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Product name
Mitochondrial Aldehyde Dehydrogenase (ALDH2) Activity Assay Kit
Precision
| Sample | n | Mean | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 5% |
| Sample | n | Mean | SD | CV% |
|---|---|---|---|---|
| 1 | 4 | 12% |
Tests
1 x 96 test
Sample type
Cell culture extracts, Tissue Extracts
Assay type
Enzyme activity
Range
60 µg/ml - 250 µg/ml
Cross reactivity
Reacts with
Mouse, Rat, Cow, Dog, Human
Product overview
ab115348 is used to determine mitochondrial aldehyde dehydrogenase activity (ALDH2) in a sample. The enzyme is captured within the wells of the microplate and activity is determined by following the production of NADH in the following ALDH2 catalyzed reaction:
acetaldehyde + NAD+ -> acid + NADH
The generation of NADH is coupled to the 1:1 reduction of a reporter dye to yield a colored (yellow) reaction product whose concentration can be monitored by measuring the increase in absorbance at 450 nm (Dye molar extinction coefficient - 37000 M-1cm-1). ab115348 immunocaptures in each well only native ALDH2 from the chosen sample; this removes all other enzymes, including unrelated aldehyde dehydrogenases.
Notes
Reagent dye, Coupler and NAD+ are shipped lyophilized. Before use rehydrate by adding 0.25 mL pure water to each tube and vortex each tube thoroughly to dissolve. After hydration unused amounts of these three materials should be stored at -80°C. Store all other components store at 4°C. This kit is stable for 6 months from receipt.
Tested applications
Functional Studiesmore details
Properties
Storage instructions
Please see Notes section
| Components | Identifier | 1 x 96 tests |
|---|---|---|
| 100X acetaldehyde (2.5 M) | 1 x 0.25ml | |
| 100X Coupler | 1 x 0.25ml | |
| 100X NAD+ (0.1M) | 1 x 0.25ml | |
| 100X Reagent Dye | 1 x 0.25ml | |
| 10X Blocking Buffer | P/N 8209802 | 1 x 6ml |
| 1X Base Buffer | 1 x 24ml | |
| 1X Extraction Buffer | P/N 8201093 | 1 x 15ml |
| 20X Buffer | P/N 8203013 | 1 x 20ml |
| 96 well microplate (12 strips) | 1 unit |
Research areas
Metabolism >> Pathways and Processes >> Metabolic signaling pathways >> Alcohol metabolism
Signal Transduction >> Metabolism >> Alcohol metabolism
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Functional Studies - Mitochondrial Aldehyde Dehydrogenase (ALDH2) Activity Assay Kit (ab115348)
(enlarge)
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Functional Studies - Mitochondrial Aldehyde Dehydrogenase (ALDH2) Activity Assay Kit (ab115348)
(enlarge)
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Functional Studies - Mitochondrial Aldehyde Dehydrogenase (ALDH2) Activity Assay Kit (ab115348)
(enlarge)
Alternative names
- MS953
Applications
Show applications key
Our Abpromise guarantee covers the use of ab115348 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
FuncS
Mitochondrial Aldehyde Dehydrogenase (ALDH2) Activity Assay Kit images:
Functional Studies - Mitochondrial Aldehyde Dehydrogenase (ALDH2) Activity Assay Kit (ab115348)
Reference sample graph
Functional Studies - Mitochondrial Aldehyde Dehydrogenase (ALDH2) Activity Assay Kit (ab115348)
Reference Sample graph
Functional Studies - Mitochondrial Aldehyde Dehydrogenase (ALDH2) Activity Assay Kit (ab115348)
Determination of effect reactive species on ALDH2 activity.
The mitochondrial location of ALDH2 may increase the exposure of this enzyme to high levels of reactive oxygen and nitrogen species generated in mitochondria. ALDH2 has been shown to be specifically
inactivated by nitrative stress, particularly by modification or crosslinking of an active site cysteine. To demonstrate this rat liver homogenate was exposed in vitro to a dilution series of peroxynitirite at concentrations - 0, 25, 50, 100, 200, 400, 800, 1600 µM. After exposure the samples were extracted, diluted to 0.25 mg/mL and loaded into microplate wells in duplicate. After following the assay protocol as described above, the activity of each sample was measured and graphed against peroxynitrite exposure concentration.
The ALDH2 activity after exposure was reduced with an IC50approximately 210 µM.
Protocols
References for Mitochondrial Aldehyde Dehydrogenase (ALDH2) Activity Assay Kit (ab115348)
ab115348 has not yet been referenced specifically in any publications.
Publishing research using ab115348? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"