(MS852)
Mitochondria Isolation Kit for Cultured Cells (ab110170)
Overview
- Product nameMitochondria Isolation Kit for Cultured CellsSee all Mitochondria Isolation kits ...
- Assay typeQuantitative
- Species reactivityReacts with: Human, a wide range of other species
- Product overview
ab110170 contains sufficient reagents for up to 20 benchtop mitochondria isolations from human cultured cells. Isolated mitochondria can be analyzed by Western Blotting, Immunocapture, or Activity assay.
Available with a dounce homogenizer as kit ab110170 (MS853).
- Tested applicationsFunctional Studies more details
Properties
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Components 1 kit Reagent A 1 x 50ml Reagent B 1 x 50ml Reagent C 1 x 10ml - Research Areas
Applications
Our Abpromise guarantee covers the use of ab110170 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| Functional Studies | FuncS |
Mitochondria Isolation Kit for Cultured Cells images
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Western blot - Mitochondria Isolation Kit for Cultured Cells (ab110170)Isolated mitochondria show enriched signal when compared to the whole cell extract. In lane 1, MRC5 mitochondria isolated with Abcam's ab110170 (MS852) Mitochondria Isolation Kit for Cultured Cells were loaded at 20 µg. In lanes 2 and 3, post-spin supernatant (SN) and whole cell extract were loaded at 20 µg.
Protocols
References for Mitochondria Isolation Kit for Cultured Cells (ab110170)
This product has been referenced in:
- McGreal RS et al. aB-crystallin/sHSP protects cytochrome c and mitochondrial function against oxidative stress in sens and retinal cells. Biochim Biophys Acta : (2012). Read more (PubMed: 22521365) »
- Greenlee H et al. Lack of effect of coenzyme q10 on doxorubicin cytotoxicity in breast cancer cell cultures. Integr Cancer Ther 11:243-50 (2012). Read more (PubMed: 22544232) »
