MitoSciences (MS747)

Monoamine oxidase B (MAOB) Specific Activity Assay Kit (ab109912)

Overview

  • Product nameMonoamine oxidase B (MAOB) Specific Activity Assay Kit
    See all Monoamine Oxidase B kits
  • Detection methodColorimetric
  • Tests
    1 x 96 well plate
  • Sample type
    Cell culture extracts
  • Assay typeEnzyme activity
  • Assay durationMultiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    ab109912 (MS747) is a novel assay that uses a high affinity monoclonal capture antibody to selectively isolate MAOB from all other peroxidases and oxidases (including MAOA) in a tissue or cultured cell sample. After isolation and subsequent measurement of the enzyme's functional activity, the quantity of isolated MAOB is measured in the same well by adding a second monoclonal detector antibody, which is quantified using a colorimetric label (HRP). Both reactions take place in time-dependent manners proportional to the amount of enzyme captured in each well. By combining activity and quantity measurements, the enzyme's relative specific activity can be determined. Specific activity is useful for measuring up or down regulation of activity by site-specific modification or damage, and in response to specific inhibitors.

  • Notes

    Store Fluorophore and benzylamine at -80°C. Store all other components store at 4°C.

  • Tested applicationsSandwich ELISAmore details
  • PlatformMicroplate

Properties

  • Alternative names
    • Amine oxidase [flavin-containing] B
    • MAO-B
    • MAOB
    • Monoamine oxidase type B

Applications

Our Abpromise guarantee covers the use of ab109912 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent dilution.

Monoamine oxidase B (MAOB) Specific Activity Assay Kit images

  • Abcam's protein quantity microplate assays use the well-established sandwich ELISA format, whereby capture and detector antibodies are used to immobilize and then quantify a target protein or enzyme. All of our microplate assays utilize our highly-validated immunocapture antibodies, which are able to capture large, multi-subunit enzyme complexes in their fully intact state. Capture antibodies are pre-coated in the wells of premium Nunc MaxiSorp™ modular microplates, which can be broken into 8-well strips. After the target has been immobilized in the well, a second monoclonal antibody, against a different epitope on the target, is added to the well. This detector antibody is either directly labeled with biotin, or a biotin-labeled goat anti-mouse secondary is added. Substrate plus HRP or AP conjugated to streptavidin provide a colorimetric signal that is readable by any plate readers capable of standard ELISA absorbance measurements.
  • Figure 1. With a HepG2 cell lysate MAOB activity was clearly measurable in the 16-1000 µg/ mL range and quantity in the range 1-1000 µg/mL. The MAOB specific inhibitor pargyline inhibited activity 90% while not affecting quantity.
  • Figure 2. MAOB is selectively inhibited by selegiline and pargyline, but not clorgyline. In this example, raw data was exported to Graph Pad Prism for 4-parameter fit analysis and IC50 determination.

Protocols

References for Monoamine oxidase B (MAOB) Specific Activity Assay Kit (ab109912)

ab109912 has not yet been referenced specifically in any publications.

Product Wall

Thank you for your response.

It is important to emphasize that as the datasheet suggests this kit has been designed and tested on cell culture extracts but not specifically on serum samples. As long as MAOB (in serum) is detectable, you could...

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Thank you for contacting us.
This kit is intended for use in measuring MAOB in cell culture samples. This assay is an immunoassay, a recombinant or purified standard would not be necessarily comparable to a native sample for activity so we don't o...

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Thank you for your enquiry.

The antibodies used in this kit were specially made for the kit and have never been released for sale as a standalone product. Therefore, there was no need to create a clone number for them and the information you...

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Thank you for your enquiry and your interest in our products.

The Monoamine oxidase B (MAOB) Specific Activity Assay Kit (ab109912) has been designed for cell culture extracts.

We would recommend using this kit in ‘profiling&r...

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Thank you for your inquiry.

1. I can confirm that this assay does not deliver the result asa quantitative amount of MAOB. This kit can be used to quantitatively measure the different between two samples.

For example. Sample 1 is unt...

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Thank you for contacting Abcam. Based on the sequence homology between human and dog (97%), there is a high probability that kit would work in samples from dog but we cannot guarantee that it. If there is anything else I can help you wi...

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Thank you for contacting us! This assay is an immunoassay, a recombinant or purified standard would not be necessarily comparable to a native sample for activity so we don't offer it. For example an MAOB recombinant protein is available from Abcam a...

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Thank you for your reply. I forwarded your question to the lab at Mitosciences, and they are not aware of a source of the active MAOB enzyme so they have not tested the kit with a recombinant standard. They recommend generating a standard curve wit...

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Thank you for your phone call today. The activity and quantity curves in the ab109912 protocol were generated using HepG2 cell lysate, so it's not really a typical standard. For the curve with the triangle icon, the HepG2 cells were treated with par...

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Thank you for your inquiry. These antibodies were generated using the shotgun immunization approach so we do not know the exact epitope. There was no peptide sequence used to immunize so unfortunately I cannot BLAST an immunogen sequence with pig. ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"