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Product name
Mitochondria Isolation Kit for Cultured Cells
See all Mitochondria Isolation products (4) ...
Assay type
Quantitative
Cross reactivity
Reacts with
Human, a wide range of other species
Product overview
ab110170 contains sufficient reagents for up to 20 benchtop mitochondria isolations from human cultured cells. Isolated mitochondria can be analyzed by Western Blotting, Immunocapture, or Activity assay.
Available with a dounce homogenizer as kit ab110170 (MS853).
Tested applications
Functional Studiesmore details
Properties
Storage instructions
Store at +4°C.
| Components | 1 kit |
|---|---|
| Reagent A | 1 x 50ml |
| Reagent B | 1 x 50ml |
| Reagent C | 1 x 10ml |
Research areas
Kits/ Lysates/ Other >> Kits >> Molecular Biology Kits >> Cell Fractionation Kits
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Western blot - Mitochondria Isolation Kit for Cultured Cells (ab110170)
(enlarge)
Alternative names
- MS850
- MS851
- MS852
- MS853
Applications
Show applications key
Our Abpromise guarantee covers the use of ab110170 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Functional Studies
FuncSFuncS
Mitochondria Isolation Kit for Cultured Cells images:
Western blot - Mitochondria Isolation Kit for Cultured Cells (ab110170)
Isolated mitochondria show enriched signal when compared to the whole cell extract. In lane 1, MRC5 mitochondria isolated with Abcam's ab110170 (MS852) Mitochondria Isolation Kit for Cultured Cells were loaded at 20 µg. In lanes 2 and 3, post-spin supernatant (SN) and whole cell extract were loaded at 20 µg.
Protocols
References for Mitochondria Isolation Kit for Cultured Cells (ab110170)
This product has been referenced in:
- McGreal RS et al. aB-crystallin/sHSP protects cytochrome c and mitochondrial function against oxidative stress in sens and retinal cells. Biochim Biophys Acta : (2012).Read more (PubMed: 22521365) »
- Bernier M et al. Negative Regulation of STAT3 Protein-mediated Cellular Respiration by SIRT1 Protein. J Biol Chem 286:19270-9 (2011). Purification; Mouse.Read more (PubMed: 21467030) »
See all 5 publications for this product
Publishing research using ab110170? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"