![Western blot - MiTF antibody [21D1418] (ab13703) image](#ab13703_1.jpg)
 image](#MiTF-Primary-antibodies-ab13703-1.jpg)
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Products:Epigenetics and Nuclear Signaling >> Transcription >> Domain Families >> HLH / Leucine Zipper
Overview
Product name
Anti-MiTF antibody [21D1418]
See all MiTF products (10) ...
Description
Mouse monoclonal [21D1418] to MiTF
Tested applications
WB, Flow Cytmore details
Cross reactivity
Reacts with
Mouse, Human
Predicted to work with
Rat, Horse, Chicken, Cow, Dog, Xenopus laevis, Quail
Immunogen
Synthetic peptide: SEARALAKERQK, corresponding to amino acids 301-312 of Human MiTF.
SEARALAKER QK
Positive control
A375 (human) and Raw (mouse) cells.
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: PBS
Concentration
Concentration information loading...
Purity
Protein G purified
Clonality
Monoclonal
Clone number
21D1418
Isotype
IgG1
Light chain type
kappa
Applications
Show applications key
Our Abpromise guarantee covers the use of ab13703 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/250 - 1/1000.Detects a band of approximately 55 kDa (predicted molecular weight: 59 kDa).
Flow Cyt: Use 1µg for 106 cells.
Target
Function
Transcription factor for tyrosinase and tyrosinase-related protein 1. Binds to a symmetrical DNA sequence (E-boxes) (5'-CACGTG-3') found in the tyrosinase promoter. Plays a critical role in the differentiation of various cell types as neural crest-derived melanocytes, mast cells, osteoclasts and optic cup-derived retinal pigment epithelium.
Tissue specificity
Isoform M is exclusively expressed in melanocytes and melanoma cells. Isoform A and isoform H are widely expressed in many cell types including melanocytes and retinal pigment epithelium (RPE). Isoform C is expressed in many cell types including RPE but not in melanocyte-lineage cells.
Involvement in disease
Defects in MITF are the cause of Waardenburg syndrome type 2A (WS2A) [MIM:193510]. It is a dominant inherited disorder characterized by sensorineural hearing loss and patches of depigmentation. The features show variable expression and penetrance.
Defects in MITF are a cause of Waardenburg syndrome type 2 with ocular albinism (WS2-OA) [MIM:103470]. It is an ocular albinism with sensorineural deafness.
Defects in MITF are the cause of Tietz syndrome (TIETZS) [MIM:103500]. It is an autosomal dominant disorder characterized by generalized hypopigmentation and profound, congenital, bilateral deafness. Penetrance is complete.
Sequence similarities
Belongs to the MiT/TFE family.
Contains 1 basic helix-loop-helix (bHLH) domain.
Post-translational
modifications
Phosphorylation at Ser-405 significantly enhances the ability to bind the tyrosinase promoter.
Cellular localization
Nucleus.
Target information above from: UniProt accessionO75030
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- BHLHE32 antibody
- bHLHe32 antibody
- Class E basic helix-loop-helix protein 32 antibody
see all
Database links
- Entrez Gene: 395886 Chicken
- Entrez Gene: 407219 Cow
- Entrez Gene: 4286 Human
- Entrez Gene: 17342 Mouse
- Entrez Gene: 25094 Rat
- Omim: 156845 Human
- SwissProt: A1E097 Dog
- SwissProt: O75030 Human
see all
Anti-MiTF antibody [21D1418] images:
Western blot - MiTF antibody [21D1418] (ab13703)
![Western blot - MiTF antibody [21D1418] (ab13703)](/ps/datasheet/Images/13/ab13703/ab13703_1.jpg)
Predicted band size : 59 kDa
Western blot using MiTF antibody [21D1418] (ab13703)
Lane 1: A375 cell lysate (human) using 1:1000 dilution
Lane 2: 10 µg of Raw cell lysates (mouse) using 1:250 dilution
Secondary: HRP-conjugated anti mouse antibody, developed using the ECL technique
Flow Cytometry-Anti-MiTF antibody [21D1418](ab13703)
](/ps/datasheet/images/13/ab13703/MiTF-Primary-antibodies-ab13703-1.jpg)
Overlay histogram showing Malme-3 cells stained with ab13703 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab13703, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells ) used under the same conditions. Acquisition of >5,000 events was performed.
References for Anti-MiTF antibody [21D1418] (ab13703)
This product has been referenced in:
- Liang Het al. CtBP2 Downregulation during Neural Crest Specification Induces Expression of Mitf and REST, Resulting in Melanocyte Differentiation and Sympathoadrenal Lineage Suppression. Mol Cell Biol 31:955-70 (2011). WB; Quail.Read more (PubMed: 21199918) »
- Shimoda Net al. Effect of heterocyclic pyrimidine compounds on UVB-induced cell damage in human keratinocytes and on melanogenesis in mouse B16 cells. Biol Pharm Bull 33:862-8 (2010). WB; Mouse.Read more (PubMed: 20460767) »
See all 4 publications for this product
Publishing research using ab13703? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"