Anti-Microsomal Glutathione S-transferase 1 antibody [EPR7935] (ab129175)

Overview

• Product nameAnti-Microsomal Glutathione S-transferase 1 antibody [EPR7935]See all Microsomal Glutathione S-transferase 1 primary antibodies ...
• Description
  Rabbit monoclonal [EPR7935] to Microsomal Glutathione S-transferase 1
• Tested applicationsWB, ICC/IF more details
• Species reactivity
  Reacts with: Mouse, Rat, Human
  
• Immunogen

  Synthetic peptide corresponding to a region within the C-terminal of Human Microsomal Glutathione S-transferase 1.

• Positive controlFetal liver lysate, HepG2 cells and cell lysate, K562 cell lysate, HT 1376 cell lysate.
• General notesThis product was manufactured under U.S. Patent No. 5,675,063.

Properties

• FormLiquid
• Storage instructionsStore at -20°C. Stable for 12 months at -20°C
• Dissociation constant (Kd) Kd = 5.40 x 10^-12 M
• Storage bufferpH: 7.20
  Preservative: 0.01% Sodium azide
  Constituents: 49% PBS, 50% Glycerol, 0.05% BSA
• PurityTissue culture supernatant
• Clonality Monoclonal
• Clone numberEPR7935
• IsotypeIgG
• Research Areas
  • Metabolism
  • Pathways and Processes
  • Redox metabolism
  • Oxidative stress

  • Developmental Biology
  • Post embryonic development
  • Aging

  • Cell Biology
  • Other Antibodies
  • Oxidative Stress

  • Cell Biology
  • Other Antibodies
  • Drugs and Chemicals

Applications

Target

• FunctionConjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. Has a wide substrate specificity.
• Tissue specificityHighly expressed in liver.
• Sequence similaritiesBelongs to the MAPEG family.
• Post-translational
  modificationsPeroxynitrite induces nitration at Tyr-93 which activates the enzyme.
• Cellular localizationMicrosome. Mitochondrion outer membrane. Endoplasmic reticulum membrane.

Target information above from: UniProt accession P10620 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 4257 Human
  • Entrez Gene: 56615 Mouse
  • Entrez Gene: 171341 Rat
  • Omim: 138330 Human
  • SwissProt: P10620 Human
  • SwissProt: Q91VS7 Mouse
  • SwissProt: P08011 Rat
  • Unigene: 389700 Human

  • Unigene: 14796 Mouse
  • Unigene: 2580 Rat

  see all

• Alternative names
  MGST1 antibodyGlutathione S transferase 12 antibodyGST12 antibody

  MGST 1 antibodyMGST antibodyMGST1 antibodyMGST1_HUMAN antibodyMicrosomal glutathione S-transferase 1 antibodyMicrosomal GST 1 antibodyMicrosomal GST-1 antibodyMicrosomal GST-I antibody

  see all

Anti-Microsomal Glutathione S-transferase 1 antibody [EPR7935] images

• 

  Western blot - Anti-Microsomal Glutathione S-transferase 1 antibody [EPR7935] (ab129175)
  All lanes : Anti-Microsomal Glutathione S-transferase 1 antibody [EPR7935] (ab129175) at 1/1000 dilution
  
  Lane 1 : Fetal liver lysate
  Lane 2 : HepG2 cell lysate
  Lane 3 : K562 cell lysate
  Lane 4 : HT 1376 cell lysate
  
  Lysates/proteins at 10 µg per lane.
  
  Secondary
  Goat anti-rabbit HRP conjugated antibody. at 1/2000 dilution
  
  Predicted band size : 18 kDa
  Observed band size : 16 kDa (why is the actual band size different from the predicted?)
  
• 

  Immunocytochemistry/ Immunofluorescence - Anti-Microsomal Glutathione S-transferase 1 antibody [EPR7935] (ab129175)
  ab129175, at 1/100, staining Microsomal Glutathione S-transferase 1 in HepG2 cells by Immunofluorescence.
• 

  Other-Anti-Microsomal Glutathione S-transferase 1 antibody [EPR7935](ab129175)
  Equilibrium disassociation constant (K_D)
  Learn more about K_D
  
  Click here to learn more about K_D