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Products:Neuroscience >> Neurology process >> Neurodegenerative disease >> Alzheimer's disease >> Amyloid
Overview
Product name
Anti-APBA1 antibody
See all APBA1 products (6) ...
Description
Rabbit polyclonal to APBA1
Specificity
Detects APBA1 (Mint 1) from rat brain extract as well as extract from HEK293 cells overexpressing the rat gene.
Tested applications
Cross reactivity
Reacts with
Rat
Immunogen
Synthetic peptide: MNHLEGSAEVEVADEAP-C, corresponding to amino acids 1-17 of Rat APBA1 (Mint1).
MNHLEGSAEV EVADEAP-C
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
PBS, 1 mg/ml BSA and 0.05% sodium azide
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Primary antibody notes
The munc-18 interacting (Mint) protein family is a group of evolutionarily conserved adaptor proteins that function in membrane transport and organization. In mammals, there exist three Mint isoforms, Mint 1, 2, and 3. Although there is little amino acid sequence conservation in the amino-terminal half, the carboxy-terminal half of these proteins is highly conserved. Within this conserved portion there exists a phosphotyrosine-binding (PTB) and a PSD-95/DLG-A/ZO-1 (PDZ) domain, which function as protein interaction modules. Mint 1 and 2 appear to be expressed exclusively in the brain and are found to bind to Munc18, an essential component of the synaptic vesicle fusion machinery. Mint 3 is ubiquitously expressed in all tissues and is expressed at the lowest levels in the brain and testis. Studies show that Mint3 does not interact with munc-18. Mint 3 has been found to interact with the Alzheimer’s Disease-related amyloid precursor protein (APP) and does so through its PTB and PDZ domains. It has been suggested that Mint3 links APP to other transport machinery components, thereby regulating it transport, endocytosis, and metabolism. Abnormal APP metabolism has been shown to be the cause of an early-onset type of Alzheimer’s disease.
Clonality
Polyclonal
Isotype
IgG
Research areas
Neuroscience >> Cell Type Marker >> Neuron marker >> Synapse marker
Neuroscience >> Neurotransmission >> Secretory Vesicles >> Other
Neuroscience >> Neurology process >> Neurodegenerative disease >> Alzheimer's disease >> Other
Neuroscience >> Neurology process >> Neurodegenerative disease >> Alzheimer's disease >> Amyloid
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Western blot - APBA1 antibody (ab3448)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab3448 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
- 1 Image
WB
Application notes
Western Blot: use at a concentration of 1 µg/ml.
Not tested in other applications.
Optimal dilutions / concentrations should be determined by the end user.
By Western blot, this antibody detects an ~130 kDa protein representing Mint 1 from rat forebrain postnuclear supernatant.
Target
Function
Putative function in synaptic vesicle exocytosis by binding to Munc18-1, an essential component of the synaptic vesicle exocytotic machinery. May modulate processing of the beta-amyloid precursor protein (APP) and hence formation of beta-APP.
Tissue specificity
Brain and spinal cord.
Sequence similarities
Contains 2 PDZ (DHR) domains.
Contains 1 PID domain.
Domain
Composed of an N-terminal domain that binds Munc18-1 and LIN-2/CASK, a middle phosphotyrosine-binding domain (PID/PTB) that mediates binding with the cytoplasmic domain of the beta-amyloid precursor protein, and two C-terminal PDZ domains thought to attach proteins to the plasma membrane.
Target information above from: UniProt accessionQ02410
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Adapter protein X11 alpha antibody
- Adapter protein X11alpha antibody
- Amyloid beta A4 precursor protein-binding family A member 1 antibody
see all
Database links
Anti-APBA1 antibody images:
Western blot - APBA1 antibody (ab3448)
Western blot of APBA1 on extract from HEK293 cells overexpressing the rat gene using ab3448.
References for Anti-APBA1 antibody (ab3448)
This product has been referenced in:
- Tomas Aet al. Munc 18-1 and granuphilin collaborate during insulin granule exocytosis. Traffic 9:813-32 (2008).Read more (PubMed: 18208509) »
See 1 publication for this product
Publishing research using ab3448? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"