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Read our guarantee »Overview
Product name
MitoProfile® Pyruvate dehydrogenase (PDH) WB Antibody Cocktail
Assay type
Quantitative
Cross reactivity
Reacts with
Cow, Human
Product overview
ab110416 (MSP02) contains a total of 150 µg of monoclonal antibodies reactive against PDH subunits. Isolated mitochondria are available from several species for use as control samples.
The PDH Western blotting kit contains four different mAbs reacting specifically with E1 alpha, E1 beta, E2 and E2/E3bp. A mAb against the OSCP subunit of ATP synthase is included as a control. This mAb mixture can be used to probe the isolated pyruvate dehydrogenase for integrity and purity.
Cocktail Antibodies:
Mouse anti C-V-OSCP [4C11C10D12] monoclonal:
Amount: 12.5 µg
Working concentration: 0.5 µg/ml
Mouse anti PDH-E1-alpha [9H9AF5] monoclonal:
Amount: 25 µg
Working concentration: 1 µg/ml
Mouse anti PDH-E1-beta [17A5E2H8] monoclonal:
Amount: 62.5 µg
Working concentration: 2.5 µg/ml
Mouse anti PDH-E2 [15D3G9C11] monoclonal:
Amount: 25 µg
Working concentration: 1 µg/ml
Mouse anti PDH-E2/E3bp [13G2AE2BH5] monoclonal:
Amount: 25 µg
Working concentration: 1 µg/ml
Tested applications
Properties
Storage instructions
Store at +4°C. Do not freeze.
| Components | 150 µg | 300 µg |
|---|---|---|
| Cocktail of 5 Antibodies | 1 x 150µg | 1 x 300µg |
Research areas
Metabolism >> Pathways and Processes >> Metabolic signaling pathways >> Energy transfer pathways >> Energy Metabolism
Metabolism >> Pathways and Processes >> Metabolic signaling pathways >> Carbohydrate metabolism
Metabolism >> Pathways and Processes >> Mitochondrial Metabolism >> Mitochondrial markers
Cancer >> Cancer Metabolism >> Metabolic signaling pathway >> Metabolism of carbohydrates
Kits/ Lysates/ Other >> Kits >> Cell Metabolism Kits >> Other Metabolism Assay
Signal Transduction >> Metabolism >> Mitochondrial
Signal Transduction >> Metabolism >> Energy Metabolism
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Western blot - MitoProfile® Pyruvate dehydrogenase (PDH) WB Antibody Cocktail (ab110416)
(enlarge)
Alternative names
- MSP02-150
Applications
Show applications key
Our Abpromise guarantee covers the use of ab110416 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use a concentration of 6 µg/ml. Suggested concentration.
MitoProfile® Pyruvate dehydrogenase (PDH) WB Antibody Cocktail images:
References for MitoProfile® Pyruvate dehydrogenase (PDH) WB Antibody Cocktail (ab110416)
This product has been referenced in:
- Elrod JW et al. Cyclophilin D controls mitochondrial pore-dependent Ca(2+) exchange, metabolic flexibility, and propensity for heart failure in mice. J Clin Invest 120:3680-7 (2010).Read more (PubMed: 20890047) »
- Quintana E et al. PDH E(1)beta deficiency with novel mutations in two patients with Leigh syndrome. J Inherit Metab Dis : (2009).Read more (PubMed: 19924563) »
See all 5 publications for this product
Publishing research using ab110416? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"