Mitochondrial DNA Isolation Kit (ab65321)
- Product nameMitochondrial DNA Isolation Kit
- Tests50 x 1 assay
- Sample typeTissue, Adherent cells, Suspension cells
- Assay typeDirect
- Product overview
Abcam's Mitochondrial DNA Isolation Kit provides convenient tools for isolating mtDNA from a variety of cells and tissues in high yield and purity, without contaminations from genomic DNA. The purified mtDNA can be used for a variety of studies such as enzyme manipulations, Southern blotting, cloning, PCR analysis, and amplifications
Visit our FAQs page for tips and troubleshooting.
Mitochondria are semi-autonomous organelles which functions in aging process, apoptosis, anti-HIV drugs, and cancers. Mitochondrial DNA (mtDNA) has a very high mutation rate and the mutations on mtDNA appear to be related to certain diseases such as diabetes, Alzheimer's disease, and muscle disorders. Isolation and quantification of mtDNA are often required to study the relationships between the diseases and mtDNA.
- Tested applicationsFunctional Studies more details
- Storage instructionsStore at -20°C. Please refer to protocols.
Components Identifier 50 tests 5X Cytosol Extraction Buffer 1 x 20ml Enzyme Mix (lyophilized) Red 1 vial Mitochondrial Lysis Buffer Purple 1 x 1.8ml TE Buffer 1 x 1.5ml
- Research Areas
Our Abpromise guarantee covers the use of ab65321 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
References for Mitochondrial DNA Isolation Kit (ab65321)
This product has been referenced in:
- Martin SA et al. Parallel high-throughput RNA interference screens identify PINK1 as a potential therapeutic target for the treatment of DNA mismatch repair-deficient cancers. Cancer Res 71:1836-48 (2011). Read more (PubMed: 21242281) »
- Munwes I et al. Variation in repeat length and heteroplasmy of the mitochondrial DNA control region along a core-edge gradient in the eastern spadefoot toad (Pelobates syriacus). Mol Ecol 20:2878-87 (2011). Read more (PubMed: 21645158) »