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Product Name
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MiTF antibody [D5]
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See all MiTF antibodies (11)...
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Product type
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Primary antibodies
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Description
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Mouse monoclonal [D5] to MiTF
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Immunogen
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N-terminal fragment of human Mi protein.
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Reacts with
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Hu, Dog
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Does not react with
(species key)
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Ms, Rat
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Specificity
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ab3201 does not cross-react with other b-HLH-ZIP factors by DNA mobility shift assay.
It reacts with both melanocytic and non-melanocytic isoforms of Mi. It is especially useful for research on murine Mi protein.
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Epitope
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N-terminal
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Tested applications
(see key)
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GSA, IHC-P, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
GSA: Use at a concentration of 1 mg/ml.
IHC-P: Use at an assay dependent dilution.
WB: Use at an assay dependent dilution. Predicted molecular weight: 59 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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501 Mel cells
Melanoma
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Cellular localization
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Nuclear
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Research areas
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Immunology >> Innate Immunity >> Mast Cells
Chromatin and Nuclear Signaling >> Transcription >> Domain Families >> HLH / Leucine Zipper >> HLH / Leucine Zipper
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Relevance
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MiTF (Microphthalmia associated transcription factor) is a basic helix loop helix leucine zipper (b HLH ZIP) transcription factor implicated in pigmentation, mast cells and bone development. Mutations in MiTF cause auditory pigmentary syndromes, such as Waardenburg syndrome type II, type IIa and Tietz syndrome in humans. There are two known isoforms of MiTF differing by 66 amino acids at the NH2 terminus. Shorter forms are expressed in melanocytes and run as two bands at 52kDa and 56kDa, while the longer Mi form runs as a cluster of bands at 60-70kDa in osteoclasts and in B16 melonoma cells (but not other melanoma cell lines), as well as mast cells and heart. MiTF plays a critical role in the differentiation of various cell types as neural crest-derived melanocytes, mast cells, osteoclasts and optic cup-derived retinal pigment epithelium.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Mouse
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Clonality
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Monoclonal
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Clone number
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D5
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Isotype
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IgG1
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Light chain type
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kappa
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Purity
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Immunogen affinity purified
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Storage buffer
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10mM PBS, pH7.4, 0.2%BSA, 0.09% sodium azide Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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0.200 mg/ml
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Storage instructions
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Store at +4°C. Do not freeze.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this MiTF antibody [D5] is on this datasheet. But please do contact us if you would like any reassurance! |
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References for MiTF antibody [D5] (ab3201)
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This product has been used in:
Petersen-Jones SM et al. Ocular melanosis in the Cairn Terrier: histopathological description of the condition, and immunohistological and ultrastructural characterization of the characteristic pigment-laden cells. Vet Ophthalmol 11:260-8 (2008). IHC-P; Dog. PubMed: 18638353
If you publish research using ab3201 please let us know so that we can cite the reference on this datasheet.
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Search PubMed (MEDLINE) for references to MiTF
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MiTF antibody [D5] - more information
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Customer reviews (feedback) regarding MiTF antibody [D5] |
Customer FAQs regarding MiTF antibody [D5] |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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