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Mitochondrial DNA Isolation Kit (ab65321)

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Overview

Product name

Mitochondrial DNA Isolation Kit

Product overview

Mitochondrial DNA (mtDNA) has a very high mutation rate and the mutations on mtDNA appear to be related to certain diseases such as diabetes, Alzheimer’s disease, and muscle disorders. Isolation and quantification of mtDNA are often required to study the relationships between the diseases and mtDNA. The Mitochondrial DNA Extraction Kit provides convenient tools for isolating mtDNA from a variety of cells and tissues in high yield and purity, without contaminations from genomic DNA. The purified mtDNA can be used for a variety of studies such as enzyme manipulations, Southern blotting, cloning, PCR analysis, and amplifications.

Notes

After opening the kit, store Enzyme Mix at –70oC. Store all other Buffers at 4oC.

Tested applications

Functional Studiesmore details

Properties

Storage instructions

After opening the kit, store Enzyme Mix at –70°C. Store all other Buffers at 4°C.

Components Identifier 50 tests
5X Cytosol Extraction BufferWM1 x 20ml
Enzyme Mix (lyophilized)Red1 vial
Mitochondrial Lysis BufferPurple1 x 1.8ml
TE BufferGreen1 x 1.5ml

Research areas

Kits/ Lysates/ Other >> Kits >> Molecular Biology Kits >> DNA Isolation Kits
Kits/ Lysates/ Other >> Tools and Reagents >> DNA

Relevance

Mitochondria are semiautonomous organelles which function in the aging process, apoptosis, anti-HIV drugs, and cancers. Mitochondrial DNA (mtDNA) has a very high mutation rate and the mutations on mtDNA appear to be related to certain diseases such as diabetes, Alzheimer’s disease, and muscle disorders.

Applications

Show applications key

Our Abpromise guarantee covers the use of ab65321 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

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    Functional Studies

     FuncS: Use at an assay d...Read more →

    FuncS: Use at an assay dependent dilution.

Protocols

  • Mitochondrial DNA Isolation Kit

    • References for Mitochondrial DNA Isolation Kit (ab65321)

    This product has been referenced in:

    • Martin SAet al. Parallel high-throughput RNA interference screens identify PINK1 as a potential therapeutic target for the treatment of DNA mismatch repair-deficient cancers. Cancer Res 71:1836-48 (2011).Read more (PubMed: 21242281) »
    • Martin SAet al. DNA polymerases as potential therapeutic targets for cancers deficient in the DNA mismatch repair proteins MSH2 or MLH1. Cancer Cell 17:235-48 (2010).Read more (PubMed: 20227038) »

    See all 2 publications for this product

    Publishing research using ab65321? Please let us know so that we can cite the reference in this datasheet

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"