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Western blot - Mineralocorticoid Receptor antibody (ab64457)
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Immunocytochemistry - Mineralocorticoid Receptor antibody (ab64457)
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Mineralocorticoid Receptor antibody (ab64457)
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Product Name
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Mineralocorticoid Receptor antibody
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See all Mineralocorticoid Receptor antibodies (4)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to Mineralocorticoid Receptor
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Immunogen
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Synthetic peptide conjugated to KLH derived from within residues 950 to the C-terminus of Human Mineralocorticoid Receptor. (Peptide available as ab74464.)
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Reacts with
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Hu, Ms
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Predicted to react
(species key)
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Rat, NHuPrm, Xl
(Due to sequence homology)
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Tested applications
(see key)
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ICC/IF, IHC-P, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ICC/IF: Use at a concentration of 1 µg/ml.
IHC-P: Use at a concentration of 1 µg/ml.
WB: Use at a concentration of 1 µg/ml. Detects a band of approximately 100 kDa (predicted molecular weight: 107 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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This antibody gave a positive signal in the following lysates:
Mouse Kidney Tissue
Human Small Intestine Tissue
Human Colon Tissue
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Cellular localization
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Cytoplasm. Nucleus. Endoplasmic reticulum membrane; Peripheral membrane protein. Note=Cytoplasmic and nuclear in the absence of ligand; nuclear after ligand-binding. When bound to HSD11B2, it is found associated with the endoplasmic reticulum membrane.
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Research areas
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Cardiovascular >> Heart >> Hypertrophy >> Other
Cardiovascular >> Atherosclerosis >> Hypertension >> Vascular remodelling >> Hypertrophy
Chromatin and Nuclear Signaling >> Nuclear Signaling Pathways >> Nuclear Receptors >> Corticoid
Signal Transduction >> Signaling Pathway >> Nuclear Signaling >> Nuclear Hormone Receptors >> Corticoid
Chromatin and Nuclear Signaling >> Transcription >> Domain Families >> Zinc Finger
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Relevance
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Steroid receptors are ligand dependent, intracellular proteins that stimulate transcription of specific genes by binding to specific DNA sequences following activation by the appropriate hormone. Mineralocorticoids are a family of steroids, secreted by the adrenal cortex, necessary for the regulation of a number of metabolic processes including electrolyte regulation. These compounds exert their effect through their interaction with the mineralocorticoid receptor and that complex's subsequent association with DNA. Given the function of mineralocorticoids, it is not surprising to find that the kidney is a primary target organ for mineralocorticoids and that this organ has been shown to contain Mineralocorticoid Receptor.
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Mineralocorticoid Receptor antibody (ab64457) |
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4 Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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1.000 mg/ml
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Storage instructions
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Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this Mineralocorticoid Receptor antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for Mineralocorticoid Receptor antibody images, references, products related to ab64457 and other tools.
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Mineralocorticoid Receptor antibody images:
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 Immunocytochemistry - Mineralocorticoid Receptor antibody (ab64457)
ICC/IF image of ab64457 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab64457, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 4% PFA fixed (10 min) HepG2, Hek293 and MCF7 cells at 1µg/ml, and in 100% methanol fixed (5 min) HeLa, Hek293, HepG2 and MCF7 cells at 1µg/ml.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Mineralocorticoid Receptor antibody (ab64457)
IHC image of Mineralocorticoid Receptor staining in Human Tonsil FFPE section, performed on a BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab64457, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX
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Search PubMed (MEDLINE) for references to Mineralocorticoid Receptor
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Mineralocorticoid Receptor antibody - more information
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Customer reviews (feedback) regarding Mineralocorticoid Receptor antibody |
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Mineralocorticoid Receptor antibody - related products:
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