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The Mouse CD70 Enzyme-Linked Immunosorbent Assay (ELISA) kit (TNFSF7) (ab213854) is designed for the quantitative measurement of Mouse CD70 in cell culture supernatants, cell lysates, tissue homogenates, serum and plasma (heparin, EDTA).
The ELISA kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for CD70/TNFSF7 has been pre-coated onto 96-well plates. Standards (Expression system for standard: NSO; Immunogen sequence: S45-P195) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CD70/TNFSF7 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. HRP substrate TMB is used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Mouse CD70/TNFSF7 amount of sample captured in plate.
CD70 (Cluster of Differentiation 70), also known as TNFSF7 or CD27L, is a ligand for CD27. This gene is mapped to 19p13.3. CD70 is a surface antigen found on activated, but not resting, T and B lymphocytes. It has been found that CD70 induced proliferation of costimulated T cells and enhanced generation of cytolytic T cells. CD70 is homologous to the ligands of the TNF receptor family, including TNF-alpha, TNF-beta, and the CD40 ligand, showing 19 to 24% amino acid sequence identity in the extracellular region.
|Components||1 x 96 tests|
|ABC Diluent Buffer||1 x 12ml|
|Adhesive Plate Seal||4 units|
|Antibody Diluent Buffer||1 x 12ml|
|Anti-Mouse CD70 coated Microplate (12 x 8 wells)||1 unit|
|Avidin-Biotin-Peroxidase Complex (ABC)||1 x 130µl|
|Biotinylated anti-Mouse CD70 antibody||1 x 130µl|
|Lyophilized recombinant Mouse CD70 standard||2 vials|
|Sample Diluent Buffer||1 x 30ml|
|TMB Color Developing Agent||1 x 10ml|
|TMB Stop Solution||1 x 10ml|
Our Abpromise guarantee covers the use of ab213854 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Sandwich ELISA||Use at an assay dependent concentration.|
ab213854 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"