![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Mineralocorticoid Receptor antibody [H3122] (ab41912) image](#ab41912_a.jpg)
 image](#Mineralocorticoid-Receptor-Primary-antibodies-ab41912-1.jpg)
![Flow Cytometry - Mineralocorticoid Receptor antibody [H3122] (ab41912) image](#Mineralocorticoid-Receptor-Primary-antibodies-ab41912-4.jpg)
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Products:Epigenetics and Nuclear Signaling >> Transcription >> Domain Families >> Zinc Finger
Overview
Product name
Anti-Mineralocorticoid Receptor antibody [H3122]
See all Mineralocorticoid Receptor products (5) ...
Description
Mouse monoclonal [H3122] to Mineralocorticoid Receptor
Specificity
In immunohistochemistry this antibody gives good staining in colon and kidney tissue. However, it has not been possible to detect endogenous expression by Western; as positive control a mouse cell line transfected with cDNA was used for this application.
Tested applications
ELISA, IP, IHC-P, WB, Flow Cyt, ICC/IFmore details
Cross reactivity
Reacts with
Mouse, Rat, Human
Predicted to work with
Monkey, Marmoset (common)
Immunogen
Baculovirus-expressed recombinant fragment: ETKGYHSLPE GLDMERRWGQ VSQAVERSSL GPTERTDENN YMEIVNVSCV SGAIPNNSTQ GSSKEKQELL PCLQQDNNRP GILTSDIKTE LESKELSA, corresponding to amino acids 2-99 of Human Mineralocorticoid Receptor.
ETKGYHSLPE GLDMERRWGQ VSQAVERSSL GPTERTDENN YMEIVNVSCV SGAIPNNSTQ GSSKEKQELL PCLQQDNNRP GILTSDIKTE LESKELSA
Positive control
IHC: Human and rat large intestine epithelial cells. WB: COS-7 cell lysate transfected with cDNA
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.
Storage buffer
Physiological Saline. Preservative: 0.1% Sodium Azide
Concentration
Concentration information loading...
Purification notes
Purified from ascites after inoculation with hybridoma of mouse myeloma cells via ammonium sulfate fractionation.
Clonality
Monoclonal
Clone number
H3122
Isotype
IgG2a
Research areas
Cardiovascular >> Heart >> Hypertrophy >> Other
Cardiovascular >> Atherosclerosis >> Hypertension >> Vascular remodelling >> Hypertrophy
Epigenetics and Nuclear Signaling >> Nuclear Signaling Pathways >> Nuclear Receptors >> Corticoid
Signal Transduction >> Signaling Pathway >> Nuclear Signaling >> Nuclear Hormone Receptors >> Corticoid
Epigenetics and Nuclear Signaling >> Transcription >> Domain Families >> Zinc Finger
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Mineralocorticoid Receptor antibody [H3122] (ab41912)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Mineralocorticoid Receptor antibody [H3122] (ab41912) image](/ps/datasheet/Images/41/ab41912/ab41912_a.jpg)
(enlarge)
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Immunocytochemistry/ Immunofluorescence-Mineralocorticoid Receptor antibody [H3122](ab41912)
 image](/ps/datasheet/images/41/ab41912/Mineralocorticoid-Receptor-Primary-antibodies-ab41912-1.jpg)
(enlarge)
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Flow Cytometry - Mineralocorticoid Receptor antibody [H3122] (ab41912)
![Flow Cytometry - Mineralocorticoid Receptor antibody [H3122] (ab41912) image](/ps/datasheet/images/41/ab41912/Mineralocorticoid-Receptor-Primary-antibodies-ab41912-4.jpg)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab41912 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ELISA: Use a concentration of 0.2 µg/ml
IP: Use at an assay dependent dilution.
IHC-P: 1/20 - 1/40.
WB: Use a concentration of 1 µg/mlPredicted molecular weight: 107 kDa.(Block with 5% milk and incubate primary antibody in blocking buffer.)
Flow Cyt: Use at an assay dependent dilution.
