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Read our guarantee »Overview
Product name
MetaPath™ Mito Disease 4-Plex Dipstick Array
Sample type
Cell culture extracts, Tissue
Assay type
Quantitative
Assay time
0h 30m
Cross reactivity
Reacts with
Human
Product overview
ab109879 (MSX31) is a novel array that allows for the simultaneous quantification of 4 key enzymes whose expression is down-regulated in different mitochondrial diseases: Complex I, Complex IV, PDH and Frataxin. The array is comprised of 4 monoclonal capture antibodies striped onto a dipstick, and the immunocaptured sample is detected using 4 monoclonal detector antibodies that recognize different epitopes on the target enzymes. This array is very rapid (total assay time including sample prep is less than 1 hour) and very sensitive, and as with all Abcam's assays, isolation of mitochondria is not required.
This array is suitable for testing a variety of sample types. Not only is it useful for measuring samples that are deficient in the 4 enzymes due to genetic mutations, it is also useful for measuring changes in the expression of the enzymes due to drug treatments or other manipulations.
Notes
All components are stable in their provided containers at room temperature out of direct sunlight.
After diluting the 10X Blocking Buffer to 2X, store at 4°C.
For long-term storage, all buffers can be stored at 4°C.
After diluting the 10X Blocking Buffer to 2X, store at 4°C.
For long-term storage, all buffers can be stored at 4°C.
Tested applications
Sandwich ELISAmore details
Properties
Storage instructions
Please see Notes section
| Components | 90 tests | 30 tests |
|---|---|---|
| Buffer A (Extraction buffer) | 1 x 45ml | 1 x 15ml |
| Buffer B (10X Blocking solution) | 1 x 1.2ml | 1 x 0.4ml |
| Buffer C (Wash buffer) | 6 x 1ml | 2 x 1ml |
| Dipsticks | 1 x 90 units | 1 x 30 units |
| Gold-conjugated antibody (dried in microplate wells) | 1 x 90 tests | 1 x 30 tests |
Applications
Show applications key
Our Abpromise guarantee covers the use of ab109879 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Sandwich ELISA
sELISAsELISA
MetaPath™ Mito Disease 4-Plex Dipstick Array images:
ELISA - MetaPath™ Mito Disease 4-Plex Dipstick Array (ab109879)
A typical standard curve for ab109879 using HepG2 cell extract. We suggest using 7 to 8 dipsticks for covering the working range. In this example, the maximum sample load should be 5 µg linear regression above which non-linear regression should be used.
ELISA - MetaPath™ Mito Disease 4-Plex Dipstick Array (ab109879)
Examples of dipsticks from (a) normal fibroblast cells (b) rho0 fibroblast cells which lack mtDNA and hence complexes I and IV only (c) a fibroblast sample from a patient exhibiting a specific deficiency in complex I (d) pure frataxin and (e) buffer control with containing no sample. Data from these sticks was collected using a simple flatbed scanner and ImageJ software.
Sandwich ELISA - MetaPath™ Mito Disease 4-Plex Dipstick Array (ab109879)
Dipstick assays use the well-established lateral flow concept, whereby capture antibodies are striped onto nitrocellulose membrane and a Whatman paper wicking pad draws the sample through the antibody bands. Detector antibodies, conjugated to gold, are dried in the wells of a 96-well plate. Sample is added to the well, the dipstick inserted, and within minutes the line for each target is revealed as the protein-detector antibody-gold complex binds with the capture antibodies. Multiplexing dipstick assays have multiple target protein lines. A positive control goat anti-mouse antibody line is included on all assays to ensure that adequate wicking of the sample occurred.
Protocols
References for MetaPath™ Mito Disease 4-Plex Dipstick Array (ab109879)
This product has been referenced in:
- Ichikawa Y et al. Disruption of ATP-binding cassette B8 in mice leads to cardiomyopathy through a decrease in mitochondrial iron export. Proc Natl Acad Sci U S A 109:4152-7 (2012).Read more (PubMed: 22375032) »
See 1 publication for this product
Publishing research using ab109879? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"