Anti-Mitochondrial p110 antibody [2G2] (ab10435)
Overview
- Product nameAnti-Mitochondrial p110 antibody [2G2]
- DescriptionMouse monoclonal [2G2] to Mitochondrial p110
- Tested applicationsICC/IF, WB more details
- Species reactivityReacts with: Human, Monkey
- Immunogen
Recombinant full length protein (Human).
- General notes
p110 has an approximate isoelectric point of 6.5 and co-partitions with HSP-60 proteins during isolation of mitochondria from HeLa cells. Close examination of the staining pattern in HeLa and Fanconi’s anemia cells reveal differences in the morphology and organization of mitochondria in these two cell types. In HeLa cells, mitochondria appear as individual tubular compartments of variable length and are closely associated with vimentin filaments, particularly at the periphery of the nucleus. In Fanconi’s anemia cells, mitochondria have a filamentous shape and form an interconnected cytoplasmic reticulum running in parallel with both vimentin filaments and microtubules.
Properties
- FormLiquid
- Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
- Storage bufferPreservative: 0.08% Sodium Azide
Constituents: PBS, pH 7.4 -
Concentration information loading... - PurityProtein G purified
- Primary antibody notes p110 has an approximate isoelectric point of 6.5 and co-partitions with HSP-60 proteins during isolation of mitochondria from HeLa cells. Close examination of the staining pattern in HeLa and Fanconi’s anemia cells reveal differences in the morphology and organization of mitochondria in these two cell types. In HeLa cells, mitochondria appear as individual tubular compartments of variable length and are closely associated with vimentin filaments, particularly at the periphery of the nucleus. In Fanconi’s anemia cells, mitochondria have a filamentous shape and form an interconnected cytoplasmic reticulum running in parallel with both vimentin filaments and microtubules.
- Clonality Monoclonal
- Clone number2G2
- IsotypeIgG1
- Research Areas
Applications
Our Abpromise guarantee covers the use of ab10435 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| ICC/IF | ICC/IF: Use at an assay dependent dilution. |
| WB | WB: Use a concentration of 2 - 10 µg/ml. Detects a band of approximately 110 kDa. |
References for Anti-Mitochondrial p110 antibody [2G2] (ab10435)
This product has been referenced in:
- Paulin-Levasseur M et al. The 2G2 antibody recognizes an acidic 110-kDa human mitochondrial protein. Histochem J 30:617-25 (1998). Read more (PubMed: 9870762) »
