Overview

  • Product nameAnti-Mitofusin 1 antibodySee all Mitofusin 1 primary antibodies ...
  • Description
    Mouse monoclonal to Mitofusin 1
  • Tested applicationsWB, IHC-P, ICC/IF, Flow Cyt, IPmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human, Cynomolgus Monkey
  • Immunogen

    Recombinant full length protein, corresponding to amino acids 1-742 of Human Mitofusin 1

Properties

Applications

Our Abpromise guarantee covers the use of ab57602 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 84 kDa.
IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF Use a concentration of 5 µg/ml.
Flow Cyt Use 1µg for 106 cells.
IP Use at an assay dependent concentration.

Target

  • FunctionEssential transmembrane GTPase, which mediates mitochondrial fusion. Fusion of mitochondria occurs in many cell types and constitutes an important step in mitochondria morphology, which is balanced between fusion and fission. MFN1 acts independently of the cytoskeleton. Overexpression induces the formation of mitochondrial networks.
  • Tissue specificityUbiquitous. Expressed at slightly higher level in kidney and heart. Isoform 2 may be overexpressed in some tumors, such as lung cancers.
  • Sequence similaritiesBelongs to the mitofusin family.
  • Post-translational
    modifications
    Ubiquitinated by MARCH5.
  • Cellular localizationCytoplasm and Mitochondrion outer membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Fzo homolog antibody
    • Hfzo1 antibody
    • Hfzo2 antibody
    • MFN 1 antibody
    • Mfn1 antibody
    • MFN1_HUMAN antibody
    • Mitochondrial transmembrane GTPase Fzo 1 antibody
    • Mitochondrial transmembrane GTPase FZO 2 antibody
    • Mitochondrial transmembrane GTPase FZO1B antibody
    • Mitofusin 1 antibody
    • Mitofusin-1 antibody
    • Mitofusin1 antibody
    • Putative transmembrane GTPase antibody
    • Transmembrane GTPase MFN1 antibody
    see all

Anti-Mitofusin 1 antibody images

  • All lanes : Anti-Mitofusin 1 antibody (ab57602) at 1/1000 dilution

    Lane 1 : Cynomolgus Monkey peripheral blood mononuclear whole cell lysate
    Lane 2 : Cynomolgus Monkey peripheral blood mononuclear whole cell lysate
    Lane 3 : Cynomolgus Monkey peripheral blood mononuclear whole cell lysate
    Lane 4 : Cynomolgus Monkey peripheral blood mononuclear whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    HRP-conjugated Goat anti-mouse IgG polyclonal at 1/2000 dilution

    Performed under reducing conditions.

    Predicted band size : 84 kDa
    Observed band size : 75 kDa (why is the actual band size different from the predicted?)


    Exposure time : 30 seconds

    This image is courtesy of an Abreview submitted by Daniel Tyrrell

    Isolated PBMC whole cell lysates boiled for 5 minutes and loaded 20µg/well. 4 monkeys are represented in the image.

    See Abreview

  • ab57602 staining Mitofusin 1 in the H9C2 cell line from Rat cardiomyocytes by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with Triton X-100 1% and blocked with 5% serum for 60 minutes at 21°C. Samples were incubated with primary antibody (1/200 in PBS + 5% Goat serum) for 2 hours at 21°C. An Alexa Fluor®488-conjugated Goat anti-mouse IgG polyclonal(1/400) was used as the secondary antibody.

    See Abreview



  • Predicted band size : 84 kDa
    Mitofusin 1 antibody (ab57602) at 1ug/lane + HeLa cell lysate at 25ug/lane.
  • ICC/IF image of ab57602 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab57602, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • IHC image of ab57602 staining in human normal kidney formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab57602, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • Overlay histogram showing HEK293 cells stained with ab57602 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab57602, 1µg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

  • Mitofusin 1 was immunoprecipitated using 0.5mg Hela whole cell extract, 10ug of Mouse monoclonal to Mitofusin 1 and 50µl of protein G magnetic beads (lane 1). The antibody was incubated with the Protein G beads for 10 min under agitation. No antibody was added to the control lane 2 and no extract or antibody was added to contol lane 3. Hela whole cell extract diluted in RIPA buffer was added to each sample and incubated for 10min under agitation. Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab57602.
    Secondary: Goat polyclonal to mouse IgG light chain specific (HRP) at 1/5000 dilution.
    Bands: 84kDa:Mitofusin 1; 60kDa bead background: non specific - 48kDa: We are unsure as to the identity of this extra band.

References for Anti-Mitofusin 1 antibody (ab57602)

This product has been referenced in:
  • MacVicar TD & Lane JD Impaired OMA1-dependent cleavage of OPA1 and reduced DRP1 fission activity combine to prevent mitophagy in cells that are dependent on oxidative phosphorylation. J Cell Sci 127:2313-25 (2014). Read more (PubMed: 24634514) »
  • Yun J  et al. MUL1 acts in parallel to the PINK1/parkin pathway in regulating mitofusin and compensates for loss of PINK1/parkin. Elife (Cambridge) 3:e01958 (2014). WB ; Human . Read more (PubMed: 24898855) »

See all 7 Publications for this product

Product Wall

Application Western blot
Loading amount 40 µg
Gel Running Conditions Reduced Denaturing (10% SDS PAGE)
Sample Mouse Tissue lysate - whole (Liver)
Specification Liver
Blocking step Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Jun 09 2014

Application Western blot
Loading amount 20 µg
Gel Running Conditions Reduced Denaturing (12% Bis-Tris)
Sample Cynomolgus Monkey Cell lysate - whole cell (Peripheral Blood Mononuclear Cell)
Specification Peripheral Blood Mononuclear Cell
Blocking step Milk as blocking agent for 18 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Username

Mr. Daniel Tyrrell

Verified customer

Submitted Oct 18 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: rt°C
Sample Human Cell (hek293)
Specification hek293
Permeabilization Yes - 1% triton x-100
Fixative Paraformaldehyde
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Submitted Jul 05 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: rt°C
Sample Mouse Cell (MEF)
Specification MEF
Permeabilization Yes - 1% triton x-100
Fixative Paraformaldehyde
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Submitted Jul 04 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Rat Cell (h9c2)
Specification h9c2
Fixative Paraformaldehyde
Permeabilization Yes - 1% triton x100
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: rt°C
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Submitted Apr 03 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Rat Cell lysate - whole cell (h9c2 cell)
Loading amount 30 µg
Specification h9c2 cell
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: rt°C
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Submitted Apr 01 2013

Application Western blot
Sample Drosophila C Virus Tissue lysate - whole (fly heart tube)
Loading amount 10 µg
Specification fly heart tube
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: rt°C
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Submitted Feb 19 2013

I believe there has been a misunderstanding here.

We try to provide all the information which we have regarding any product we carry and provide the most thorough data we can on our datasheets as you have undoubtably seen when using our Mito...

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Thank you very much for your interest in our Mitofusin 1 antibodies.

Unfortunately the exact sequences of many of the immunogens used to create our proprietary. I have BLAST-ed the human and mouse Mitofusin 1 sequences and found that the ide...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (HEK 293T)
Loading amount 20 µg
Specification HEK 293T
Gel Running Conditions Reduced Denaturing (10% Bis Tris)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Dec 01 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"