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Anti-MiTF antibody [21D1418] (ab13703)

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Overview

Product name

Anti-MiTF antibody [21D1418]
See all MiTF products (10) ...

Description

Mouse monoclonal [21D1418] to MiTF

Tested applications

WB, Flow Cytmore details

Cross reactivity

Reacts with

Mouse, Human

Predicted to work with

Rat, Horse, Chicken, Cow, Dog, Xenopus laevis, Quail

Immunogen

Synthetic peptide: SEARALAKERQK, corresponding to amino acids 301-312 of Human MiTF.

SEARALAKER QK

Positive control

A375 (human) and Raw (mouse) cells.

Properties

Form

Liquid

Storage instructions

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Storage buffer

Preservative: 0.02% Sodium Azide
Constituents: PBS

Concentration

Concentration information loading...

Purity

Protein G purified

Clonality

Monoclonal

Clone number

21D1418

Isotype

IgG1

Light chain type

kappa

  • Western blot - MiTF antibody [21D1418] (ab13703)Western blot - MiTF antibody [21D1418] (ab13703) image (enlarge)

  • Flow Cytometry-Anti-MiTF antibody [21D1418](ab13703)Flow Cytometry-Anti-MiTF antibody [21D1418](ab13703) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab13703 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Target

Function

Transcription factor for tyrosinase and tyrosinase-related protein 1. Binds to a symmetrical DNA sequence (E-boxes) (5'-CACGTG-3') found in the tyrosinase promoter. Plays a critical role in the differentiation of various cell types as neural crest-derived melanocytes, mast cells, osteoclasts and optic cup-derived retinal pigment epithelium.

Tissue specificity

Isoform M is exclusively expressed in melanocytes and melanoma cells. Isoform A and isoform H are widely expressed in many cell types including melanocytes and retinal pigment epithelium (RPE). Isoform C is expressed in many cell types including RPE but not in melanocyte-lineage cells.

Involvement in disease

Defects in MITF are the cause of Waardenburg syndrome type 2A (WS2A) [MIM:193510]. It is a dominant inherited disorder characterized by sensorineural hearing loss and patches of depigmentation. The features show variable expression and penetrance.
Defects in MITF are a cause of Waardenburg syndrome type 2 with ocular albinism (WS2-OA) [MIM:103470]. It is an ocular albinism with sensorineural deafness.
Defects in MITF are the cause of Tietz syndrome (TIETZS) [MIM:103500]. It is an autosomal dominant disorder characterized by generalized hypopigmentation and profound, congenital, bilateral deafness. Penetrance is complete.

Sequence similarities

Belongs to the MiT/TFE family.
Contains 1 basic helix-loop-helix (bHLH) domain.

Post-translational
modifications

Phosphorylation at Ser-405 significantly enhances the ability to bind the tyrosinase promoter.

Cellular localization

Nucleus.

Target information above from: UniProt accessionO75030 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • BHLHE32 antibody
  • bHLHe32 antibody
  • Class E basic helix-loop-helix protein 32 antibody
  • Homolog of mouse microphthalmia antibody
  • Mi antibody
  • Microphthalmia associated transcription factor antibody
  • Microphthalmia-associated transcription factor antibody
  • MITF antibody
  • MITF_HUMAN antibody
  • WS2 antibody
  • WS2A antibody
see all

Anti-MiTF antibody [21D1418] images:

  Western blot - MiTF antibody [21D1418] (ab13703)

Western blot - MiTF antibody [21D1418] (ab13703)



Predicted band size : 59 kDa


Western blot using MiTF antibody [21D1418] (ab13703)

Lane 1: A375 cell lysate (human) using 1:1000 dilution
Lane 2: 10 µg of Raw cell lysates (mouse) using 1:250 dilution

Secondary: HRP-conjugated anti mouse antibody, developed using the ECL technique

  Flow Cytometry-Anti-MiTF antibody [21D1418](ab13703)

Flow Cytometry-Anti-MiTF antibody [21D1418](ab13703)

Overlay histogram showing Malme-3 cells stained with ab13703 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab13703, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells ) used under the same conditions. Acquisition of >5,000 events was performed.

References for Anti-MiTF antibody [21D1418] (ab13703)

This product has been referenced in:

  • Liang Het al. CtBP2 Downregulation during Neural Crest Specification Induces Expression of Mitf and REST, Resulting in Melanocyte Differentiation and Sympathoadrenal Lineage Suppression. Mol Cell Biol 31:955-70 (2011). WB; Quail.Read more (PubMed: 21199918) »
  • Shimoda Net al. Effect of heterocyclic pyrimidine compounds on UVB-induced cell damage in human keratinocytes and on melanogenesis in mouse B16 cells. Biol Pharm Bull 33:862-8 (2010). WB; Mouse.Read more (PubMed: 20460767) »

See all 4 publications for this product

Publishing research using ab13703? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"