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Western blot - Mineralocorticoid Receptor antibody (ab64457)
Western blot - Mineralocorticoid Receptor antibody (ab64457)
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Immunocytochemistry - Mineralocorticoid Receptor antibody (ab64457)
Immunocytochemistry - Mineralocorticoid Receptor antibody (ab64457)
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Mineralocorticoid Receptor antibody (ab64457)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Mineralocorticoid Receptor antibody (ab64457)
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Product Name 

Mineralocorticoid Receptor antibody

 

See all Mineralocorticoid Receptor antibodies (4)...

Product type 

Primary antibodies

Description 

Rabbit polyclonal to Mineralocorticoid Receptor

Immunogen 

Synthetic peptide conjugated to KLH derived from within residues 950 to the C-terminus of Human Mineralocorticoid Receptor.

(Peptide available as ab74464.)

Reacts with 

Hu, Ms

Predicted to react 
(species key)

Rat, NHuPrm, Xl
(Due to sequence homology)

Tested applications 
(see key)

ICC/IF, IHC-P, WB


Abreviews 

Customer reviews
(see key)


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Application notes 
(see key)

Recommended dilutions
ICC/IF: Use at a concentration of 1 µg/ml.
IHC-P: Use at a concentration of 1 µg/ml.
WB: Use at a concentration of 1 µg/ml. Detects a band of approximately 100 kDa (predicted molecular weight: 107 kDa).


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

This antibody gave a positive signal in the following lysates:
Mouse Kidney Tissue
Human Small Intestine Tissue
Human Colon Tissue

Cellular localization 

Cytoplasm. Nucleus. Endoplasmic reticulum membrane; Peripheral membrane protein. Note=Cytoplasmic and nuclear in the absence of ligand; nuclear after ligand-binding. When bound to HSD11B2, it is found associated with the endoplasmic reticulum membrane.

Research areas 

Cardiovascular >> Heart >> Hypertrophy >> Other
Cardiovascular >> Atherosclerosis >> Hypertension >> Vascular remodelling >> Hypertrophy
Chromatin and Nuclear Signaling >> Nuclear Signaling Pathways >> Nuclear Receptors >> Corticoid
Signal Transduction >> Signaling Pathway >> Nuclear Signaling >> Nuclear Hormone Receptors >> Corticoid
Chromatin and Nuclear Signaling >> Transcription >> Domain Families >> Zinc Finger

Relevance 

Steroid receptors are ligand dependent, intracellular proteins that stimulate transcription of specific genes by binding to specific DNA sequences following activation by the appropriate hormone. Mineralocorticoids are a family of steroids, secreted by the adrenal cortex, necessary for the regulation of a number of metabolic processes including electrolyte regulation. These compounds exert their effect through their interaction with the mineralocorticoid receptor and that complex's subsequent association with DNA. Given the function of mineralocorticoids, it is not surprising to find that the kidney is a primary target organ for mineralocorticoids and that this organ has been shown to contain Mineralocorticoid Receptor.

 

Mineralocorticoid Receptor antibody (ab64457)

Database links 

The links below go to external sites and will open in a new browser window

Entrez Gene

    

4306    (Human)
110784    (Mouse)
25672    (Rat)
399290    (Xenopus laevis)

GeneCard

    

GC04M149357    (Human)

Omim

    

600983    (Human)

SwissProt

    

P08235    (Human)
Q8VII8    (Mouse)
P22199    (Rat)
Q66J29    (Xenopus laevis)

Unigene

    

163924    (Human)

Raised in 

Rabbit

Clonality 

Polyclonal

Isotype 

IgG

Purity 

Immunogen affinity purified

Storage buffer 

Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4

Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS

Form 

Liquid

Concentration 

1.000 mg/ml

Storage instructions 

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

 

At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this Mineralocorticoid Receptor antibody is on this datasheet. But please do contact us if you would like any reassurance!


See below for Mineralocorticoid Receptor antibody images, references, products related to ab64457 and other tools.

 

Mineralocorticoid Receptor antibody images:

Product image  Western blot - Mineralocorticoid Receptor antibody (ab64457)

Western blot - Mineralocorticoid Receptor antibody (ab64457)

All lanes : Mineralocorticoid Receptor antibody (ab64457) at 1 µg/ml

Lane 1 : Kidney (Mouse) Tissue Lysate
Lane 2 : Small Intestine (Human) Tissue Lysate - adult normal tissue (ab29276)
Lane 3 : Colon tissue lysate (Human) Tissue Lysate - adult normal tissue (ab30051)

Lysates/proteins at 10 µg per lane.

Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

Performed under reducing conditions.

Predicted band size : 107 kDa
Observed band size : 100 kDa (why is the actual band size different from the predicted?)
Additional bands at : 26 kDa. We are unsure as to the identity of these extra bands.

Exposure time : 30 seconds

The 100 kDa band observed is comparable to the molecular weight seen with other commercially available antibodies to mineralocorticoid receptor.

 

Product image  Immunocytochemistry - Mineralocorticoid Receptor antibody (ab64457)

Immunocytochemistry - Mineralocorticoid Receptor antibody (ab64457)

ICC/IF image of ab64457 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab64457, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 4% PFA fixed (10 min) HepG2, Hek293 and MCF7 cells at 1µg/ml, and in 100% methanol fixed (5 min) HeLa, Hek293, HepG2 and MCF7 cells at 1µg/ml.

 

Product image  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Mineralocorticoid Receptor antibody (ab64457)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Mineralocorticoid Receptor antibody (ab64457)

IHC image of Mineralocorticoid Receptor staining in Human Tonsil FFPE section, performed on a BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab64457, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX

 
 

Search PubMed (MEDLINE) for references to Mineralocorticoid Receptor

 

Mineralocorticoid Receptor antibody - more information

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Protocols for Mineralocorticoid Receptor antibody

Price and availability of products related to Mineralocorticoid Receptor antibody

 

Mineralocorticoid Receptor antibody - related products:

Products for Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Slides

Products for Western blot

Loading controls

Lysates

 

Immunizing Peptide for Mineralocorticoid Receptor antibody

ab74464

  

Mineralocorticoid Receptor peptide

 

Compatible Secondaries for Mineralocorticoid Receptor antibody

ab6564

  

Cy5 ® antibody

ab6717

  

FITC antibody

ab6720

  

Biotin antibody

ab6721

  

HRP antibody

ab6722

  

Alkaline Phosphatase antibody

ab6793

  

Texas Red ® antibody

ab7007

  

Phycoerythrin antibody

ab7019

  

Agarose antibody

ab27234

  

10nm Gold antibody

ab27235

  

5nm Gold antibody

ab27236

  

15nm Gold antibody

ab27237

  

20nm Gold antibody

ab27238

  

30nm Gold antibody

ab27239

  

10nm Gold antibody

ab27240

  

5nm Gold antibody

ab31193

  

Duck polyclonal to Rabbit IgG

ab60314

  

Chromeo™ 488 antibody

ab60315

  

Chromeo™ 494 antibody

ab60317

  

Chromeo™ 546 antibody

ab60319

  

Chromeo™ 642 antibody

 

Other Mineralocorticoid Receptor Antibodies

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Mineralocorticoid Receptor antibody - other tools:

Contact Abcam with a Technical Enquiry about Mineralocorticoid Receptor antibody

 

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