Overview

  • Product name
    Mitochondria Isolation Kit for Tissue
    See all Mitochondria Isolation kits
  • Sample type
    Tissue
  • Assay type
    Quantitative
  • Product overview

    ab110168 (MS850) contains sufficient reagents for up to 10 benchtop mitochondria isolations from hard or soft tissues. Isolated mitochondria can be analyzed by Western Blotting, Immunocapture, or Activity assay.

  • Notes

    Tested tissues: brain, liver and heart

  • Tested applications
    Suitable for: Functional Studiesmore details

Properties

Applications

Our Abpromise guarantee covers the use of ab110168 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Functional Studies Use at an assay dependent dilution.

Images

  • Rat liver mitochondria were isolated using Abcam's ab110169/MS851 Mitochondria Isolation Kit for Tissue (with Dounce Homogenizer). 2 µg (lane 1) and 10 µg (lane 2) of mitochondria were tested with Abcam's ab110413/MS604 (MitoProfile® Total OXPHOS Rodent WB Antibody Cocktail). Mitochondria are enriched and so Western blot band intensity of mitochondrial proteins is greater in these mitochondrial preparations when compared to homogenate at respective amounts, see lane 3 and 4

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Protocols

References

This product has been referenced in:
  • Gan L  et al. a-MSH and Foxc2 promote fatty acid oxidation through C/EBPß negative transcription in mice adipose tissue. Sci Rep 6:36661 (2016). Mouse . Read more (PubMed: 27819350) »
  • Huang Y  et al. Perineural dexmedetomidine attenuates inflammation in rat sciatic nerve via the NF-?B pathway. Int J Mol Sci 15:4049-59 (2014). Read more (PubMed: 24663080) »

See all 7 Publications for this product

Customer reviews and Q&As

I can confirm our Mitochondria Isolation Kit for Tissue (ab110168) is suitable to use with frozen tissue. If using frozen tissues, the tissue should be flash frozen and stored at least at -80ºC.

Abreviews
I have used this kit to isolate mitochondria from Drosophila melanogaster and Hogna lenta. I obtained over 50 μg protein in the mitochondria fraction from a dozen flies and the red eye pigment was removed in the first centrifugation step. I was also pleased to discover that the cytosolic fraction obtained is far superior for use in proteasome activity assays with fluorescent substrates (Suc-LLVY-AMC) than the whole lysate which I had used previously.
Username

Erin Munkácsy

Verified customer

Submitted Aug 07 2017

ab110168 can be used with 20-30 mg tissues however the mitochondrial yield would be 10-100 ug. Please check the downstream application whether this amount would be suitable.
The homogenization protocol we have provided is for 300-500 mg tissue so ...

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Samples fixed in ethanol are not compatible with ab110168. Ethanol fixes/precipitates proteins, so the samples are no longer suitable for organelle isolation.


1) Yes, the mitochondrial isoaltion kit is suitable for use with all kits listed.

2) Suspend the prepared mitochondria to 5.5 mg/mL as described in the attached document and follow from step 5 (adding detergent to mitochondia) to generat...

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For a protease inhibitor to use with the Mitochondria Isolation Kit for Tissue, ab110168, we recommend ab201116.

I can confirm the the mitochondria prepared with ab110168 can be frozen and stored in -80 C to be compatible for subsequent analysis using ab109721 kit. However, we cannot guarantee all other mitochondria activity kits available in Abcam catalog can be...

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Ab110168 should work perfectly well with mouse peripheral nerve tissue, provided that the quality of the tissue is good.

This kit is not antibody based and therefore it can work with any species.

I hope this information is he...

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As we discussed over the phone, I have contacted the lab at Mitosciences to get their input into this query. The mouse brain mitochondrial lysate has been prepared using standard mitochondrial isolation procedures. These procedures separate t...

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Thank you for contacting us at abcam. Please see below under each question the answers.



Questions for ab110170:

1. Step 3 of the protocol states: Resuspend the cells to 5 mg/ml in Reagent A in a 2-ml microtube. What does ...

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1-10 of 41 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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