Anti-Mitofusin 1 antibody (ab57602)
- Product nameAnti-Mitofusin 1 antibodySee all Mitofusin 1 primary antibodies ...
- DescriptionMouse monoclonal to Mitofusin 1
- Tested applicationsWB, IHC-P, ICC/IF, Flow Cyt, IP more details
- Species reactivityReacts with: Mouse, Rat, Human, Cynomolgus Monkey
Recombinant full length protein, corresponding to amino acids 1-742 of Human Mitofusin 1
- Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
- Storage bufferPreservative: None
PBS, pH 7.2
- Concentration information loading...
- PurityProtein G purified
- Clonality Monoclonal
- Light chain typekappa
Our Abpromise guarantee covers the use of ab57602 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||WB: Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 84 kDa.|
|IHC-P||IHC-P: Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|ICC/IF||ICC/IF: Use a concentration of 5 µg/ml.|
|Flow Cyt||Flow Cyt: Use 1µg for 106 cells.|
|IP||IP: Use at an assay dependent concentration.|
- FunctionEssential transmembrane GTPase, which mediates mitochondrial fusion. Fusion of mitochondria occurs in many cell types and constitutes an important step in mitochondria morphology, which is balanced between fusion and fission. MFN1 acts independently of the cytoskeleton. Overexpression induces the formation of mitochondrial networks.
- Tissue specificityUbiquitous. Expressed at slightly higher level in kidney and heart. Isoform 2 may be overexpressed in some tumors, such as lung cancers.
- Sequence similaritiesBelongs to the mitofusin family.
modificationsUbiquitinated by MARCH5.
- Cellular localizationCytoplasm and Mitochondrion outer membrane.
- Fzo homolog antibody
- Hfzo1 antibody
- Hfzo2 antibody
- MFN 1 antibody
- Mfn1 antibody
- MFN1_HUMAN antibody
- Mitochondrial transmembrane GTPase Fzo 1 antibody
- Mitochondrial transmembrane GTPase FZO 2 antibody
- Mitochondrial transmembrane GTPase FZO1B antibody
- Mitofusin 1 antibody
- Mitofusin-1 antibody
- Mitofusin1 antibody
- Putative transmembrane GTPase antibody
- Transmembrane GTPase MFN1 antibody
Anti-Mitofusin 1 antibody images
All lanes : Anti-Mitofusin 1 antibody (ab57602) at 1/1000 dilution
Lane 1 : Cynomolgus Monkey peripheral blood mononuclear whole cell lysate
Lane 2 : Cynomolgus Monkey peripheral blood mononuclear whole cell lysate
Lane 3 : Cynomolgus Monkey peripheral blood mononuclear whole cell lysate
Lane 4 : Cynomolgus Monkey peripheral blood mononuclear whole cell lysate
Lysates/proteins at 20 µg per lane.
HRP-conjugated Goat anti-mouse IgG polyclonal at 1/2000 dilution
Performed under reducing conditions.
Predicted band size : 84 kDa
Observed band size : 75 kDa (why is the actual band size different from the predicted?)
Exposure time : 30 seconds
This image is courtesy of an Abreview submitted by Daniel Tyrrell
Isolated PBMC whole cell lysates boiled for 5 minutes and loaded 20µg/well. 4 monkeys are represented in the image.
ab57602 staining Mitofusin 1 in the H9C2 cell line from Rat cardiomyocytes by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with Triton X-100 1% and blocked with 5% serum for 60 minutes at 21°C. Samples were incubated with primary antibody (1/200 in PBS + 5% Goat serum) for 2 hours at 21°C. An Alexa Fluor®488-conjugated Goat anti-mouse IgG polyclonal(1/400) was used as the secondary antibody.
Predicted band size : 84 kDa
Mitofusin 1 antibody (ab57602) at 1ug/lane + HeLa cell lysate at 25ug/lane.
ICC/IF image of ab57602 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab57602, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
IHC image of ab57602 staining in human normal kidney formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab57602, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
Overlay histogram showing HEK293 cells stained with ab57602 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab57602, 1µg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
Mitofusin 1 was immunoprecipitated using 0.5mg Hela whole cell extract, 10ug of Mouse monoclonal to Mitofusin 1 and 50µl of protein G magnetic beads (lane 1). The antibody was incubated with the Protein G beads for 10 min under agitation. No antibody was added to the control lane 2 and no extract or antibody was added to contol lane 3. Hela whole cell extract diluted in RIPA buffer was added to each sample and incubated for 10min under agitation. Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab57602.
Secondary: Goat polyclonal to mouse IgG light chain specific (HRP) at 1/5000 dilution.
Bands: 84kDa:Mitofusin 1; 60kDa bead background: non specific - 48kDa: We are unsure as to the identity of this extra band.
References for Anti-Mitofusin 1 antibody (ab57602)
This product has been referenced in:
- Tasseva G et al. Phosphatidylethanolamine deficiency in Mammalian mitochondria impairs oxidative phosphorylation and alters mitochondrial morphology. J Biol Chem 288:4158-73 (2013). WB . Read more (PubMed: 23250747) »
- Mancini C et al. Genome-wide expression profiling and functional characterization of SCA28 lymphoblastoid cell lines reveal impairment in cell growth and activation of apoptotic pathways. BMC Med Genomics 6:22 (2013). WB ; Human . Read more (PubMed: 23777634) »