrosine-protein kinase activity. Functions in cell proliferation, scattering, morphogenesis and survival.
  • Involvement in diseaseNote=Activation of MET after rearrangement with the TPR gene produces an oncogenic protein.
    Note=Defects in MET may be associated with gastric cancer.
    Defects in MET are a cause of hepatocellular carcinoma (HCC) [MIM:114550].
    Defects in MET are a cause of renal cell carcinoma papillary (RCCP) [MIM:605074]. It is a subtype of renal cell carcinoma tending to show a tubulo-papillary architecture formed by numerous, irregular, finger-like projections of connective tissue. Renal cell carcinoma is a heterogeneous group of sporadic or hereditary carcinoma derived from cells of the proximal renal tubular epithelium. It is subclassified into common renal cell carcinoma (clear cell, non-papillary carcinoma), papillary renal cell carcinoma, chromophobe renal cell carcinoma, collecting duct carcinoma with medullary carcinoma of the kidney, and unclassified renal cell carcinoma.
    Note=A common allele in the promoter region of the MET shows genetic association with susceptibility to autism in some families. Functional assays indicate a decrease in MET promoter activity and altered binding of specific transcription factor complexes.
    Note=MET activating mutations may be involved in the development of a highly malignant, metastatic syndrome known as cancer of unknown primary origin (CUP) or primary occult malignancy. Systemic neoplastic spread is generally a late event in cancer progression. However, in some instances, distant dissemination arises at a very early stage, so that metastases reach clinical relevance before primary lesions. Sometimes, the primary lesions cannot be identified in spite of the progresses in the diagnosis of malignancies.
  • Sequence similaritiesBelongs to the protein kinase superfamily. Tyr protein kinase family.
    Contains 3 IPT/TIG domains.
    Contains 1 protein kinase domain.
    Contains 1 Sema domain.
  • DomainThe kinase domain is involved in SPSB1 binding.
  • Post-translational
    modifications
    Dephosphorylated by PTPRJ at Tyr-1349 and Tyr-1365.
  • Cellular localizationMembrane.
  • Target information above from: UniProt accession P08581 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Alternative names
    • AUTS9
    • c Met
    • Hepatocyte growth factor receptor
    • HGF receptor
    • HGF/SF receptor
    • HGFR
    • MET
    • Met proto oncogene
    • Met proto oncogene tyrosine kinase
    • MET_HUMAN
    • Oncogene MET
    • Proto-oncogene c-Met
    • RCCP2
    • Scatter factor receptor
    • SF receptor
    • Tyrosine-protein kinase Met
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab126450 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
Sandwich ELISA Use at an assay dependent concentration.

Met (pY1234/pY1235) ELISA Kit images

  • H1993 cells were cultured at 37°C for 4 days. Solubilize cells at 4 x 107 cells/ml in Cell Lysate Buffer. Serial dilutions of lysates were analyzed using ab126450.
  • A431 cells were treated or untreated with 50 ng/ml recombinant Human HGF for 5 min. Cell lysates were analyzed using ab126450.
  • H1993 cells were cultured at 37°C for 4 days. Solubilize cells at 4 x 107 cells/ml in Cell Lysate Buffer. Serial dilutions of lysates were analyzed using ab126450.
  • H1993 cells were cultured at 37°C for 4 days. Solubilize cells at 4 x 107 cells/ml in Cell Lysate Buffer. Serial dilutions of lysates were analyzed by Western blot.

Protocols

References for Met (pY1234/pY1235) ELISA Kit (ab126450)

ab126450 has not yet been referenced specifically in any publications.

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