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Western blot - MiTF antibody (ab20663)
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Western blot - MiTF antibody (ab20663)
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - MiTF antibody (ab20663)
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Product Name
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MiTF antibody
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See all MiTF antibodies (11)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to MiTF
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Immunogen
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Synthetic peptide conjugated to KLH derived from within residues 500 to the C-terminus of Human MiTF.
(Peptide available as ab21454.)
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Reacts with
(species key)
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Hu, Ms, Rat
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Tested applications
(see key)
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IHC-P, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
IHC-P: 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol (See Abreview).
WB: Use at a concentration of 1 µg/ml. Detects a band of approximately 52 kDa (predicted molecular weight: 52 kDa). Can be blocked with MiTF peptide (ab21454).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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This antibody gave a positive signal in the following whole cell lysates:
HeLa (Human epithelial carcinoma cell line)
A431 (Human epithelial carcinoma cell line)
NIH 3T3 (Mouse embryonic fibroblast cell line)
MEF1 (Mouse embryonic fibroblast cell line)
PC12 (Rat adrenal pheochromocytoma cell line)
This antibody gave a positive signal in HeLa nuclear lysate
This antibody gave a positive signal in the following tissue lysates:
Brain (Mouse and Rat)
Testis (Mouse)
Spinal Cord (Mouse)
Ovary (Mouse)
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Cellular localization
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Nuclear
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Research areas
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Immunology >> Innate Immunity >> Mast Cells
Chromatin and Nuclear Signaling >> Transcription >> Domain Families >> HLH / Leucine Zipper >> HLH / Leucine Zipper
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Relevance
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MiTF (Microphthalmia associated transcription factor) is a basic helix loop helix leucine zipper (b HLH ZIP) transcription factor implicated in pigmentation, mast cells and bone development. Mutations in MiTF cause auditory pigmentary syndromes, such as Waardenburg syndrome type II, type IIa and Tietz syndrome in humans. There are two known isoforms of MiTF differing by 66 amino acids at the NH2 terminus. Shorter forms are expressed in melanocytes and run as two bands at 52kDa and 56kDa, while the longer Mi form runs as a cluster of bands at 60-70kDa in osteoclasts and in B16 melonoma cells (but not other melanoma cell lines), as well as mast cells and heart. MiTF plays a critical role in the differentiation of various cell types as neural crest-derived melanocytes, mast cells, osteoclasts and optic cup-derived retinal pigment epithelium.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4 Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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Batch dependent within range: 0.60 - 0.60 mg/ml
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Storage instructions
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Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this MiTF antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for MiTF antibody images, references, products related to ab20663 and other tools.
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MiTF antibody images:
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 Western blot - MiTF antibody (ab20663)
All lanes : MiTF antibody (ab20663) at 1 µg/ml
Lane 1 : HeLa whole cell lysate
Lane 2 : HeLa nuclear lysate
Lane 3 : A431 whole cell lysate
Lane 4 : HeLa whole cell lysate with MiTF peptide (ab21454) at 1 µg/ml
Lane 5 : HeLa nuclear lysate with MiTF peptide (ab21454) at 1 µg/ml
Lane 6 : A431 whole cell lysate with MiTF peptide (ab21454) at 1 µg/ml
Lysates/proteins at 20 µg per lane.
Secondary
Alexa Fluor Goat polyclonal to Rabbit IgG (700) at 1/10000 dilution
Predicted band size : 52 kDa
Observed band size : 52 kDa
Additional bands at : 25 kDa,37 kDa. We are unsure as to the identity of these extra bands.
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Western blot - MiTF antibody (ab20663)
All lanes : MiTF antibody (ab20663) at 1 µg/ml
Lane 1 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate (ab7179)
Lane 2 : MEF1 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
Lane 3 : Brain (Mouse) Tissue Lysate
Lane 4 : Testis (Mouse) Tissue Lysate - normal tissue
Lane 5 : Spinal Cord (Mouse) Tissue Lysate
Lane 6 : Ovary (Mouse) Tissue Lysate - normal tissue
Lane 7 : PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate
Lane 8 : Brain (Rat) Tissue Lysate - normal tissue
Lysates/proteins at 10 µg per lane.
Secondary
IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size : 52 kDa
Observed band size : 52 kDa
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - MiTF antibody (ab20663)
ab20663 staining MiTF in mouse skin tissue section by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Tissue section was fixed in formaldehyde and after heat mediated antigen retrieval, blocked (5 minutes/peroxidase block then 10 minutes/protein block) for 15 minutes at 20°C. The primary antibody was diluted, 1/250 and incubated with sample for 45 minutes at 20°C. A HRP conjugated goat polyclonal to rabbit IgG was used undiluted, as the secondary. This image is courtesy of an Abreview submitted by Antibody Solutions Ltd.
See Abreview
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - MiTF antibody (ab20663)
ab20663 staining MiTF in meanoma tissue sections by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Tissue underwent formaldehyde fixation before heat mediated antigen retrieval and then blocked (5 minutes of peroxidase block and 10 minutes of protein block) for 15 minutes at 20°C. The primary antibody was used undiluted and incubated with sample for 45 minutes at 20°C. A HRP conjugated goat polyclonal to rabbit IgG was used, undiluted as secondary. This image is a courtesy of Antibody Solutions Ltd.
See Abreview
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Search PubMed (MEDLINE) for references to MiTF
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MiTF antibody - more information
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Customer reviews (feedback) regarding MiTF antibody |
Customer FAQs regarding MiTF antibody |
Protocols for MiTF antibody |
Price and availability of products related to MiTF antibody |
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MiTF antibody - related products:
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MiTF antibody - other tools:
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Contact Abcam with a Technical Enquiry about MiTF antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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