![Western blot - Moesin antibody [EP1863Y] (ab52490) image](#ab52490_1.jpg)
![Immunohistochemistry (Paraffin-embedded sections) - Moesin antibody [EP1863Y] (ab52490) image](#ab52490_2.jpg)
![Flow Cytometry - Moesin antibody [EP1863Y] (ab52490) image](#Moesin-Primary-antibodies-ab52490-2.jpg)
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Product name
Anti-Moesin antibody [EP1863Y]
See all Moesin products (12) ...
Description
Rabbit monoclonal [EP1863Y] to Moesin
Specificity
This antibody reacts with Moesin
Tested applications
IHC-FoFr, WB, IP, ICC, Flow Cyt, IHC-Pmore details
Cross reactivity
Reacts with
Mouse, Rat, Human
Immunogen
Synthetic peptide corresponding to residues near the C terminus of human Moesin
Positive control
Human tonsil tissue and Hela cell lysate
General notes
Produced under U.S. Patent No. 5,675,063.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Purity
Tissue culture supernatant
Clonality
Monoclonal
Clone number
EP1863Y
Isotype
IgG
Research areas
Signal Transduction >> Cytoskeleton / ECM >> Cytoskeleton >> Microfilaments >> Talin / Moesin
Applications
Show applications key
Our Abpromise guarantee covers the use of ab52490 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-FoFr: Use at an assay dependent dilution.
WB: 1/1000 - 1/10000. Detects a band of approximately 68 kDa (predicted molecular weight: 68 kDa).
IP
IP: 1/70.IP: 1/70.
ICC
ICC: 1/100 - 1/250.ICC: 1/100 - 1/250.
Flow Cyt: 1/100.
IHC-P: 1/100 - 1/250.
Target
Function
Probably involved in connections of major cytoskeletal structures to the plasma membrane.
Tissue specificity
In all tissues and cultured cells studied.
Sequence similarities
Contains 1 FERM domain.
Post-translational
modifications
Phosphorylation on Thr-558 is crucial for the formation of microvilli-like structures.
Cellular localization
Cell membrane. Cytoplasm > cytoskeleton. Apical cell membrane. Cell projection > microvillus membrane. Phosphorylated form is enriched in microvilli-like structures at apical membrane (By similarity). Increased cell membrane localization of both phosphorylated and non-phosphorylated forms seen after thrombin treatment.
Target information above from: UniProt accessionP26038
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Membrane organizing extension spike protein antibody
- Membrane-organizing extension spike protein antibody
- MOES_HUMAN antibody
see all
Database links
- Entrez Gene: 4478 Human
- Entrez Gene: 17698 Mouse
- Entrez Gene: 81521 Rat
- Omim: 309845 Human
- SwissProt: P26038 Human
- SwissProt: P26041 Mouse
- SwissProt: O35763 Rat
- Unigene: 87752 Human
see all
Anti-Moesin antibody [EP1863Y] images:
Western blot - Moesin antibody [EP1863Y] (ab52490)
![Western blot - Moesin antibody [EP1863Y] (ab52490)](/ps/datasheet/Images/52/ab52490/ab52490_1.jpg)
Anti-Moesin antibody [EP1863Y] (ab52490) at 1/100000 dilution + Hela cell lysate at 10 µg
Secondary
Goat anti-rabbit HRP labeled at 1/2000 dilution
Predicted band size : 68 kDa
Observed band size : 68 kDa
Immunohistochemistry (Paraffin-embedded sections) - Moesin antibody [EP1863Y] (ab52490)
![Immunohistochemistry (Paraffin-embedded sections) - Moesin antibody [EP1863Y] (ab52490)](/ps/datasheet/Images/52/ab52490/ab52490_2.jpg)
Immunohistochemical staining of paraffin-embedded human tonsils using ab52490 at a 1/100 dilution.
Flow Cytometry - Moesin antibody [EP1863Y] (ab52490)
![Flow Cytometry - Moesin antibody [EP1863Y] (ab52490)](/ps/datasheet/images/52/ab52490/Moesin-Primary-antibodies-ab52490-2.jpg)
Overlay histogram showing HeLa cells stained with ab52490 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab52490, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1μg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
References for Anti-Moesin antibody [EP1863Y] (ab52490)
This product has been referenced in:
- Strilic B et al. The molecular basis of vascular lumen formation in the developing mouse aorta. Dev Cell 17:505-15 (2009). IHC-FoFr; Mouse.Read more (PubMed: 19853564) »
See 1 publication for this product
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"