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Mitochondrial Viability Stain (ab129732) is a fluorometric/colorimetric assay that allows determination of the metabolic capacity of live cells in high throughput format. It uses an indicator dye to measure oxidation-reduction reactions which principally occur in the mitochondria of live cells. When reduced by metabolically active cells the non-fluorescent dark blue dye becomes fluorescent pink with absorbance at 570nm and red-fluorescent properties (560±10Ex/590Em) at neutral pH. The dye can be measured in fluorescence or absorbance mode. However fluorescence mode measurement offers greater assay linearity, reproducibility, robustness and sensitivity.
|20x Mitochondrial viability stain||1 x 24ml|
Our Abpromise guarantee covers the use of ab129732 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Functional Studies||Use at an assay dependent concentration.|
ab129732 has not yet been referenced specifically in any publications.