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Overview

  • Product nameMitochondrial DNA Isolation Kit
  • Tests
    50 x 1 assay
  • Sample type
    Tissue, Adherent cells, Suspension cells
  • Assay typeDirect
  • Product overview

    Abcam's Mitochondrial DNA Isolation Kit provides convenient tools for isolating mtDNA from a variety of cells and tissues in high yield and purity, without contaminations from genomic DNA. The purified mtDNA can be used for a variety of studies such as enzyme manipulations, Southern blotting, cloning, PCR analysis, and amplifications
    Visit our FAQs page for tips and troubleshooting.

  • Notes

    Mitochondria are semi-autonomous organelles which functions in aging process, apoptosis, anti-HIV drugs, and cancers. Mitochondrial DNA (mtDNA) has a very high mutation rate and the mutations on mtDNA appear to be related to certain diseases such as diabetes, Alzheimer's disease, and muscle disorders. Isolation and quantification of mtDNA are often required to study the relationships between the diseases and mtDNA.

  • Tested applicationsFunctional Studies more details

Properties

  • RelevanceMitochondria are semiautonomous organelles which function in the aging process, apoptosis, anti-HIV drugs, and cancers. Mitochondrial DNA (mtDNA) has a very high mutation rate and the mutations on mtDNA appear to be related to certain diseases such as diabetes, Alzheimer’s disease, and muscle disorders.

    • Applications

    Our Abpromise guarantee covers the use of ab65321 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    Functional Studies FuncS

    Protocols

    References for Mitochondrial DNA Isolation Kit (ab65321)

    This product has been referenced in:
    • Martin SA  et al. Parallel high-throughput RNA interference screens identify PINK1 as a potential therapeutic target for the treatment of DNA mismatch repair-deficient cancers. Cancer Res 71:1836-48 (2011). Read more (PubMed: 21242281) »
    • Munwes I  et al. Variation in repeat length and heteroplasmy of the mitochondrial DNA control region along a core-edge gradient in the eastern spadefoot toad (Pelobates syriacus). Mol Ecol 20:2878-87 (2011). Read more (PubMed: 21645158) »

    See all 3 Publications for this product

    Publishing research using ab65321 ? Please let us know so that we can cite the reference in this datasheet

    Product Wall

    Submit an Abreview for ab65321 Submit a Question for ab65321

    Displaying 1 - 10 of 44 results for Abreviews and Q&A

    10
    Votes: Highest First

    Inquiry: Hi there, I have a question about the Mitochondrial DNA Isolation Kit (ab65321). For my application I need the isolated mtDNA to remain in the circular form - do you know if the mtDNA isolated by the kit remains circular?

    I can confirm that the isolated mtDNA in the mitochondria isolated with this kit will be circular and will run at ˜15-20 kbs.

    Is it possible to get some information about the composition of the Cytosol Extraction Buffer and the Mitochondrial Lysis Buffer of the Mitochondrial DNA isolation kit (65321)? Thank you very much in advance.

    Vielen Dank für Ihre Geduld.

    Leider ist diese Information ein Betriebsgeheimnis, doch das Labor ist gerne bereit, auf mehr spezielle Fragen einzugehen, wie zb. pH- Wert einzelner Reagenzien, oder Verwendung nach der Lyse.

    Ich ho...

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    I don't have a Dounce homogenizer. Which one do you recommend?

    Thank you for contacting us.

    We recommend the 2 mL version of this homogenizer:

    https://ca.vwr.com/store/catalog/product.jsp?product_id=4830627

    I hope this information is helpful to you. Please do not hesitate to contact us...

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    Ich bin auf der Suche nach einem mtDNA Isolations Kit für mein Forschungsprojekt. Da mir der Artikel ab65321 für meine Anwendungen passend erscheint, möchte ich Sie fragen ob es Ihnen möglich ist mir eventuell ein Test-Kit zukommen zu ...

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    Vielen Dank für Ihre Anfrage.
    Leider bieten wir generell keine kostenlosen Proben zu Testzwecken an, da wir bei über 100.000Produkten gar nicht die logistischen Möglichkeiten haben, neben unserer eigentlichen Produktpallette Teströhrchen zu lagern. H...

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    Buenos días,
    Estaríamos interesados en algún kit que nos permitiera obtener DNA
    mitocondrial a partir de tejido fijado en etanol.
    Dispondrían de algún producto que nos sirviese?
    Un saludo

    Gracias por contactarnos.

    Tenemos un kit en el catálogo, el http://www.abcam.com/mitochondrial-dna-isolation-kit-ab65321.html, que podría en teoría usarse con tejido previamente fijado, siendo que la integridad del tejido si...

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    Thank you for your response. I bought mtDNA Isolation kit (ab65321) in August 2012 . My sample is a very small fish and type of tissues is muscle. After mtDNA was isolated and analyted by Agarose gel, the result show no band mtDNA. I think mtDNA could not...

    Read More

    Thank you for your reply.

    I have received the following information from the producer of this kit, which I hope will be helpful. they have provided some tips on preparation of the tissue:

    'Tissues are much dirtier than cultured cells...

    Read More

    Dear. Sir or Madam

    I bougt mitochondrail DNA Isolation kit from your company. I try to isolate by using this kit but I could not isolate mitochondrail DNA . I would like to find your favor to suggest me how to lysis my sample, it is fish tissue...

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    Thanks for the query.


    Generally, tissue samplesare much dirtier than cultured cells and very sticky after homogenization. There will be genomic DNA contamination in the mtDNA prearation and mtDNA may get partially degraded by DNases.
    Read More

    Hi Abcam,

    I have a question in regards to the tissue type for use with the mitochondrial isolation kit.
    I am working with a critically endangered species so have limited choice of tissue available to me and no means of obtaining new tissue...

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    Thanks for your query.

    This is the reply I received from the lab regarding your questions:
    "This process is slightly complex.

    This kit by itself is compatible with cells and tissue samples.

    If your client is start...

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    Hi Abcam,

    I have a question in regards to the tissue type for use with the mitochondrial isolation kit.
    I am working with a critically endangered species so have limited choice of tissue available to me and no means of obtaining new tissue...

    Read More

    Thank you for your inquiry.

    I have to confirm that tissues in 70% Ethanol are not suitable for mitochondrial isolation but can be used for IHC staining instead.

    Fresh blood cells are a suitable material to start with. Ifyou areonly int...

    Read More

    Hello,
    Thank you for looking towards my query and helping me out. It was very helpful and informative for me. I would also like to know the cost for purchasing the kit, I was looking on website but I didn't found the cost for it. Please let me know a...

    Read More

    Thank you for your reply.

    I can try to help with this information, however, could you please first let me know which country you would be purchasing the kit from?

    I look forward to receiving your reply.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"