• Product nameMitochondria Isolation Kit for Cultured Cells
    See all Mitochondria Isolation kits
  • Assay typeQuantitative
  • Species reactivity
    Reacts with: Human, a wide range of other species
  • Product overview

    ab110170 contains sufficient reagents for up to 20 benchtop mitochondria isolations from mammalian cultured cells. Isolated mitochondria can be analyzed by Western Blotting, Immunocapture, or Activity assay.


    Available with a dounce homogenizer as kit ab110171.


  • Tested applicationsSuitable for: Functional Studiesmore details



    Our Abpromise guarantee covers the use of ab110170 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    Functional Studies Use at an assay dependent dilution.

    Mitochondria Isolation Kit for Cultured Cells images

    • Isolated mitochondria show enriched signal when compared to the whole cell extract. In lane 1, MRC5 mitochondria isolated with Abcam's ab110170 (MS852) Mitochondria Isolation Kit for Cultured Cells were loaded at 20 µg. In lanes 2 and 3, post-spin supernatant (SN) and whole cell extract were loaded at 20 µg.


    References for Mitochondria Isolation Kit for Cultured Cells (ab110170)

    This product has been referenced in:
    • Gan L  et al. a-MSH and Foxc2 promote fatty acid oxidation through C/EBPß negative transcription in mice adipose tissue. Sci Rep 6:36661 (2016). Mouse . Read more (PubMed: 27819350) »
    • Liu B  et al. IL-33/ST2 signaling excites sensory neurons and mediates itch response in a mouse model of poison ivy contact allergy. Proc Natl Acad Sci U S A 113:E7572-E7579 (2016). Read more (PubMed: 27821781) »

    See all 15 Publications for this product

    Product Wall

    The level of contamination depends on the quality of the sample. To minimize contamination, I suggest to perform the isolation of freshly isolated/harvested cells. Any freeze/thaw step of the cell pellet will not yield a good preparation. However, ther...

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    The mitotox kits will work with mitochondria from any human tissue. Therefore it will work with mitochondria from human PBMCs.

    To isolate mitochondria from human PBMCs I suggest the customer to use ab110170 (Mitochondria Isolation Kit f...

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    You can use the leftover reagents of kit ab110170 with tissues. However, you will need to follow the tissue protocol to get the best outcome.

    Note, we have a specific Mitochondrial isolation kit for tissue: ab110168.

    Large clearance pestle (A)[clearance 0.0030-0.0050 in] is used for the initial sample reduction, such as tissue prep;

    Small clearance pestle (B) [0.0005-0.0025 in] is used to form the final homogenate.

    When prepare cell homogenate...

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    The procedure can accomodate approximately 20 benchtop mitochondria isolations, assuming about 4x10^7 cells per isolation are used. These amounts can be scaled up or down accordingly, as long as the cells are resuspended to 5 mg/ml in Reagent A, a...

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    ab109719 is not suitable for this purpose, this kit is based on detergent fractionation of cellular proteins into three fractions. RNA distribution was not tested with this kit.

    Mitochondrial isolation kits (ab110168 – ab1101...

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    I can confirm that the mitochondria isolated using this kit can be used in other biological assays.
    Please check protocol booklet page number 2 for expected yield based on cell line:
    Sample / Starting Material / Expected Yield
    MRC-5 / 4 – ...

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    You will want to use either ab110170 or ab110171 (which includes the homogenizer), as these are optimized for isolating fractions from cultured cells versus tissue samples. The supernatant from SPIN2 is the cytosolic fraction, though it might be contam...

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    Red blood cells do not contain mitochondria so PBMC isolation is recommended.

    Ab110170 can be used to make all of the fractions you need. After the Spin 2 step in the protocol, the supernatant is the cytosolic fraction. The mitochondrial pellet will be largely intact mitochondria. In order to rupture the mitochondria, you can us...

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