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Product name
Mitochondria Isolation Kit for Tissue
See all Mitochondria Isolation products (4) ...
Sample type
Tissue
Assay type
Quantitative
Product overview
ab110168 (MS850) contains sufficient reagents for up to 10 benchtop mitochondria isolations from hard or soft tissues. Isolated mitochondria can be analyzed by Western Blotting, Immunocapture, or Activity assay.
Notes
Tested tissues: brain, liver and heart
Tested applications
Functional Studiesmore details
Properties
Storage instructions
Store at +4°C.
| Components | 1 kit |
|---|---|
| Isolation Buffer | 1 x 100ml |
| Wash Buffer | 1 x 30ml |
Research areas
Kits/ Lysates/ Other >> Kits >> Molecular Biology Kits >> Cell Fractionation Kits
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Western blot - Mitochondria Isolation Kit for Tissue (ab110168)
(enlarge)
Alternative names
- MS850
- MS851
- MS852
- MS853
Applications
Show applications key
Our Abpromise guarantee covers the use of ab110168 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Functional Studies
FuncSFuncS
Mitochondria Isolation Kit for Tissue images:
Western blot - Mitochondria Isolation Kit for Tissue (ab110168)
Rat liver mitochondria were isolated using Abcam's ab110169/MS851 Mitochondria Isolation Kit for Tissue (with Dounce Homogenizer). 2 µg (lane 1) and 10 µg (lane 2) of mitochondra were tested with Abcam's ab110413/MS604 (MitoProfile® Total OXPHOS Rodent WB Antibody Cocktail). Signal strength higher compared to homogenate at respective amounts, see lane 3 and 4.
Protocols
References for Mitochondria Isolation Kit for Tissue (ab110168)
This product has been referenced in:
- Olga Boyd Nectarine promotes longevity in Drosophila melanogaster Free Radical Biology and Medicine 50:1669-1678 (2011).Read more (PubMed: ) »
- Olga Boyd Nectarine promotes longevity in Drosophila melanogaster Free Radical Biology and Medicine 50:1669-1678 (2011).Read more (PubMed: ) »
See all 5 publications for this product
Publishing research using ab110168? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"