Overview

  • Product nameMitochondrial DNA Isolation Kit
  • Tests
    50 x 1 assay
  • Sample type
    Tissue, Adherent cells, Suspension cells
  • Assay typeDirect
  • Product overview

    Abcam's Mitochondrial DNA Isolation Kit provides convenient tools for isolating mtDNA from a variety of cells and tissues in high yield and purity, without contaminations from genomic DNA. The purified mtDNA can be used for a variety of studies such as enzyme manipulations, Southern blotting, cloning, PCR analysis, and amplifications
    Visit our FAQs page for tips and troubleshooting.

  • Notes

    Mitochondria are semi-autonomous organelles which functions in aging process, apoptosis, anti-HIV drugs, and cancers. Mitochondrial DNA (mtDNA) has a very high mutation rate and the mutations on mtDNA appear to be related to certain diseases such as diabetes, Alzheimer's disease, and muscle disorders. Isolation and quantification of mtDNA are often required to study the relationships between the diseases and mtDNA.

  • Tested applicationsFunctional Studiesmore details

Properties

  • RelevanceMitochondria are semiautonomous organelles which function in the aging process, apoptosis, anti-HIV drugs, and cancers. Mitochondrial DNA (mtDNA) has a very high mutation rate and the mutations on mtDNA appear to be related to certain diseases such as diabetes, Alzheimer’s disease, and muscle disorders.

Applications

Our Abpromise guarantee covers the use of ab65321 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Functional Studies Use at an assay dependent dilution.

Protocols

References for Mitochondrial DNA Isolation Kit (ab65321)

This product has been referenced in:
  • Martin SA  et al. Parallel high-throughput RNA interference screens identify PINK1 as a potential therapeutic target for the treatment of DNA mismatch repair-deficient cancers. Cancer Res 71:1836-48 (2011). Read more (PubMed: 21242281) »
  • Munwes I  et al. Variation in repeat length and heteroplasmy of the mitochondrial DNA control region along a core-edge gradient in the eastern spadefoot toad (Pelobates syriacus). Mol Ecol 20:2878-87 (2011). Read more (PubMed: 21645158) »

See all 3 Publications for this product

Product Wall

Thank you for contacting Abcam regarding ab65321.


Regarding this Mitochondrial DNA Isolation Kit:


1 - Expect to recover 5-20ug mtDNA

2- The most important step with this kit is to isolate a pure mitochondrial ...

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ab65321 is designed to isolate mitochondria first and then lyse the mitochondria to get the mtDNA. Thus, since you have a solution of DNA with genomic and mtDNA mixed in, then this kit is not designed to isolate the mtDNA specifically. One way the lab ...

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Both the Lysis buffer and Enzyme mix are available separately. Here are the product numbers:

Lysis Buffer - ab192492 - http://www.abcam.com/mitochondrial-lysis-buffer-ab192492.html

Enzyme Mix - ab186679 http://www.abcam.com/enzyme-b-m...

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Thank you for your enquiries regarding ab65321 Mitochondrial DNA Isolation Kit .

I have now obtained some further information from the laboratories.

As I thought, the Enzyme B composition is proprietary. However, I can confirm there ...

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I can confirm that the isolated mtDNA in the mitochondria isolated with this kit will be circular and will run at ˜15-20 kbs.

Vielen Dank für Ihre Geduld.

Leider ist diese Information ein Betriebsgeheimnis, doch das Labor ist gerne bereit, auf mehr spezielle Fragen einzugehen, wie zb. pH- Wert einzelner Reagenzien, oder Verwendung nach der Lyse.

Ich...

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Thank you for contacting us.

We recommend the 2 mL version of this homogenizer:

https://ca.vwr.com/store/catalog/product.jsp?product_id=4830627

I hope this information is helpful to you. Please do not hesitate to contact...

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Vielen Dank für Ihre Anfrage.
Leider bieten wir generell keine kostenlosen Proben zu Testzwecken an, da wir bei über 100.000Produkten gar nicht die logistischen Möglichkeiten haben, neben unserer eigentlichen Produktpallette Teströhrchen zu lagern...

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Gracias por contactarnos.

Tenemos un kit en el catálogo, el http://www.abcam.com/mitochondrial-dna-isolation-kit-ab65321.html, que podría en teoría usarse con tejido previamente fijado, siendo que la integridad del tejido...

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Thank you for your reply.

I have received the following information from the producer of this kit, which I hope will be helpful. they have provided some tips on preparation of the tissue:

'Tissues are much dirtier than cultured ce...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"