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Products:Epigenetics and Nuclear Signaling >> Transcription >> Domain Families >> Zinc Finger
Overview
Product name
Anti-Mineralocorticoid Receptor antibody
See all Mineralocorticoid Receptor products (5) ...
Description
Rabbit polyclonal to Mineralocorticoid Receptor
Tested applications
WB, IHC-Pmore details
Cross reactivity
Reacts with
Human
Predicted to work with
Mouse, Rat, Chicken
Immunogen
A region within synthetic peptide: KIRRKNCPAC RLQKCLQAGM NLGARKSKKL GKLKGIHEEQ PQQQQPPPPP, corresponding to internal sequence amino acids 649-698 of Human Mineralocorticoid Receptor
KIRRKNCPAC RLQKCLQAGM NLGARKSKKL GKLKGIHEEQ PQQQQPPPPP
Positive control
A172 cell lysate. Human kidney tissue.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: 2% Sucrose, PBS
Concentration
Concentration information loading...
Purity
Protein A purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Cardiovascular >> Heart >> Hypertrophy >> Other
Cardiovascular >> Atherosclerosis >> Hypertension >> Vascular remodelling >> Hypertrophy
Epigenetics and Nuclear Signaling >> Nuclear Signaling Pathways >> Nuclear Receptors >> Corticoid
Signal Transduction >> Signaling Pathway >> Nuclear Signaling >> Nuclear Hormone Receptors >> Corticoid
Epigenetics and Nuclear Signaling >> Transcription >> Domain Families >> Zinc Finger
Applications
Show applications key
Our Abpromise guarantee covers the use of ab62532 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use a concentration of 1.25 µg/mlDetects a band of approximately 90 kDa (predicted molecular weight: 107 kDa).Can be blocked with Mineralocorticoid Receptor peptide (649-698) (ab87358).
IHC-P: Use a concentration of 4.0 - 8.0 µg/ml.
Target
Function
Receptor for both mineralocorticoids (MC) such as aldosterone and glucocorticoids (GC) such as corticosterone or cortisol. Binds to mineralocorticoid response elements (MRE) and transactivates target genes. The effect of MC is to increase ion and water transport and thus raise extracellular fluid volume and blood pressure and lower potassium levels.
Tissue specificity
Ubiquitous. Highly expressed in distal tubules, convoluted tubules and cortical collecting duct in kidney, and in sweat glands. Detected at lower levels in cardiomyocytes, in epidermis and in colon enterocytes.
Involvement in disease
Defects in NR3C2 are a cause of autosomal dominant pseudohypoaldosteronism type I (AD-PHA1) [MIM:177735]. PHA1 is characterized by urinary salt wasting, resulting from target organ unresponsiveness to mineralocorticoids. There are 2 forms of PHA1: the autosomal dominant form that is mild, and the recessive form which is more severe and due to defects in any of the epithelial sodium channel subunits. In AD-PHA1 the target organ defect is confined to kidney. Clinical expression can vary from asymptomatic to moderate. It may be severe at birth, but symptoms remit with age. Familial and sporadic cases have been reported.
Defects in NR3C2 are a cause of early-onset hypertension with severe exacerbation in pregnancy (EOHSEP) [MIM:605115]. Inheritance is autosomal dominant. The disease is characterized by the onset of severe hypertension before the age of 20, and by suppression of aldosterone secretion.
Sequence similarities
Belongs to the nuclear hormone receptor family. NR3 subfamily.
Contains 1 nuclear receptor DNA-binding domain.
Domain
Composed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal ligand-binding domain.
Post-translational
modifications
Phosphorylated.
Cellular localization
Cytoplasm. Nucleus. Endoplasmic reticulum membrane. Cytoplasmic and nuclear in the absence of ligand; nuclear after ligand-binding. When bound to HSD11B2, it is found associated with the endoplasmic reticulum membrane.
Target information above from: UniProt accessionP08235
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Aldosterone receptor antibody
- MCR antibody
- MCR_HUMAN antibody
see all
Database links
- Entrez Gene: 374131 Chicken
- Entrez Gene: 4306 Human
- Entrez Gene: 110784 Mouse
- Entrez Gene: 25672 Rat
- Omim: 600983 Human
- SwissProt: P08235 Human
- SwissProt: Q8VII8 Mouse
- SwissProt: P22199 Rat
see all
Anti-Mineralocorticoid Receptor antibody images:
Western blot - Mineralocorticoid Receptor antibody (ab62532)
Anti-Mineralocorticoid Receptor antibody (ab62532) at 1.25 µg/ml + A172 cell lysate at 10 µg
Secondary
HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size : 107 kDa
Observed band size : 90 kDa (why is the actual band size different from the predicted?)
Gel concentration: 8%
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Mineralocorticoid Receptor antibody (ab62532)
ab62532 (4-8ug/ml) staining Mineralocorticoid Receptor in human kidney tissue by immunohistochemistry using paraffin-embedded tissue. The arrows indicate positively stained epithelial cells of the renal tubule.
References for Anti-Mineralocorticoid Receptor antibody (ab62532)
ab62532 has not yet been referenced specifically in any publications.
Publishing research using ab62532? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"