Anti-Methylated Lysine antibody (FITC) (ab53527)

Overview

  • Product nameAnti-Methylated Lysine antibody (FITC)
    See all Methylated Lysine primary antibodies
  • Description
    Rabbit polyclonal to Methylated Lysine (FITC)
  • ConjugationFITC. Ex: 493nm, Em: 528nm
  • Conjugation notesFITC/Ab molar ratio: approximately 10:1
  • SpecificityThis antibody recognizes proteins methylated on lysine residues (mono and di-methylysine). It does not cross-react with acetylated proteins. Cross-reactivity to trimethyllysine has not been tested.
  • Tested applicationsICC/IFmore details
  • Species reactivity
    Reacts with: Species independent
  • Immunogen

    Methylated protein [s] conjugated to KLH.

  • Positive control
    • Human melanoma cells (MMRU).

Properties

Applications

Our Abpromise guarantee covers the use of ab53527 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/50 - 1/100.

Target

  • RelevanceMany proteins are post translationally modified. Modifications such as phosphorylation, glycosylation, ubiquitination and methylation have been shown to play an important role in the development, physiology and disease of animals and plants. Histone lysine methylation is regarded as a very stable modification with important functions in epigenetic gene control and for organizing chromatin domains.
  • Alternative names
    • Dimethylysine antibody
    • Methyl lysine antibody
    • N epsilon dimethyl lysine antibody
    • N epsilon mono methyllysine antibody
    • N epsilon trimethyl lysine antibody
    • N(epsilon) trimethyl lysine antibody
    • Trimethyl lysine antibody
    see all

Anti-Methylated Lysine antibody (FITC) images

  • This image shows human melanoma (MMRU) cells stained with ab53527 at 1/50 dilution.

References for Anti-Methylated Lysine antibody (FITC) (ab53527)

ab53527 has not yet been referenced specifically in any publications.

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