APBA1 antibody (ab3448)

Product Name 

APBA1 antibody

Product type 

Primary antibodies

Description 

Rabbit polyclonal to APBA1

Immunogen 

Synthetic peptide: MNHLEGSAEVEVADEAP-C, corresponding to amino acids 1-17 of Rat APBA1 (Mint1).
BLAST 'MNHLEGSAEVEVADEAP-C' with or

Reacts with 
(species key)

Rat

Specificity 

Detects APBA1 (Mint 1) from rat brain extract as well as extract from HEK293 cells overexpressing the rat gene.

Tested applications 
(see key)

WB

Abreviews 

Customer reviews (see key)

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Application notes 
(see key)

Recommended dilutions
Western Blot: use at a concentration of 1 µg/ml.
Not tested in other applications.

Optimal dilutions / concentrations should be determined by the end user.

By Western blot, this antibody detects an ~130 kDa protein representing Mint 1 from rat forebrain postnuclear supernatant.

Cellular localization 

Cytoplasmic

Research areas 

Neuroscience >> Cell Type Marker >> Neuron marker >> Synapse marker
Neuroscience >> Neurotransmission >> Secretory Vesicles >> Other
Neuroscience >> Neurology process >> Neurodegenerative disease >> Alzheimer's disease >> Other
Neuroscience >> Neurology process >> Neurodegenerative disease >> Alzheimer's disease >> Amyloid

Relevance 

APBA1 is part of two multimeric complexes, the first containing Munc18-1 and syntaxin 1 and the second being a brain specific heterotrimeric complex with LIN-10/X11-alpha, LIN-2/CASK, and LIN7. It binds to the cytoplasmic domain of amyloid protein (APP). ABPA1 has a putative function in synaptic vesicle exocytosis by binding to Munc18-1, an essential component of the synaptic vesicle exocytotic machinery. It may modulate processing of the beta amyloid precursor protein (APP) and hence formation of beta APP.

The munc-18 interacting (Mint) protein family is a group of evolutionarily conserved adaptor proteins that function in membrane transport and organization. In mammals, there exist three Mint isoforms, Mint 1, 2, and 3. Although there is little amino acid sequence conservation in the amino-terminal half, the carboxy-terminal half of these proteins is highly conserved. Within this conserved portion there exists a phosphotyrosine-binding (PTB) and a PSD-95/DLG-A/ZO-1 (PDZ) domain, which function as protein interaction modules. Mint 1 and 2 appear to be expressed exclusively in the brain and are found to bind to Munc18, an essential component of the synaptic vesicle fusion machinery. Mint 3 is ubiquitously expressed in all tissues and is expressed at the lowest levels in the brain and testis. Studies show that Mint3 does not interact with munc-18. Mint 3 has been found to interact with the Alzheimer’s Disease-related amyloid precursor protein (APP) and does so through its PTB and PDZ domains. It has been suggested that Mint3 links APP to other transport machinery components, thereby regulating it transport, endocytosis, and metabolism. Abnormal APP metabolism has been shown to be the cause of an early-onset type of Alzheimer’s disease.

Database links 

Raised in 

Rabbit

Clonality 

Polyclonal

Isotype 

IgG

Purity 

Immunogen affinity purified

Storage buffer 

PBS, 1 mg/ml BSA and 0.05% sodium azide

Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS

Form 

Liquid

Concentration 

1.000 mg/ml

Storage instructions 

Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

See below for APBA1 antibody images, references, products related to ab3448 and other tools.

APBA1 antibody images:

References for APBA1 antibody (ab3448)

  This product has been used in:

Tomas A et al. Munc 18-1 and granuphilin collaborate during insulin granule exocytosis. Traffic 9:813-32 (2008). PubMed: 18208509

If you publish research using ab3448 please let us know so that we can cite the reference on this datasheet.

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