Anti-Monocyte + Macrophage antibody [MOMA-2] (ab33451)

  Immunohistochemistry (PFA perfusion fixed frozen sections) - Monocyte + Macrophage antibody [MOMA-2] (ab33451)

ab33451 staining mouse brain tissue sections by IHC-FoFr.  Sections were PFA fixed and blocked with 0.5% TNB for 30 minutes at 25°C.  The primary antibody was diluted 1/50 and incubated with the sample for 18 hours at 4°C.  A HRP conjugated goat anti-rat antibody, diluted 1/500, was used as the secondary. 

This antibody labels perivascular (left) and subpial (right) macs in normal brain tissue.

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  Immunohistochemistry (Frozen sections) - Monocyte + Macrophage antibody [MOMA-2] (ab33451)

ab33451 staining Monocyte + Macrophage in mouse suprarenal aorta sections by Immunohistonchemistry (Frozen sections).

Serial cryostat sections 7 µm thick were cut from suprarenal aortas with aneurysms prior to staining for inflammatory cells. Serial frozen sections were air-dried, fixed in acetone for 10 minutes at -20°C, air dried and rehydrated with PBS before being incubated in 3% H2O2/0.1% sodium azide/PBS to block endogenous peroxidase. For macrophage detection, sections were blocked in 2% normal goat serum in PBS followed by staining using ab33451. Slides were incubated in the peroxidase substrate 3, 3'-diamminobenzidine, counterstained in Mayer's Haematoxylin, dehydrated, cleared in xylene and mounted in Depex mounting medium.

Image from Rush C et al, BMC Genomics. 2009 Jul 6;10:298, Fig 3.

  Immunohistochemistry (Frozen sections) - Monocyte + Macrophage antibody [MOMA-2] (ab33451)

ab33451 staining Monocyte + Macrophage in Mouse aorta tissue by Immunohistochemistry (Frozen sections). The sections were fixed in acetone prior to blocking with 5% serum for 30 minutes at room temperature. The primary antibody was diluted 1/50 in PBS and incubated with the sample for 1 hour. A FITC-conjugated Rabbit anti-Rat polyclonal was used as the secondary antibody, diluted 1/400.
Endothelium is stained with Von Willebrand Factor (ab6994) and counterstained with DAPI

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