MiTF antibody [21D1418] (ab13703)

Product Name 

MiTF antibody [21D1418]

Product type 

Primary antibodies

Description 

Mouse monoclonal [21D1418] to MiTF

Immunogen 

Synthetic peptide: SEARALAKERQK, corresponding to amino acids 301-312 of Human MiTF.
BLAST 'SEARALAKERQK' with or

Reacts with 

Hu, Ms

Predicted to react 
(species key)

Rat, Chk, Cow, Dog, Hrs, Ql, Xl
(Due to sequence homology)

Tested applications 
(see key)

WB

Abreviews 

Customer reviews (see key)

Read about Abreviews

Application notes 
(see key)

Recommended dilutions
WB: 1/250 - 1/1000. Detects a band of approximately 55 kDa (predicted molecular weight: 59 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

A375 (human) and Raw (mouse) cells.

Cellular localization 

Nuclear

Research areas 

Immunology >> Innate Immunity >> Mast Cells
Chromatin and Nuclear Signaling >> Transcription >> Domain Families >> HLH / Leucine Zipper >> HLH / Leucine Zipper

Relevance 

MiTF (Microphthalmia associated transcription factor) is a basic helix loop helix leucine zipper (b HLH ZIP) transcription factor implicated in pigmentation, mast cells and bone development. Mutations in MiTF cause auditory pigmentary syndromes, such as Waardenburg syndrome type II, type IIa and Tietz syndrome in humans. There are two known isoforms of MiTF differing by 66 amino acids at the NH2 terminus. Shorter forms are expressed in melanocytes and run as two bands at 52kDa and 56kDa, while the longer Mi form runs as a cluster of bands at 60-70kDa in osteoclasts and in B16 melonoma cells (but not other melanoma cell lines), as well as mast cells and heart. MiTF plays a critical role in the differentiation of various cell types as neural crest-derived melanocytes, mast cells, osteoclasts and optic cup-derived retinal pigment epithelium.

Database links 

Raised in 

Mouse

Clonality 

Monoclonal

Clone number 

21D1418

Isotype 

IgG1

Light chain type 

kappa

Purity 

Protein G purified

Storage buffer 

Preservative: 0.02% Sodium Azide
Constituents: PBS

Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS

Form 

Liquid

Concentration 

0.500 mg/ml

Storage instructions 

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

See below for MiTF antibody [21D1418] images, references, products related to ab13703 and other tools.

MiTF antibody [21D1418] images:

References for MiTF antibody [21D1418] (ab13703)

  This product has been used in: (two most recent references)

Lee J et al. Mechanisms of melanogenesis inhibition by 2,5-dimethyl-4-hydroxy-3(2H)-furanone. Br J Dermatol 157:242-8 (2007). PubMed: 17650175

Huan C et al. Transcription factors TFE3 and TFEB are critical for CD40 ligand expression and thymus-dependent humoral immunity. Nat Immunol 7:1082-91 (2006). PubMed: 16936731
If you publish research using ab13703 please let us know so that we can cite the reference on this datasheet.

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