![Immunocytochemistry/ Immunofluorescence - Mitofilin antibody [2E4AD5 ] (ab110329) image](#Mitofilin-Primary-antibodies-ab110329-1.jpg)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Mitofilin antibody [2E4AD5 ] (ab110329) image](#Mitofilin-Primary-antibodies-ab110329-2.gif)
![Western blot - Mitofilin antibody [2E4AD5 ] (ab110329) image](#Mitofilin-Primary-antibodies-ab110329-3.jpg)
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Product name
Anti-Mitofilin antibody [2E4AD5]
See all Mitofilin products (5) ...
Description
Mouse monoclonal [2E4AD5] to Mitofilin
Tested applications
WB, ICC/IF, IHC-P, Flow Cyt, In-Cell ELISA, IPmore details
Cross reactivity
Reacts with
Mouse, Rat, Cow, Human
Immunogen
Human heart mitochondria.
Positive control
Human fibroblasts; Human cerebellum tissue; Isolated mitochondria from Human, Bovine, Rat and Mouse hearts, HepG2 lysate; HL60 cells.
Properties
Form
Liquid
Storage instructions
Store at +4°C. Do not freeze.
Storage buffer
Preservative: 0.02% Sodium azide
Constituent: HBS
Concentration
Concentration information loading...
Purity
>95% by SDS-PAGE
Purification notes
ab110329 was produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation.
Clonality
Monoclonal
Clone number
2E4AD5
Isotype
IgG1
Light chain type
kappa
Research areas
Metabolism >> Types of disease >> Cancer
Metabolism >> Pathways and Processes >> Mitochondrial Metabolism >> Mitochondrial markers
Cardiovascular >> Heart >> Cardiac metabolism
Signal Transduction >> Metabolism >> Mitochondrial
Applications
Show applications key
Our Abpromise guarantee covers the use of ab110329 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use a concentration of 2 µg/ml. Predicted molecular weight: 84 kDa.
ICC/IF: Use a concentration of 1 - 5 µg/ml.
IHC-P: 1/100. Perform heat mediated antigen retrieval via the microwave method before commencing with IHC staining protocol.
Flow Cyt: Use a concentration of 1 µg/ml.
In-Cell ELISA: Use a concentration of 4 µg/ml.
IP: Use at an assay dependent dilution.
Target
Cellular localization
Mitochondrion inner membrane.
Target information above from: UniProt accessionQ16891
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Cell proliferation inducing protein 52 antibody
- Cell proliferation-inducing gene 4/52 protein antibody
- HMP antibody
see all
Database links
- Entrez Gene: 508853 Cow
- Entrez Gene: 10989 Human
- Entrez Gene: 76614 Mouse
- Entrez Gene: 312444 Rat
- Omim: 600378 Human
- SwissProt: Q2NL19 Cow
- SwissProt: Q16891 Human
- SwissProt: Q8CAQ8 Mouse
see all
Anti-Mitofilin antibody [2E4AD5] images:
Immunocytochemistry/ Immunofluorescence - Mitofilin antibody [2E4AD5 ] (ab110329)
![Immunocytochemistry/ Immunofluorescence - Mitofilin antibody [2E4AD5 ] (ab110329)](/ps/datasheet/images/110/ab110329/Mitofilin-Primary-antibodies-ab110329-1.jpg)
Immunocytochemistry analysis using ab110329 at 5µg/ml staining Mitofilin in Cultured Human fibroblasts. (Fixed and permeabilized) followed by a fluorescent goat-anti-mouse IgG.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Mitofilin antibody [2E4AD5 ] (ab110329)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Mitofilin antibody [2E4AD5 ] (ab110329)](/ps/datasheet/images/110/ab110329/Mitofilin-Primary-antibodies-ab110329-2.gif)
Immunohistological analysis using ab110329 at 1/100 dilution staining Mitofilin in Human cerebellum tissue (Formalin-fixed, Paraffin-embedded).
Western blot - Mitofilin antibody [2E4AD5 ] (ab110329)
![Western blot - Mitofilin antibody [2E4AD5 ] (ab110329)](/ps/datasheet/images/110/ab110329/Mitofilin-Primary-antibodies-ab110329-3.jpg)
All lanes : Anti-Mitofilin antibody [2E4AD5] (ab110329) at 2 µg/ml
Lane 1 : Isolated mitochondria from Human heart at 5 µg
Lane 2 : Isolated mitochondria from Bovine heart at 1 µg
Lane 3 : Isolated mitochondria from Rat heart at 10 µg
Lane 4 : Isolated mitochondria from Mouse heart at 10 µg
Lane 5 : HepG2 cell lysate at 20 µg
Predicted band size : 84 kDa
Extra bands in the mouse sample (lane 4) are due to the reaction of the IgG-specific goat anti-mouse secondary antibody with residual mouse blood in the heart tissue, as it is very difficult to entirely remove the blood from these small organs.
Flow Cytometry - Mitofilin antibody [2E4AD5 ] (ab110329)
![Flow Cytometry - Mitofilin antibody [2E4AD5 ] (ab110329)](/ps/datasheet/images/110/ab110329/Mitofilin-Primary-antibodies-ab110329-4.gif)
Flow cytometric analysis using ab110329 at 1µg/ml staining Mitofilin in HL60 cells (blue). Isotype control antibody (red).
References for Anti-Mitofilin antibody [2E4AD5] (ab110329)
This product has been referenced in:
- Iyer S et al. Mitochondrial Gene Therapy Improves Respiration, Biogenesis, and Transcription in G11778A Leber's Hereditary Optic Neuropathy and T8993G Leigh's Syndrome Cells. Hum Gene Ther : (2012).Read more (PubMed: 22390282) »
- Tahbaz N et al. Role of polynucleotide kinase/phosphatase in mitochondrial DNA repair. Nucleic Acids Res : (2011). ICC/IF; Human.Read more (PubMed: 22210862) »
See all 5 publications for this product
Publishing research using ab110329? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"