Loading...
You have changed your country from
to
. Please be aware that this will change the currency in the purchasing process.
Reassurance, Refunds & Replacements
If your product does not perform as described on this datasheet, we will refund or replace your product...
Read our guarantee »Overview
Product name
Monoamine oxidase B (MAOB) Specific Activity Assay Kit
Tests
1 x 96 well plate
Sample type
Cell culture extracts
Assay type
Enzyme activity
Cross reactivity
Reacts with
Human
Product overview
ab109912 (MS747) is a novel assay that uses a high affinity monoclonal capture antibody to selectively isolate MAOB from all other peroxidases and oxidases (including MAOA) in a tissue or cultured cell sample. After isolation and subsequent measurement of the enzyme's functional activity, the quantity of isolated MAOB is measured in the same well by adding a second monoclonal detector antibody, which is quantified using a colorimetric label (HRP). Both reactions take place in time-dependent manners proportional to the amount of enzyme captured in each well. By combining activity and quantity measurements, the enzyme's relative specific activity can be determined. Specific activity is useful for measuring up or down regulation of activity by site-specific modification or damage, and in response to specific inhibitors.
Notes
Store Fluorophore and benzylamine at -80°C. Store all other components store at 4°C.
Tested applications
Sandwich ELISAmore details
Properties
Storage instructions
Please see Notes section
| Components | 1 x 96 tests |
|---|---|
| 100X Benzylamine Substrate | 1 x 0.25ml |
| 100X Detector Antibody | 1 x 0.125ml |
| 100X HRP Label | 1 x 0.125ml |
| 10X Blocking Solution | 1 x 10ml |
| 12-channel Reagent Reservoir | 1 unit |
| 1X Development Solution | 1 x 12ml |
| 20X Buffer | 1 x 25ml |
| 500X Fluorophore | 1 x 50µl |
| 500X Peroxidase | 1 x 50µl |
| 96-well Microplate (12 strips) | 1 unit |
| Extraction Buffer | 1 x 15ml |
Research areas
Metabolism >> Pathways and Processes >> Metabolic signaling pathways >> Drug metabolism
Metabolism >> Pathways and Processes >> Mitochondrial Metabolism >> Mitochondrial markers
Kits/ Lysates/ Other >> Kits >> Cell Metabolism Kits >> Other Metabolism Assay
Signal Transduction >> Metabolism >> Drug metabolism
Signal Transduction >> Metabolism >> Mitochondrial
Neuroscience >> Neurology process >> Neurodegenerative disease >> Parkinson's disease >> Other
Neuroscience >> Neurotransmitter >> Biogenic Amines >> Dopamine
Applications
Show applications key
Our Abpromise guarantee covers the use of ab109912 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
sELISA
Monoamine oxidase B (MAOB) Specific Activity Assay Kit images:
Sandwich ELISA - Monoamine oxidase B (MAOB) Specific Activity Assay Kit (ab109912)
Abcam's protein quantity microplate assays use the well-established sandwich ELISA format, whereby capture and detector antibodies are used to immobilize and then quantify a target protein or enzyme. All of our microplate assays utilize our highly-validated immunocapture antibodies, which are able to capture large, multi-subunit enzyme complexes in their fully intact state. Capture antibodies are pre-coated in the wells of premium Nunc MaxiSorp™ modular microplates, which can be broken into 8-well strips. After the target has been immobilized in the well, a second monoclonal antibody, against a different epitope on the target, is added to the well. This detector antibody is either directly labeled with biotin, or a biotin-labeled goat anti-mouse secondary is added. Substrate plus HRP or AP conjugated to streptavidin provide a colorimetric signal that is readable by any plate readers capable of standard ELISA absorbance measurements.
- Monoamine oxidase B (MAOB) Specific Activity Assay Kit (ab109912)
Figure 1. With a HepG2 cell lysate MAOB activity was clearly measurable in the 16-1000 µg/ mL range and quantity in the range 1-1000 µg/mL. The MAOB specific inhibitor pargyline inhibited activity 90% while not affecting quantity.
- Monoamine oxidase B (MAOB) Specific Activity Assay Kit (ab109912)
Figure 2. MAOB is selectively inhibited by selegiline and pargyline, but not clorgyline. In this example, raw data was exported to Graph Pad Prism for 4-parameter fit analysis and IC50 determination.
Protocols
References for Monoamine oxidase B (MAOB) Specific Activity Assay Kit (ab109912)
ab109912 has not yet been referenced specifically in any publications.
Publishing research using ab109912? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"