ICC/IF: Use a concentration of 1 µg/ml
Target
Function
Receptor for both mineralocorticoids (MC) such as aldosterone and glucocorticoids (GC) such as corticosterone or cortisol. Binds to mineralocorticoid response elements (MRE) and transactivates target genes. The effect of MC is to increase ion and water transport and thus raise extracellular fluid volume and blood pressure and lower potassium levels.
Tissue specificity
Ubiquitous. Highly expressed in distal tubules, convoluted tubules and cortical collecting duct in kidney, and in sweat glands. Detected at lower levels in cardiomyocytes, in epidermis and in colon enterocytes.
Involvement in disease
Defects in NR3C2 are a cause of autosomal dominant pseudohypoaldosteronism type I (AD-PHA1) [MIM:177735]. PHA1 is characterized by urinary salt wasting, resulting from target organ unresponsiveness to mineralocorticoids. There are 2 forms of PHA1: the autosomal dominant form that is mild, and the recessive form which is more severe and due to defects in any of the epithelial sodium channel subunits. In AD-PHA1 the target organ defect is confined to kidney. Clinical expression can vary from asymptomatic to moderate. It may be severe at birth, but symptoms remit with age. Familial and sporadic cases have been reported.
Defects in NR3C2 are a cause of early-onset hypertension with severe exacerbation in pregnancy (EOHSEP) [MIM:605115]. Inheritance is autosomal dominant. The disease is characterized by the onset of severe hypertension before the age of 20, and by suppression of aldosterone secretion.
Sequence similarities
Belongs to the nuclear hormone receptor family. NR3 subfamily.
Contains 1 nuclear receptor DNA-binding domain.
Domain
Composed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal ligand-binding domain.
Post-translational
modifications
Phosphorylated.
Cellular localization
Cytoplasm. Nucleus. Endoplasmic reticulum membrane. Cytoplasmic and nuclear in the absence of ligand; nuclear after ligand-binding. When bound to HSD11B2, it is found associated with the endoplasmic reticulum membrane.
Target information above from: UniProt accessionP08235
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Aldosterone receptor antibody
- MCR antibody
- MCR_HUMAN antibody
see all
Database links
- Entrez Gene: 4306 Human
- Entrez Gene: 110784 Mouse
- Entrez Gene: 25672 Rat
- Omim: 600983 Human
- SwissProt: P08235 Human
- SwissProt: Q8VII8 Mouse
- SwissProt: P22199 Rat
- Unigene: 163924 Human
see all
Anti-Mineralocorticoid Receptor antibody [H3122] images:
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Mineralocorticoid Receptor antibody [H3122] (ab41912)
ab41912 staining Mineralocorticoid Receptor in human large intestine paraffin section (20-40ug/mL) by Immunohistochemistry, formalin-fixed paraffin embedded sections.
Immunocytochemistry/ Immunofluorescence-Mineralocorticoid Receptor antibody [H3122](ab41912)
ICC/IF image of ab41912 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab41912, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Flow Cytometry - Mineralocorticoid Receptor antibody [H3122] (ab41912)
ab41912 used in Flow Cytometry.Human peripheral blood leukocyte cells were fixed with paraformaldehyde, permeabilized with saponin and then stained with ab41912 (which had been directly conjugated to Alexa Fluor 647), used at a 1/50 dilution. Gated on CD3+ lymphocytes (T cells).
Image courtesy of Luciana Besedovsky by Abreview.
Protocols
References for Anti-Mineralocorticoid Receptor antibody [H3122] (ab41912)
This product has been referenced in:
- Fraccarollo Det al. Deletion of cardiomyocyte mineralocorticoid receptor ameliorates adverse remodeling after myocardial infarction. Circulation 123:400-8 (2011). WB; Mouse.Read more (PubMed: 21242479) »
- López-Andrés Net al. Aldosterone induces cardiotrophin-1 expression in HL-1 adult cardiomyocytes. Endocrinology 149:4970-8 (2008).Read more (PubMed: 18566129) »
See all 2 publications for this product
Publishing research using ab41912? Please let us know so that we can cite the reference in this datasheet
![Mineralocorticoid Receptor antibody [H3122] for Flow Cytometry in Human (41912)](/ps/datasheet/images/41/ab41912/Mineralocorticoid-Receptor-Primary-antibodies-ab41912-3.jpg)
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"