Overview

  • Product nameMitochondria Isolation Kit for TissueSee all Mitochondria Isolation kits ...
  • Sample type
    Tissue
  • Assay typeQuantitative
  • Product overview

    ab110168 (MS850) contains sufficient reagents for up to 10 benchtop mitochondria isolations from hard or soft tissues. Isolated mitochondria can be analyzed by Western Blotting, Immunocapture, or Activity assay.

  • Notes

    Tested tissues: brain, liver and heart

  • Tested applicationsFunctional Studiesmore details

Properties

    Applications

    Our Abpromise guarantee covers the use of ab110168 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    Functional Studies Use at an assay dependent dilution.

    Mitochondria Isolation Kit for Tissue images

    • Rat liver mitochondria were isolated using Abcam's ab110169/MS851 Mitochondria Isolation Kit for Tissue (with Dounce Homogenizer). 2 µg (lane 1) and 10 µg (lane 2) of mitochondria were tested with Abcam's ab110413/MS604 (MitoProfile® Total OXPHOS Rodent WB Antibody Cocktail). Mitochondria are enriched and so Western blot band intensity of mitochondrial proteins is greater in these mitochondrial preparations when compared to homogenate at respective amounts, see lane 3 and 4

      .

    Protocols

    References for Mitochondria Isolation Kit for Tissue (ab110168)

    This product has been referenced in:
    • Huang Y  et al. Perineural dexmedetomidine attenuates inflammation in rat sciatic nerve via the NF-?B pathway. Int J Mol Sci 15:4049-59 (2014). Read more (PubMed: 24663080) »
    • Cho KI  et al. Distinct and atypical intrinsic and extrinsic cell death pathways between photoreceptor cell types upon specific ablation of Ranbp2 in cone photoreceptors. PLoS Genet 9:e1003555 (2013). Read more (PubMed: 23818861) »

    See all 6 Publications for this product

    Product Wall

    I can confirm the the mitochondria prepared with ab110168 can be frozen and stored in -80 C to be compatible for subsequent analysis using ab109721 kit. However, we cannot guarantee all other mitochondria activity kits available in Abcam catalog can be...

    Read More


    Ab110168 should work perfectly well with mouse peripheral nerve tissue, provided that the quality of the tissue is good.

    This kit is not antibody based and therefore it can work with any species.

    I hope this information is he...

    Read More



    As we discussed over the phone, I have contacted the lab at Mitosciences to get their input into this query. The mouse brain mitochondrial lysate has been prepared using standard mitochondrial isolation procedures. These procedures separate t...

    Read More

    Thank you for contacting us at abcam. Please see below under each question the answers.



    Questions for ab110170:

    1. Step 3 of the protocol states: Resuspend the cells to 5 mg/ml in Reagent A in a 2-ml microtube. What does ...

    Read More

    Thank you for contacting us.

    It is the amount of tissue. e.g. from 3-5 grams of liver tissue ˜2-4mg of mitochondrial yield can be expected.

    I hope this information is helpful to you. Please do not hesitate to contact us if you need an...

    Read More

    Thank you for contacting us.

    Please check the Table 1 of protocol for answers;

    http://www.abcam.com/ps/products/110/ab110168/documents/ab110168%20Mitochondria%20Isolation%20Kit%20for%20Tissue%20booklet%20%20(Website).pdf

    I ho...

    Read More

    Thank you for your enquiry.

    I can confirm that this kit can be used for frozen skeletal muscle of mouse. Please follow the "Heart" protocol. It will be key to mince finely the tissue with a razor before using the dounce, as skeletal muscle a...

    Read More

    Vielen Dank für Ihre Antwort.

    Es tut mir sehr leid dass ich in diesem Fall nicht mehr helfen konnte.

    Ich habe ein wenig nach vorhandener Literatur gesucht zur Lokalisation von GADPH als auch alpha-Tubulin.

    Ich denke Sie ...

    Read More

    Thank you for contacting us.

    Were you referring to ab110168, the Mitochondria Isolation Kit for Tissue? Tje ab11068 is an antibody, so I assume you're talking about this kit.

    For ab110170, the cell pellet is frozen in step 2 of the...

    Read More

    Thank you for contacting us.

    Samples may be prepared as purified mitochondria, crudemitochondria. In most cases whole tissue or cell lysates aresuitable but these may require some sample optimization. Mitochondria samples can be prepared bys...

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"