Met (pY1234/pY1235) ELISA Kit (ab126450)
Overview
- Product nameMet (pY1234/pY1235) ELISA Kit
- Tests1 x 96 well plate
- Sample typeCell Lysate
- Assay typeSandwich
- Assay time5h 00m
- Species reactivityReacts with: Mouse, Human
- Product overview
ab126450 is a very rapid, convenient and sensitive assay kit that can monitor the activation or function of important biological pathways in Human and Mouse cell lysates. By determining phosphorylated Met protein in your experimental model system, you can verify pathway activation in your cell lysates. You can simultaneously measure numerous different cell lysates without spending excess time and effort in performing a Western Blotting analysis.
This Sandwich ELISA kit is an in vitro enzyme-linked immunosorbent assay for the measurement of Human and Mouse phospho-Met (Tyr1234/1235). An anti-pan Met antibody has been coated onto a 96-well plate. Samples are pipetted into the wells and Met present in a sample is bound to the wells by the immobilized antibody. The wells are washed and anti-Met (Tyr1234/1235) antibody is used to detect phosphorylated Met (Tyr1234/1235). After washing away unbound antibody, HRP-conjugated anti-Rabbit IgG is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Met (Tyr1234/1235) bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
- Tested applicationsSandwich ELISA more details
Properties
- Storage instructionsStore at -20°C. Please refer to protocols.
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Components Identifier 1 x 96 tests 20X Wash Buffer Concentrate Item B 1 x 25ml 2X Cell Lysate Buffer Item J 1 x 5ml Assay Diluent: 5x concentrated buffer 1 x 15ml Detection Antibody Met (Tyr1234/1235) 2 vials HRP-conjugated anti-rabbit IgG: 1000x HRP-conjugated anti-rabbit IgG concentrate 1 x 25µl Met Microplate (12 strips x 8 wells) coated with anti-pan Met antibody 1 unit Positive Control: lyophilized powder from H1993 cell lysate 2 vials Stop Solution Item I 1 x 8ml TMB One-Step Substrate Reagent Item H 1 x 12ml -
Research Areas
Note=Defects in MET may be associated with gastric cancer.
Defects in MET are a cause of hepatocellular carcinoma (HCC) [MIM:114550].
Defects in MET are a cause of renal cell carcinoma papillary (RCCP) [MIM:605074]. It is a subtype of renal cell carcinoma tending to show a tubulo-papillary architecture formed by numerous, irregular, finger-like projections of connective tissue. Renal cell carcinoma is a heterogeneous group of sporadic or hereditary carcinoma derived from cells of the proximal renal tubular epithelium. It is subclassified into common renal cell carcinoma (clear cell, non-papillary carcinoma), papillary renal cell carcinoma, chromophobe renal cell carcinoma, collecting duct carcinoma with medullary carcinoma of the kidney, and unclassified renal cell carcinoma.
Note=A common allele in the promoter region of the MET shows genetic association with susceptibility to autism in some families. Functional assays indicate a decrease in MET promoter activity and altered binding of specific transcription factor complexes.
Note=MET activating mutations may be involved in the development of a highly malignant, metastatic syndrome known as cancer of unknown primary origin (CUP) or primary occult malignancy. Systemic neoplastic spread is generally a late event in cancer progression. However, in some instances, distant dissemination arises at a very early stage, so that metastases reach clinical relevance before primary lesions. Sometimes, the primary lesions cannot be identified in spite of the progresses in the diagnosis of malignancies.
Contains 3 IPT/TIG domains.
Contains 1 protein kinase domain.
Contains 1 Sema domain.
modificationsDephosphorylated by PTPRJ at Tyr-1349 and Tyr-1365.
Target information above from: UniProt accession
P08581
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010)
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Alternative names
- AUTS9c MetHepatocyte growth factor receptor
- HGF receptorHGF/SF receptorHGFRMETMet proto oncogeneMet proto oncogene tyrosine kinaseMET_HUMANOncogene METProto-oncogene c-MetRCCP2Scatter factor receptorSF receptorTyrosine-protein kinase Met
see all
- Entrez Gene: 4233 Human
- Entrez Gene: 17295 Mouse
- Omim: 164860 Human
- SwissProt: P08581 Human
- SwissProt: P16056 Mouse
- Unigene: 132966 Human
- Unigene: 86844 Mouse
Applications
Our Abpromise guarantee covers the use of ab126450 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| Sandwich ELISA | sELISA |
Met (pY1234/pY1235) ELISA Kit images
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Sandwich ELISA - Met (pY1234/pY1235) ELISA Kit (ab126450)H1993 cells were cultured at 37°C for 4 days. Solubilize cells at 4 x 107 cells/ml in Cell Lysate Buffer. Serial dilutions of lysates were analyzed using ab126450. -
Sandwich ELISA - Met (pY1234/pY1235) ELISA Kit (ab126450)A431 cells were treated or untreated with 50 ng/ml recombinant Human HGF for 5 min. Cell lysates were analyzed using ab126450. -
Sandwich ELISA - Met (pY1234/pY1235) ELISA Kit (ab126450)H1993 cells were cultured at 37°C for 4 days. Solubilize cells at 4 x 107 cells/ml in Cell Lysate Buffer. Serial dilutions of lysates were analyzed using ab126450. -
Western blot - Met (pY1234/pY1235) ELISA Kit (ab126450)H1993 cells were cultured at 37°C for 4 days. Solubilize cells at 4 x 107 cells/ml in Cell Lysate Buffer. Serial dilutions of lysates were analyzed by Western blot.
Protocols
References for Met (pY1234/pY1235) ELISA Kit (ab126450)
ab126450 has not yet been referenced specifically in any publications.
