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Product name
Mitochondria Isolation Kit for Cultured Cells (with Dounce Homogenizer)
See all Mitochondria Isolation products (4) ...
Assay type
Quantitative
Cross reactivity
Reacts with
Human, a wide range of other species
Product overview
ab110171 (MS853) contains sufficient reagents for up to 20 benchtop mitochondria isolations from human cultured cells. Isolated mitochondria can be analyzed by Western Blotting, Immunocapture, or Activity assay.
Tested applications
Functional Studiesmore details
Properties
Storage instructions
Store at +4°C.
| Components | 1 kit |
|---|---|
| Dounce Homogenizer (2 ml size) | 1 unit |
| Reagent A | 1 x 50ml |
| Reagent B | 1 x 50ml |
| Reagent C | 1 x 10ml |
Research areas
Kits/ Lysates/ Other >> Kits >> Molecular Biology Kits >> Cell Fractionation Kits
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Western blot - Mitochondria Isolation Kit for Cultured Cells (with Dounce Homogenizer) (ab110171)
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Alternative names
- MS850
- MS851
- MS852
- MS853
Applications
Show applications key
Our Abpromise guarantee covers the use of ab110171 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
FuncS. Mitochondria isolations from cultured cells.
Mitochondria Isolation Kit for Cultured Cells (with Dounce Homogenizer) images:
Western blot - Mitochondria Isolation Kit for Cultured Cells (with Dounce Homogenizer) (ab110171)
Isolated mitochondria show enriched signal when compared to the whole cell extract. In lane 1, MRC5 mitochondria isolated with Abcam's ab110171/MS853 Mitochondria Isolation Kit for Cultured Cells (with Dounce Homogenizer) were loaded at 20 µg. In lanes 2 and 3, post-spin supernatant (SN) and whole cell extract were loaded at 20 µg.
Protocols
References for Mitochondria Isolation Kit for Cultured Cells (with Dounce Homogenizer) (ab110171)
This product has been referenced in:
- Hofmann AD et al. OXPHOS Supercomplexes as a Hallmark of the Mitochondrial Phenotype of Adipogenic Differentiated Human MSCs. PLoS One 7:e35160 (2012).Read more (PubMed: 22523573) »
- Potula R et al. Methamphetamine causes mitrochondrial oxidative damage in human T lymphocytes leading to functional impairment. J Immunol 185:2867-76 (2010).Read more (PubMed: 20668216) »
See all 4 publications for this product
Publishing research using ab110171? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"