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Read our guarantee »Overview
Product name
MitoProfile® Total OXPHOS Human WB Antibody Cocktail
Assay type
Quantitative
Cross reactivity
Reacts with
Human
Product overview
ab110411 (MS601) contains 5 mAbs, one each against Complex I subunit NDUFB8 (ab110242/MS105), Complex II subunit 30kDa (ab14714/MS203), Complex III subunit Core 2 (ab14745/MS304), Complex IV subunit II (ab110258/MS405), and ATP synthase subunit alpha (ab14748/MS507) as an optimized premixed cocktail. The cocktail is suitable for Western Blotting analysis of the relative levels of the 5 OXPHOS complexes in mitochondrial preparations from human sources. Human heart mitochondria (50ug) are also included as a Western Blotting control.
Altered levels of assembly can arise from mutations in individual subunits, mutations in assembly factors for the complex(es), mtDNA depletion or as a result of physiological and or pathological changes e.g. hormone treatment, exercise, diet or oxidative stress.
The mAbs in the cocktail were chosen because they are against a subunit that is labile when its complex is not assembled. Moreover, the combination is readily resolved in SDS-PAGE when the appropriate gel conditions are used (see protocols).
Cocktail Antibodies:
Mouse monoclonal [20E9DH10C12] to (C-I-20) NDUFB8 (ab110242):
Amount: 50ug
Working concentration: 1 ug/ml
Mouse monoclonal [21A11AE7] to (C-II-30) SDHB (ab14714):
Amount: 250 ug
Working concentration: 5 ug/ml
Mouse monoclonal [13G12AF12BB11] to (C-III-Core 2) UQCRC2 (ab14745) :
Amount: 5 ug
Working concentration: 0.1 ug/ml
Mouse monoclonal [12C4F12] to (C-IV-II) Cytochrome C oxidase subunit II (ab110258):
Amount: 50ug
Working concentration: 1 ug/ml
Mouse monoclonal [15H4C4] to (C-V-alpha) ATP5A (ab14748):
Amount: 5 ug
Working concentration: 0.1 ug/ml
Notes
Store cocktail at 4°C and control at -80°C.
Tested applications
Properties
Storage instructions
Please see Notes section
| Components | 360 µg | 720 µg |
|---|---|---|
| Human Heart Mitochondria Western Blot Control | 1 x 50µg | 1 x 50µg |
| Premix Cocktail of 5 monoclonal antibodies | 1 x 360µg | 1 x 720µg |
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Western blot - MitoProfile® Total OXPHOS Human WB Antibody Cocktail (ab110411)
(enlarge)
Alternative names
- MS601-360
Applications
Show applications key
Our Abpromise guarantee covers the use of ab110411 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use a concentration of 7.2 µg/ml. The antibody cocktail (1.5 mg/mL) should be diluted 208x to a final working concentration of 7.2 µg/mL for Western blotting.
MitoProfile® Total OXPHOS Human WB Antibody Cocktail images:
Western blot - MitoProfile® Total OXPHOS Human WB Antibody Cocktail (ab110411)
Each lane contains 10 µg of mitochondria isolated from MRC5 fibroblasts.
ab110411(MS601) was used to label lane 6, and the individual antibodies
comprising the cocktail were used for lanes 1-5.
References for MitoProfile® Total OXPHOS Human WB Antibody Cocktail (ab110411)
This product has been referenced in:
- Hamilton RT et al. Hippocampal responsiveness to 17ß-estradiol and equol after long-term ovariectomy: implication for a therapeutic window of opportunity. Brain Res 1379:11-22 (2011).Read more (PubMed: 21241683) »
- Panov AV et al. Metabolic and functional differences between brain and spinal cord mitochondria underlie different predisposition to pathology. Am J Physiol Regul Integr Comp Physiol 300:R844-54 (2011).Read more (PubMed: 21248309) »
See all 64 publications for this product
Publishing research using ab110411? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"