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Read our guarantee »Overview
Product name
MitoProfile® Total OXPHOS Rodent WB Antibody Cocktail
Assay type
Quantitative
Cross reactivity
Reacts with
Mouse, Rat, Cow, Human
Product overview
ab110413 (MS604) contains 5 mAbs, one each against CI subunit NDUFB8 ab110242(MS105), CII-30kDa ab14714(MS203), CIII-Core protein 2 ab14745(MS304) CIV subunit I ab14705(MS404) and CV alpha subunit ab14748(MS507) as an optimized premixed cocktail. The kit is suitable for Western Blotting analysis of the relative levels of the 5 OXPHOS complexes in mitochondrial preparations from mouse, rat, human, or bovine sources.
Altered levels of assembly can arise from mutations in individual subunits, mutations in assembly factors for the complex(es), mtDNA depletion or as a result of physiological and or pathological changes e.g. hormone treatment, exercise, diet or oxidative stress.
The mAbs in the cocktail were chosen because they are against a subunit that is labile when its complex is not assembled. Moreover, the combination is readily resolved in SDS-PAGE when the appropriate gel conditions are used (see protocols). Note: Mouse tissue samples can easily be contaminated with antibodies from the animal's blood. To avoid such background bands, use ab110413 in conjunction with a secondary against native antibodies. Also, COXI is a highly hydrophobic protein and appears as a broad band at ~35 kDa (not at its true molecular weight at 57 kDa). It is very sensitive to heating. Therefore, the samples, including the positive control, should not be heated over 50°C before loaded on the gel.
Cocktail Antibodies:
Mouse monoclonal [20E9DH10C12] to (C-I-20) NDUFB8 (ab110242):
Amount: 100 µg
Working Concentration: 2 µg/ml.
Mouse monoclonal [21A11AE7] to (C-II-30) SDHB (ab14714):
Amount: 125 µg
Working Concentration: 2.5 µg/ml.
Mouse monoclonal [13G12AF12BB11] to (C-III-Core 2) UQCRC2 (ab14745):
Amount: 25 µg
Working Concentration: 0.5 µg/ml.
Mouse monoclonal [1D6E1A8] to (C-IV-I) MTCO1 - Mitochondrial Marker(ab14705):
Amount: 25 µg
Working Concentration: 0.5 µg/ml.
Mouse monoclonal [15H4C4] to (C-V-a) ATP5A (ab14748):
Amount: 25 µg
Working Concentration: 0.5 µg/ml.
Notes
Store the antibody cocktail at 4°C and the control sample at -80°C.
Tested applications
Properties
Storage instructions
Please see Notes section
| Components | 300 µg | 600 µg |
|---|---|---|
| Cocktail of 5 Antibodies | 1 x 300µg | 1 x 600µg |
| Rat Heart Mitochondria Western Blot Control | 1 x 50µg | 1 x 600µg |
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Western blot - MitoProfile® Total OXPHOS Rodent WB Antibody Cocktail (ab110413)
(enlarge)
Alternative names
- MS604-300
Applications
Show applications key
Our Abpromise guarantee covers the use of ab110413 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use a concentration of 6 µg/ml. The antibody cocktail (1.5 mg/mL) should be diluted 250x to a final working concentration of 6.0 µg/mL for Western blotting
MitoProfile® Total OXPHOS Rodent WB Antibody Cocktail images:
Western blot - MitoProfile® Total OXPHOS Rodent WB Antibody Cocktail (ab110413)
Figure 1. Rat liver mitochondria labeled with ab110413(MS604). The sample in lane 1 was kept at room temperature, whereas the remaining three samples were heated to 37°C, 50°C, and 100°C, respectively.
References for MitoProfile® Total OXPHOS Rodent WB Antibody Cocktail (ab110413)
This product has been referenced in:
- Faerber G et al. Induction of heart failure by minimally invasive aortic constriction in mice: reduced peroxisome proliferator-activated receptor ? coactivator levels and mitochondrial dysfunction. J Thorac Cardiovasc Surg 141:492-500, 500.e1 (2011).Read more (PubMed: 20447656) »
- Hoy AJ et al. Adipose triglyceride lipase-null mice are resistant to high-fat diet-induced insulin resistance despite reduced energy expenditure and ectopic lipid accumulation. Endocrinology 152:48-58 (2011).Read more (PubMed: 21106876) »
See all 30 publications for this product
Publishing research using ab110413? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"