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Western blot - MiTF antibody [21D1418] (ab13703)
Western blot - MiTF antibody [21D1418] (ab13703)
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Product Name 

MiTF antibody [21D1418]

 

See all MiTF antibodies (11)...

Product type 

Primary antibodies

Description 

Mouse monoclonal [21D1418] to MiTF

Immunogen 

Synthetic peptide: SEARALAKERQK, corresponding to amino acids 301-312 of Human MiTF.
BLAST 'SEARALAKERQK' with BLAST the sequence with ExPASy or BLAST the sequence with NCBI

Reacts with 

Hu, Ms

Predicted to react 
(species key)

Rat, Chk, Cow, Dog, Hrs, Ql, Xl
(Due to sequence homology)

Tested applications 
(see key)

WB


Abreviews 

Customer reviews
(see key)


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Application notes 
(see key)

Recommended dilutions
WB: 1/250 - 1/1000. Detects a band of approximately 55 kDa (predicted molecular weight: 59 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

A375 (human) and Raw (mouse) cells.

Cellular localization 

Nuclear

Research areas 

Immunology >> Innate Immunity >> Mast Cells
Chromatin and Nuclear Signaling >> Transcription >> Domain Families >> HLH / Leucine Zipper >> HLH / Leucine Zipper

Relevance 

MiTF (Microphthalmia associated transcription factor) is a basic helix loop helix leucine zipper (b HLH ZIP) transcription factor implicated in pigmentation, mast cells and bone development. Mutations in MiTF cause auditory pigmentary syndromes, such as Waardenburg syndrome type II, type IIa and Tietz syndrome in humans. There are two known isoforms of MiTF differing by 66 amino acids at the NH2 terminus. Shorter forms are expressed in melanocytes and run as two bands at 52kDa and 56kDa, while the longer Mi form runs as a cluster of bands at 60-70kDa in osteoclasts and in B16 melonoma cells (but not other melanoma cell lines), as well as mast cells and heart. MiTF plays a critical role in the differentiation of various cell types as neural crest-derived melanocytes, mast cells, osteoclasts and optic cup-derived retinal pigment epithelium.

 

Alternative names MiTF antibody [21D1418] (ab13703)

Database links 

The links below go to external sites and will open in a new browser window

Entrez Gene

    

407219    (Cow)
415126    (Dog)
4286    (Human)
17342    (Mouse)
25094    (Rat)

Omim

    

156845    (Human)

SwissProt

    

A1E097    (Dog)
O75030    (Human)
Q08874    (Mouse)
O88368    (Rat)

Raised in 

Mouse

Clonality 

Monoclonal

Clone number 

21D1418

Isotype 

IgG1

Light chain type 

kappa

Purity 

Protein G purified

Storage buffer 

Preservative: 0.02% Sodium Azide
Constituents: PBS

Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS

Form 

Liquid

Concentration 

0.500 mg/ml

Storage instructions 

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

 

At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this MiTF antibody [21D1418] is on this datasheet. But please do contact us if you would like any reassurance!


See below for MiTF antibody [21D1418] images, references, products related to ab13703 and other tools.

 

MiTF antibody [21D1418] images:

Product image  Western blot - MiTF antibody [21D1418] (ab13703)

Western blot - MiTF antibody [21D1418] (ab13703)



Predicted band size : 59 kDa


Western blot using MiTF antibody [21D1418] (ab13703)

Lane 1: A375 cell lysate (human) using 1:1000 dilution
Lane 2: 10 µg of Raw cell lysates (mouse) using 1:250 dilution

Secondary: HRP-conjugated anti mouse antibody, developed using the ECL technique

 

References for MiTF antibody [21D1418] (ab13703)

Specific publication references   This product has been used in: (two most recent references)

Lee J et al. Mechanisms of melanogenesis inhibition by 2,5-dimethyl-4-hydroxy-3(2H)-furanone. Br J Dermatol 157:242-8 (2007). PubMed: 17650175

Huan C et al. Transcription factors TFE3 and TFEB are critical for CD40 ligand expression and thymus-dependent humoral immunity. Nat Immunol 7:1082-91 (2006). PubMed: 16936731
If you publish research using ab13703 please let us know so that we can cite the reference on this datasheet.

 

Search PubMed (MEDLINE) for references to MiTF

 

MiTF antibody [21D1418] - more information

Customer reviews (feedback) regarding MiTF antibody [21D1418]

Customer FAQs regarding MiTF antibody [21D1418]

Protocols for MiTF antibody [21D1418]

Price and availability of products related to MiTF antibody [21D1418]

 

MiTF antibody [21D1418] - related products:

Products for Western blot

Loading controls

Lysates

 

Positive Controls for MiTF antibody [21D1418]

ab7187

  

RAW 264.7 (Mouse leukaemic monocyte macrophage cell line) Whole Cell Lysate

 

Compatible Secondaries for MiTF antibody [21D1418]

ab2891

  

HRP polymer antibody

ab6563

  

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ab6726

  

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Alkaline Phosphatase antibody

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Phycoerythrin antibody

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Agarose antibody

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15nm Gold antibody

ab27230

  

10nm Gold antibody

ab27231

  

15nm Gold antibody

ab27233

  

5nm Gold antibody

ab27241

  

10nm Gold antibody

ab27242

  

20nm Gold antibody

ab27243

  

40nm Gold antibody

ab27244

  

5nm Gold antibody

ab60313

  

Chromeo™ 488 antibody

ab60316

  

Chromeo™ 546 antibody

ab60318

  

Chromeo™ 642 antibody

 

Other MiTF Antibodies

See all MiTF antibodies (11)...

 

MiTF antibody [21D1418] - other tools:

Contact Abcam with a Technical Enquiry about MiTF antibody [21D1418]

 

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