Overview

  • Product name
    Anti-Mitochondrial p110 antibody [2G2]
  • Description
    Mouse monoclonal [2G2] to Mitochondrial p110
  • Tested applications
    Suitable for: ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Human, Monkey
  • Immunogen

    Recombinant full length protein (Human).

  • General notes


    p110 has an approximate isoelectric point of 6.5 and co-partitions with HSP-60 proteins during isolation of mitochondria from HeLa cells. Close examination of the staining pattern in HeLa and Fanconi’s anemia cells reveal differences in the morphology and organization of mitochondria in these two cell types. In HeLa cells, mitochondria appear as individual tubular compartments of variable length and are closely associated with vimentin filaments, particularly at the periphery of the nucleus. In Fanconi’s anemia cells, mitochondria have a filamentous shape and form an interconnected cytoplasmic reticulum running in parallel with both vimentin filaments and microtubules.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.08% Sodium Azide
    Constituents: PBS, pH 7.4
  • Concentration information loading...
  • Purity
    Protein G purified
  • Primary antibody notes
    p110 has an approximate isoelectric point of 6.5 and co-partitions with HSP-60 proteins during isolation of mitochondria from HeLa cells. Close examination of the staining pattern in HeLa and Fanconi’s anemia cells reveal differences in the morphology and organization of mitochondria in these two cell types. In HeLa cells, mitochondria appear as individual tubular compartments of variable length and are closely associated with vimentin filaments, particularly at the periphery of the nucleus. In Fanconi’s anemia cells, mitochondria have a filamentous shape and form an interconnected cytoplasmic reticulum running in parallel with both vimentin filaments and microtubules.
  • Clonality
    Monoclonal
  • Clone number
    2G2
  • Isotype
    IgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab10435 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent dilution.
WB Use a concentration of 2 - 10 µg/ml. Detects a band of approximately 110 kDa.

References

This product has been referenced in:
  • Paulin-Levasseur M  et al. The 2G2 antibody recognizes an acidic 110-kDa human mitochondrial protein. Histochem J 30:617-25 (1998). Read more (PubMed: 9870762) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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