Recombinant Anti-PDK1 antibody [SP270] - C-terminal (ab227682)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [SP270] to PDK1 - C-terminal
- Suitable for: IHC-P
- Reacts with: Human
Overview
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Product name
Anti-PDK1 antibody [SP270] - C-terminal
See all PDK1 primary antibodies -
Description
Rabbit monoclonal [SP270] to PDK1 - C-terminal -
Host species
Rabbit -
Tested applications
Suitable for: IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- IHC-P: Human kidney, liver, stomach, heart, pancreas, testis and liver hepatocellular carcinoma tissues.
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General notes
This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.60
Preservative: 0.1% Sodium azide
Constituents: PBS, 1% BSA -
Concentration information loading...
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Purity
Protein A/G purified -
Purification notes
Purified from TCS by Protein A/G. -
Clonality
Monoclonal -
Clone number
SP270 -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab227682 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
1/100.
Boil tissue section in EDTA buffer, pH 8.0 for 10 minutes followed by cooling at room temperature for 20 minutes. Primary antibody incubation for 10 minutes at room temperature. |
Notes |
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IHC-P
1/100. Boil tissue section in EDTA buffer, pH 8.0 for 10 minutes followed by cooling at room temperature for 20 minutes. Primary antibody incubation for 10 minutes at room temperature. |
Target
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Function
Inhibits the mitochondrial pyruvate dehydrogenase complex by phosphorylation of the E1 alpha subunit, thus contributing to the regulation of glucose metabolism. -
Tissue specificity
Expressed predominantly in the heart. -
Sequence similarities
Belongs to the PDK/BCKDK protein kinase family.
Contains 1 histidine kinase domain. -
Cellular localization
Mitochondrion matrix. - Information by UniProt
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Database links
- Entrez Gene: 5163 Human
- Omim: 602524 Human
- SwissProt: Q15118 Human
- Unigene: 470633 Human
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Alternative names
- [Pyruvate dehydrogenase [lipoamide]] kinase isozyme 1, mitochondrial antibody
- HGNC:8809 antibody
- Mitochondrial pyruvate dehydrogenase kinase isoenzyme 1 antibody
see all
Images
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Formalin-fixed, paraffin-embedded human kidney tissue stained for PDK1 using ab227682 at 1/100 dilution in immunohistochemical analysis.
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Formalin-fixed, paraffin-embedded human liver tissue stained for PDK1 using ab227682 at 1/100 dilution in immunohistochemical analysis.
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Formalin-fixed, paraffin-embedded human liver hepatocellular carcinoma tissue stained for PDK1 using ab227682 at 1/100 dilution in immunohistochemical analysis.
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Formalin-fixed, paraffin-embedded human stomach tissue stained for PDK1 using ab227682 at 1/100 dilution in immunohistochemical analysis.
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Formalin-fixed, paraffin-embedded human heart tissue stained for PDK1 using ab227682 at 1/100 dilution in immunohistochemical analysis.
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Formalin-fixed, paraffin-embedded human pancreas tissue stained for PDK1 using ab227682 at 1/100 dilution in immunohistochemical analysis.
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Formalin-fixed, paraffin-embedded human testis tissue stained for PDK1 using ab227682 at 1/100 dilution in immunohistochemical analysis.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab227682 has been referenced in 1 publication.
- Sun J et al. Circ_0000284 facilitates the growth, metastasis and glycolysis of intrahepatic cholangiocarcinoma through miR-152-3p-mediated PDK1 expression. Histol Histopathol N/A:18544 (2022). PubMed: 36331285