![Western blot - MiTF antibody [C5] (ab12039) image](#MiTF-Primary-antibodies-ab12039-8.jpg)
![Immunohistochemistry (Frozen sections) - Anti-MiTF antibody [C5] (ab12039) image](#MiTF-Primary-antibodies-ab12039-30.jpg)
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Products:Epigenetics and Nuclear Signaling >> Transcription >> Domain Families >> HLH / Leucine Zipper
Overview
Product name
Anti-MiTF antibody [C5]
See all MiTF products (10) ...
Description
Mouse monoclonal [C5] to MiTF
Specificity
The antibody recognizes serine phosphorylated and non-phosphorylated melanocytic isoforms of microphthalmia.
Tested applications
IHC-Fr, IHC-P, ICC/IF, ICC, WB, IP, Gel supershift assaysmore details
Cross reactivity
Reacts with
Mouse, Rat, Human
Immunogen
N-terminal fragment of human microphthalmia protein and mouse myeloma NS1 cells.
Positive control
This antibody gave a positive signal in Malme 3M whole cell lysate.
General notes
Do not store in a frost-free freezer.
The antibody is stable for at least 12 months when stored appropriately. Working dilutions should be discarded if not used within 12 hours.
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: PBS, pH 7.4
Concentration
Concentration information loading...
Purity
IgG fraction
Clonality
Monoclonal
Clone number
C5
Isotype
IgG1
Research areas
Immunology >> Innate Immunity >> Mast Cells
Epigenetics and Nuclear Signaling >> Transcription >> Domain Families >> HLH / Leucine Zipper >> HLH / Leucine Zipper
Epigenetics and Nuclear Signaling >> Transcription >> Domain Families >> HLH / Leucine Zipper >> HLH
Epigenetics and Nuclear Signaling >> Transcription >> Domain Families >> HLH / Leucine Zipper
Applications
Show applications key
Our Abpromise guarantee covers the use of ab12039 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-Fr: Use at an assay dependent concentration.
IHC-P: Use a concentration of 1 µg/mlAntigen retrieval is not essential but may optimise staining.
ICC/IF: Use at an assay dependent dilution. (Fix with methanol/acetone, block with 1% BSA/PBS (see Wellbrock et al).)
ICC: Use at an assay dependent dilution. (Fix with methanol/acetone, block with 1% BSA/PBS (see Wellbrock et al).)
WB: Use a concentration of 1 µg/mlDetects a band of approximately 52-56 kDa (predicted molecular weight: 59 kDa).
IP: Use at 2 µg/mg of lysate.
GSA: Use at an assay dependent dilution.
Target
Function
Transcription factor for tyrosinase and tyrosinase-related protein 1. Binds to a symmetrical DNA sequence (E-boxes) (5'-CACGTG-3') found in the tyrosinase promoter. Plays a critical role in the differentiation of various cell types as neural crest-derived melanocytes, mast cells, osteoclasts and optic cup-derived retinal pigment epithelium.
Tissue specificity
Isoform M is exclusively expressed in melanocytes and melanoma cells. Isoform A and isoform H are widely expressed in many cell types including melanocytes and retinal pigment epithelium (RPE). Isoform C is expressed in many cell types including RPE but not in melanocyte-lineage cells.
Involvement in disease
Defects in MITF are the cause of Waardenburg syndrome type 2A (WS2A) [MIM:193510]. It is a dominant inherited disorder characterized by sensorineural hearing loss and patches of depigmentation. The features show variable expression and penetrance.
Defects in MITF are a cause of Waardenburg syndrome type 2 with ocular albinism (WS2-OA) [MIM:103470]. It is an ocular albinism with sensorineural deafness.
Defects in MITF are the cause of Tietz syndrome (TIETZS) [MIM:103500]. It is an autosomal dominant disorder characterized by generalized hypopigmentation and profound, congenital, bilateral deafness. Penetrance is complete.
Sequence similarities
Belongs to the MiT/TFE family.
Contains 1 basic helix-loop-helix (bHLH) domain.
Post-translational
modifications
Phosphorylation at Ser-405 significantly enhances the ability to bind the tyrosinase promoter.
Cellular localization
Nucleus.
Target information above from: UniProt accessionO75030
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- BHLHE32 antibody
- bHLHe32 antibody
- Class E basic helix-loop-helix protein 32 antibody
see all
Database links
- Entrez Gene: 4286 Human
- Entrez Gene: 17342 Mouse
- Entrez Gene: 25094 Rat
- Omim: 156845 Human
- SwissProt: O75030 Human
- SwissProt: Q08874 Mouse
- SwissProt: O88368 Rat
- Unigene: 166017 Human
see all
Anti-MiTF antibody [C5] images:
Western blot - MiTF antibody [C5] (ab12039)
![Western blot - MiTF antibody [C5] (ab12039)](/ps/datasheet/images/12/ab12039/MiTF-Primary-antibodies-ab12039-8.jpg)
Anti-MiTF antibody [C5] (ab12039) at 1 µg/ml + Malme 3m (Human melanoma cells) Whole Cell Lysate at 10 µg
Secondary
Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 59 kDa
Observed band size : 40,55,70 kDa (why is the actual band size different from the predicted?)
Exposure time : 20 minutes
Immunohistochemistry (Frozen sections) - Anti-MiTF antibody [C5] (ab12039)
![Immunohistochemistry (Frozen sections) - Anti-MiTF antibody [C5] (ab12039)](/ps/datasheet/images/12/ab12039/MiTF-Primary-antibodies-ab12039-30.jpg)
ab12039 staining MiTF in embryonic day 4 chick eye tissue by Immunohistochemistry (Frozen sections).Tissue was fixed with 4% paraformaldehyde, permeabilized using 1% saponin, blocked with 10% serum for 30 minutes at 37°C, then incubated with ab12039 at a 1/1000 dilution for 24 hours at 4deg;C. The secondary used was an Alexa-Fluor 488 conjugated mouse monoclonal, used at a 1/100 dilution. Counterstained with DAPI (blue).
Image courtesy of Katia Del Rio-Tsomis by Abreview.
References for Anti-MiTF antibody [C5] (ab12039)
This product has been referenced in:
- Terragni Jet al. The E-box Binding Factors Max/Mnt, MITF, and USF1 Act Coordinately with FoxO to Regulate Expression of Proapoptotic and Cell Cycle Control Genes by Phosphatidylinositol 3-Kinase/Akt/Glycogen Synthase Kinase 3 Signaling. J Biol Chem 286:36215-27 (2011). WB; Human.Read more (PubMed: 21873430) »
- Maresca Vet al. MC1R stimulation by alpha-MSH induces catalase and promotes its re-distribution to the cell periphery and dendrites. Pigment Cell Melanoma Res : (2010). WB; Mouse.Read more (PubMed: 20067588) »
See all 13 publications for this product
Publishing research using ab12039? Please let us know so that we can cite the reference in this datasheet
![MiTF antibody [C5] for Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) in Human (12039)](/ps/datasheet/images/12/ab12039/MiTF-Primary-antibodies-ab12039-11.jpg)
![Anti-MiTF antibody [C5] for Immunohistochemistry (Frozen sections) in Chicken (12039)](/ps/datasheet/images/12/ab12039/MiTF-Primary-antibodies-ab12039-28.JPG)
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"