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Mitofilin protein (Tagged-His Tag) (ab92008)

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Overview

  • Product nameMitofilin protein (Tagged-His Tag)
  • Protein descriptionRecombinant fragment, corresponding to amino acids 378-747 of Human Mitofilin (Isoform 3) with N terminal His tag; 370 amino acids, 47kDa (42.3kDa predicted from sequence). UniProt ID = Q16891.
  • Expression hostE. coli

    • Properties

  • Purification notesPurity level >85%
    This protein was expressed as an N-terminal His-tag fusion protein using Escherichia coli, and purified using Immobilized Metal Ion Affinity Chromatography. In some cases, smaller protein fragments may be present in addition to the intended expression product as a result of premature termination during translation in E. coli and subsequent co-purification via the His-tag. In some cases purified proteins run at a molecular weight different to the theoretically calculated molecular weight. This may be as a result of unequally distributed charges in the amino acid sequence. Alternatively, dimerisation of the expression product can occur under oxygen limitation during expression/cultivation.
  • FormLyophilised:Reconstitute with 59 µl aqua dest.
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 0.5% Trehalose, 6M Urea, 100mM Sodium phosphate, 10mM Sodium chloride, pH 4.5
  • Concentration information loading...
  • Additional notesProtein Identity confirmed by Mass Spectrometry (MS/MS) (acquired on initial reference batch).
  • Sequence notesVSDLADKLSTDDLNSLIAHAHRRIDQLNRELAEQKATEKQ HITLALEKQKLEEKRAFDS AVAKALEHHRSEIQAEQDR KIEEVRDAMENEMRTQLRRQAAAHTDHLRDVLRVQEQELK SEFEQNLSEKLSEQELQFRRLSQEQVDNFTLDINTAYA RLRGIEQAVQSHAVAEEEARK AHQLWLSVEALKYSMKT SSAETPTIPLGSAVEAIKANCSDNEFTQALTAAIPPESLT RG VYSEETLRARFYAVQKLARRVAMIDETRNSLYQYFL SYLQSLLLFPPQQLKPPPELCPE DINTFKLLSYASYCI EHGDLELAAKFVNQLKGESRRVAQDWLKEARMTLETKQIV EILT AYASAVGIGTTQVQPE
  • Research Areas

    • Applications

    Our Abpromise guarantee covers the use of ab92008 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    SDS-PAGE
    MS
  • Application notesMS: Use at an assay dependent dilution.
    SDS-PAGE: Use at an assay dependent dilution.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • Protein info

    • Alternative names
        Cell proliferation inducing protein 52Cell proliferation-inducing gene 4/52 proteinHMP
        IMMTIMMT_HUMANInner membrane protein mitochondrialInner mitochrondial membraneMINOS2Mitochondrial inner membrane organizing system 2Mitochondrial inner membrane proteinMitofilinMotor proteinp87p87/89p89pig4PIG52Proliferation-inducing gene 4
      see all
  • Cellular localizationMitochondrion inner membrane.

    • Target information above from: UniProt accession Q16891 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt

    Mitofilin protein (Tagged-His Tag) images

    • SDS-PAGE - Mitofilin protein (Tagged-His Tag) (ab92008)
      SDS-PAGE - Mitofilin protein (Tagged-His Tag) (ab92008)
      The image shows an electrophoretic assay performed using an Agilent 5100 ALP. In some images coloured control bands can be seen at 15 kDa (green) and/or 240 kDa (purple). The protein-specific band is blue.

    References for Mitofilin protein (Tagged-His Tag) (ab92008)

    ab92008 has not yet been referenced specifically in any publications.

    Publishing research using ab92008 ? Please let us know so that we can cite the reference in this datasheet

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