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Products:Epigenetics and Nuclear Signaling >> Transcription >> Domain Families >> Zinc Finger
Overview
Product name
Anti-Mineralocorticoid Receptor antibody
See all Mineralocorticoid Receptor products (5) ...
Description
Rabbit polyclonal to Mineralocorticoid Receptor
Tested applications
WB, IHC-P, ICC/IFmore details
Cross reactivity
Reacts with
Mouse, Human
Predicted to work with
Rat, Xenopus laevis, Non Human Primates
Immunogen
Synthetic peptide conjugated to KLH derived from within residues 950 to the C-terminus of Human Mineralocorticoid Receptor.
(Peptide available as ab74464.)
Positive control
This antibody gave a positive signal in the following lysates: Mouse Kidney Tissue Human Small Intestine Tissue Human Colon Tissue
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Cardiovascular >> Heart >> Hypertrophy >> Other
Cardiovascular >> Atherosclerosis >> Hypertension >> Vascular remodelling >> Hypertrophy
Epigenetics and Nuclear Signaling >> Nuclear Signaling Pathways >> Nuclear Receptors >> Corticoid
Signal Transduction >> Signaling Pathway >> Nuclear Signaling >> Nuclear Hormone Receptors >> Corticoid
Epigenetics and Nuclear Signaling >> Transcription >> Domain Families >> Zinc Finger
Applications
Show applications key
Our Abpromise guarantee covers the use of ab64457 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use a concentration of 1 µg/mlDetects a band of approximately 100 kDa (predicted molecular weight: 107 kDa).
IHC-P: Use a concentration of 1 µg/ml
ICC/IF: Use a concentration of 1 µg/ml
Target
Function
Receptor for both mineralocorticoids (MC) such as aldosterone and glucocorticoids (GC) such as corticosterone or cortisol. Binds to mineralocorticoid response elements (MRE) and transactivates target genes. The effect of MC is to increase ion and water transport and thus raise extracellular fluid volume and blood pressure and lower potassium levels.
Tissue specificity
Ubiquitous. Highly expressed in distal tubules, convoluted tubules and cortical collecting duct in kidney, and in sweat glands. Detected at lower levels in cardiomyocytes, in epidermis and in colon enterocytes.
Involvement in disease
Defects in NR3C2 are a cause of autosomal dominant pseudohypoaldosteronism type I (AD-PHA1) [MIM:177735]. PHA1 is characterized by urinary salt wasting, resulting from target organ unresponsiveness to mineralocorticoids. There are 2 forms of PHA1: the autosomal dominant form that is mild, and the recessive form which is more severe and due to defects in any of the epithelial sodium channel subunits. In AD-PHA1 the target organ defect is confined to kidney. Clinical expression can vary from asymptomatic to moderate. It may be severe at birth, but symptoms remit with age. Familial and sporadic cases have been reported.
Defects in NR3C2 are a cause of early-onset hypertension with severe exacerbation in pregnancy (EOHSEP) [MIM:605115]. Inheritance is autosomal dominant. The disease is characterized by the onset of severe hypertension before the age of 20, and by suppression of aldosterone secretion.
Sequence similarities
Belongs to the nuclear hormone receptor family. NR3 subfamily.
Contains 1 nuclear receptor DNA-binding domain.
Domain
Composed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal ligand-binding domain.
Post-translational
modifications
Phosphorylated.
Cellular localization
Cytoplasm. Nucleus. Endoplasmic reticulum membrane. Cytoplasmic and nuclear in the absence of ligand; nuclear after ligand-binding. When bound to HSD11B2, it is found associated with the endoplasmic reticulum membrane.
Target information above from: UniProt accessionP08235
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Aldosterone receptor antibody
- MCR antibody
- MCR_HUMAN antibody
see all
Database links
- Entrez Gene: 4306 Human
- Entrez Gene: 110784 Mouse
- Entrez Gene: 25672 Rat
- Entrez Gene: 399290 Xenopus laevis
- Omim: 600983 Human
- SwissProt: P08235 Human
- SwissProt: Q8VII8 Mouse
- SwissProt: P22199 Rat
see all
Anti-Mineralocorticoid Receptor antibody images:
Western blot - Mineralocorticoid Receptor antibody (ab64457)
All lanes : Anti-Mineralocorticoid Receptor antibody (ab64457) at 1 µg/ml
Lane 1 : Kidney (Mouse) Tissue Lysate
Lane 2 : Small Intestine (Human) Tissue Lysate - adult normal tissue (ab29276)
Lane 3 : Colon tissue lysate (Human) Tissue Lysate - adult normal tissue (ab30051)
Lysates/proteins at 10 µg per lane.
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size : 107 kDa
Observed band size : 100 kDa (why is the actual band size different from the predicted?)
Additional bands at : 26 kDa. We are unsure as to the identity of these extra bands.
Exposure time : 30 seconds
The 100 kDa band observed is comparable to the molecular weight seen with other commercially available antibodies to mineralocorticoid receptor.
Immunocytochemistry - Mineralocorticoid Receptor antibody (ab64457)
ICC/IF image of ab64457 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab64457, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 4% PFA fixed (10 min) HepG2, Hek293 and MCF7 cells at 1µg/ml, and in 100% methanol fixed (5 min) HeLa, Hek293, HepG2 and MCF7 cells at 1µg/ml.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Mineralocorticoid Receptor antibody (ab64457)
IHC image of Mineralocorticoid Receptor staining in Human Tonsil FFPE section, performed on a BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab64457, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX
References for Anti-Mineralocorticoid Receptor antibody (ab64457)
This product has been referenced in:
- Lin CHet al. Reverse Effect of Mammalian Hypocalcemic Cortisol in Fish: Cortisol Stimulates Ca Uptake via Glucocorticoid Receptor-Mediated Vitamin D(3) Metabolism. PLoS One 6:e23689 (2011). WB; Zebrafish.Read more (PubMed: 21887296) »
See 1 publication for this product
Publishing research using ab64457? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"