MetaPath™ Fatty Acid Oxidation 4-Plex Dipstick Array (ab109880)

Overview

• Product nameMetaPath™ Fatty Acid Oxidation 4-Plex Dipstick Array
• Sample type
  Cell culture extracts, Tissue
• Assay typeQuantitative
• Assay time
  0h 30m
• Species reactivity
  Reacts with: Human
  
• Product overview

  ab109880 (MSX32) is a novel array that allows for the simultaneous quantification of 3 key enzymes involved in fatty acid oxidation: MCAD, SCHAD and Mitochondrial Trifunctional Protein. Frataxin is also included as a control.

  The array is comprised of 4 monoclonal capture antibodies striped onto a dipstick, and the immunocaptured sample is detected using 4 monoclonal detector antibodies that recognize different epitopes on the target enzymes.

  This array is very rapid (total assay time including sample prep is less than 1 hour) and very sensitive, and as with all Abcam's assays, isolation of mitochondria is not required.

  This array is suitable for testing a variety of sample types. Not only is it useful for measuring samples that are deficient in the 3 enzymes due to genetic mutations, it is also useful for measuring changes in the expression of the enzymes due to drug treatments or other manipulations.

• Notes

  All components are stable in their provided containers at room temperature out of direct sunlight.

  After diluting the 10X Blocking Buffer to 2X, store at 4°C.

  For long-term storage, all buffers can be stored at 4°C.

• Tested applicationsSandwich ELISA more details

Properties

• Storage instructionsStore at +4°C. Please refer to protocols.

• Components	90 tests	30 tests
  Buffer B (10X Blocking solution)	3 x 0.4ml	1 x 0.4ml
  Buffer C (Wash buffer)	6 x 1ml	2 x 1ml
  Dipsticks	3 x 30 units	1 x 30 units
  Extraction Buffer	3 x 15ml	1 x 15ml
  Gold-conjugated antibody (dried in microplate wells)	3 x 30 tests	1 x 30 tests

• Research Areas
  • Metabolism
  • Pathways and Processes
  • Metabolic signaling pathways
  • Lipid and lipoprotein metabolism
  • Lipid metabolism

  • Metabolism
  • Pathways and Processes
  • Redox metabolism
  • Fatty acid oxidation

  • Metabolism
  • Pathways and Processes
  • Metabolic signaling pathways
  • Lipid and lipoprotein metabolism
  • Fatty acids

  • Cancer
  • Cancer Metabolism
  • Metabolic signaling pathway
  • Metabolism of lipids and lipoproteins

  • Kits/ Lysates/ Other
  • Kits
  • Cell Metabolism Kits
  • Fatty Acid Assays

  • Kits/ Lysates/ Other
  • Kits
  • Cell Metabolism Kits
  • Lipid Metabolism Kits
  • Fatty acids

  • Signal Transduction
  • Metabolism
  • Lipid metabolism

  • Cardiovascular
  • Lipids / Lipoproteins
  • Fatty Acids
  • Metabolism

Applications

MetaPath™ Fatty Acid Oxidation 4-Plex Dipstick Array images

• 

  - MetaPath™ Fatty Acid Oxidation 4-Plex Dipstick Array (ab109880)
  Measurement of patient and deficient cell lines. Untransformed fibroblasts cell lines cultured from patients with lipid metabolism disorders were analyzed by these dipsticks. Absorbance units were compared to neonatal skin fibroblast cells (1) as a control. Patient cell line (2) has TFP protein deficiency likely a result of mutations R61H (182G-A) and R247H (740G-A) in HADHB gene (TFP beta subunit). While cell lines (3) and (4) both have MCAD activity deficiency as a result of the prevalent MCAD mutation K304E, 985A-G) in the ACADM gene. Cell lines patients deficient in lipid metabolism were analyzed. Note the mAbs units measured for the untransformed control fibroblasts are different from the transformed HepG2 liver cell lines shown above. Also note a consistent decreased SCHAD level in these patients cell lines which may be a consequence of general lipid metabolism disruption in these patient cell lines.
• 

  Sandwich ELISA - MetaPath™ Fatty Acid Oxidation 4-Plex Dipstick Array (ab109880)
  Abcam's enzyme activity assays apply a novel approach, whereby target enzymes are first immunocaptured from tissue or cell samples before subsequent functional analysis. Dipstick ELISA Kits extend this concept by utilizing the well-established lateral flow concept, wherein capture antibodies are striped onto nitrocellulose membrane and a wicking pad draws the sample through the antibody bands. All of our ELISA kits utilize highly validated monoclonal antibodies (learn more about validation) and proprietary buffers, which are able to capture even very large enzyme complexes in their fully-intact, functionally-active states.

Protocols

Protocol Booklet