Anti-MiTF antibody (ab73930)

Overview

• Product nameAnti-MiTF antibodySee all MiTF primary antibodies ...
• Description
  Rabbit polyclonal to MiTF
• Tested applicationsIHC-P more details
• Species reactivity
  Reacts with: Human
  
• Immunogen

  Synthetic peptide (Human)

• Positive controlHumam Melanoma tissue.

Properties

• FormLiquid
• Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
• Storage bufferPreservative: 0.1% Sodium Azide
  Constituents: 1% BSA, 10mM PBS, pH 7.4
• Concentration information loading...
• PurityProtein A purified
• Clonality Polyclonal
• IsotypeIgG
• Research Areas
  • Epigenetics and Nuclear Signaling
  • Transcription
  • Domain Families
  • HLH / Leucine Zipper
  • HLH / Leucine Zipper

  • Immunology
  • Innate Immunity
  • Mast Cells

Applications

Target

• FunctionTranscription factor for tyrosinase and tyrosinase-related protein 1. Binds to a symmetrical DNA sequence (E-boxes) (5'-CACGTG-3') found in the tyrosinase promoter. Plays a critical role in the differentiation of various cell types as neural crest-derived melanocytes, mast cells, osteoclasts and optic cup-derived retinal pigment epithelium.
• Tissue specificityIsoform M is exclusively expressed in melanocytes and melanoma cells. Isoform A and isoform H are widely expressed in many cell types including melanocytes and retinal pigment epithelium (RPE). Isoform C is expressed in many cell types including RPE but not in melanocyte-lineage cells.
• Involvement in diseaseDefects in MITF are the cause of Waardenburg syndrome type 2A (WS2A) [MIM:193510]. It is a dominant inherited disorder characterized by sensorineural hearing loss and patches of depigmentation. The features show variable expression and penetrance.
  Defects in MITF are a cause of Waardenburg syndrome type 2 with ocular albinism (WS2-OA) [MIM:103470]. It is an ocular albinism with sensorineural deafness.
  Defects in MITF are the cause of Tietz syndrome (TIETZS) [MIM:103500]. It is an autosomal dominant disorder characterized by generalized hypopigmentation and profound, congenital, bilateral deafness. Penetrance is complete.
• Sequence similaritiesBelongs to the MiT/TFE family.
  Contains 1 basic helix-loop-helix (bHLH) domain.
• Post-translational
  modificationsPhosphorylation at Ser-405 significantly enhances the ability to bind the tyrosinase promoter.
• Cellular localizationNucleus.

Target information above from: UniProt accession O75030 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 4286 Human
  • Omim: 156845 Human
  • SwissProt: O75030 Human
  • Unigene: 166017 Human
  • Unigene: 618266 Human

• Alternative names
  BHLHE32 antibodybHLHe32 antibodyClass E basic helix-loop-helix protein 32 antibody

  CMM8 antibodyHomolog of mouse microphthalmia antibodyMi antibodyMicrophthalmia associated transcription factor antibodyMicrophthalmia, mouse, homolog of antibodyMicrophthalmia-associated transcription factor antibodyMITF antibodyMITF_HUMAN antibodyWS2 antibodyWS2A antibody

  see all

Anti-MiTF antibody images

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - MiTF antibody (ab73930)
  ab73930, at 1/100 dilution, staining MiTF in Human melanoma by Immunohistochemistry using formalin-fixed, paraffin-embedded tissue.