Products:Epigenetics and Nuclear Signaling >> Transcription >> Domain Families >> HLH / Leucine Zipper
If your product does not perform as described on this datasheet, we will refund or replace your product...
Read our guarantee »Publishing research using ab13703? Please let us know so that we can cite the reference in this datasheet
ab13703 has been referenced in 4 publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Predicted band size : 59 kDa
Western blot using MiTF antibody [21D1418] (ab13703)
Lane 1: A375 cell lysate (human) using 1:1000 dilution
Lane 2: 10 µg of Raw cell lysates (mouse) using 1:250 dilution
Secondary: HRP-conjugated anti mouse antibody, developed using the ECL technique
Overlay histogram showing Malme-3 cells stained with ab13703 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab13703, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (
0
Call 01223 696 000 or contact us