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Mitochondrial Transmembrane Potential Apoptosis Detection Kit (ab65310)

Q&A (4)

Overview

  • Product nameMitochondrial Transmembrane Potential Apoptosis Detection KitSee all Mitochondrial Transmembrane Potential kits ...
  • Sample type
    Adherent cells, Suspension cells
  • Assay typeQuantitative
  • Assay time
    0h 30m
  • Product overview

    Abcam's Mitochondrial Transmembrane Potential Apoptosis Detection Kit provides a simple, fluorescent-based method for distinguishing between healthy and apoptotic cells by detecting the changes in the mitochondrial transmembrane potential. The kit utilizes MitoCapture™, a cationic dye that fluoresces differently in healthy vs apoptotic cells. In healthy cells, MitoCapture accumulates and aggregates in the mitocondria, giving off a bright red fluorescence. In apoptotic cells, MitoCapture cannot aggregate in the mitochondria due to the altered mitochondrial transmembrane potential, and thus it remains in the cytoplasm in its monomer form, fluorescing green. The fluorescent signals can be easily detected by fluorescence microscopy using a band-pass filter (detects FITC and rhodamine) or analyzed by flow cytometry using FITC channel for green monomers (Ex/Em = 488/530+ 30 nm) and (optional) PI channel for red aggregates (Em = 488/590+ 42 nm).
    Visit our FAQs page for tips and troubleshooting.

  • Notes

    Disruption of the mitochondrial transmembrane potential is one of the earliest intracellular events that occur following induction of apoptosis.

  • Tested applicationsFluorescence Microscopy, Flow Cyt, Functional Studies more details

Properties

  • RelevanceMitochondrial Transmembrane Potential is an important parameter of mitochondrial function used as an indicator of cell death. The collapse of the mitochondrial transmembrane potential coincides with the opening of the mitochondrial permeability transition pores, leading to the release of cytochrome c into the cytosol, which in turn triggers other downstream events in the apoptotic cascade.
    • Alternative names
        mitochondrial membrane potential

    Applications

    Our Abpromise guarantee covers the use of ab65310 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    Fluorescence Microscopy Fluorescence Microscopy
    Flow Cyt Flow Cyt
    Functional Studies FuncS

    Protocols

    References for Mitochondrial Transmembrane Potential Apoptosis Detection Kit (ab65310)

    ab65310 has not yet been referenced specifically in any publications.

    Publishing research using ab65310 ? Please let us know so that we can cite the reference in this datasheet

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    To detect the stained cells under the microscope, the protocol said [Place the cell suspension from Step 1.g on a glass slide. Cover the cells with a glass coverslip.]
    Well, the suspension from that step is 1 ml !
    How can I cover this big volume...

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    You are correct, 1 ml is too much, and the instructions are too vague. You can pipet just a portion of that cell suspension onto a slide, for instance 50 ul, then coverslip. That will be 50,000 cells if you start with one million. If you find that the cel...

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    I am going to have different samples to evaluate, do you recommend cells fixation for more stable sample preparation? if the answer is yes, is there any specific protocol or fixation solution you recommend to me to use?

    We assume this assay requires living cells, and that fixation before adding the dye will give unreliable results. However, we have not compared results from fixed and unfixed samples. The question is whether the fixation itself will alter the mitochondria...

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    Using this kit on adherent cells, which are detaching from the surface during the assay. The apoptosis treatment is a trypsin-like enzyme, and the cells are treated for 2 hours without FBS.

    Thank you very much for your call last week and for your patience while I have been in touch with the lab regarding your enquiry.

    The new kit ab65310 actually contains the same reagents that were previously included in the kit ab65309. Ab65310 i...

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    Thank you for your confirmation.
    Our customer still has doubt about the differences between ab65309 and ab65310.
    Because she thinks there should be some differences that's why abcam sells the same product name with different cat#.
    If there'...

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    Thank you for your reply.


    Please assure your customer that the two are indeed the same product. Once we have sold the remaining units of this kit under ab65309, all stock will be listed under ab65310, with the option to purchase the 25 or ...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"