Overview

  • Product name
    Mouse CD70 ELISA Kit (TNFSF7)
  • Detection method
    Colorimetric
  • Precision
    Intra-assay
    Sample n Mean SD CV%
    1 16 94pg/ml 3.57 3.8%
    2 16 511pg/ml 26.06 5.1%
    3 16 1032pg/ml 44.38 4.3%
    Inter-assay
    Sample n Mean SD CV%
    1 24 148pg/ml 6.66 4.5%
    2 24 525pg/ml 32.55 6.2%
    3 24 1385pg/ml 77.56 5.6%
  • Sample type
    Cell culture supernatant, Serum, Cell Lysate, Heparin Plasma, EDTA Plasma, Tissue Homogenate
  • Assay type
    Sandwich (quantitative)
  • Sensitivity
    < 10 pg/ml
  • Range
    31.2 pg/ml - 2000 pg/ml
  • Assay time
    3h 30m
  • Assay duration
    Multiple steps standard assay
  • Species reactivity
    Reacts with: Mouse
  • Product overview

    The Mouse CD70 Enzyme-Linked Immunosorbent Assay (ELISA) kit (TNFSF7) (ab213854) is designed for the quantitative measurement of Mouse CD70 in cell culture supernatants, cell lysates, tissue homogenates, serum and plasma (heparin, EDTA). 


    The ELISA kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for CD70/TNFSF7 has been pre-coated onto 96-well plates. Standards (Expression system for standard: NSO; Immunogen sequence: S45-P195) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CD70/TNFSF7 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. HRP substrate TMB is used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Mouse CD70/TNFSF7 amount of sample captured in plate.

  • Notes

    CD70 (Cluster of Differentiation 70), also known as TNFSF7 or CD27L, is a ligand for CD27. This gene is mapped to 19p13.3. CD70 is a surface antigen found on activated, but not resting, T and B lymphocytes. It has been found that CD70 induced proliferation of costimulated T cells and enhanced generation of cytolytic T cells. CD70 is homologous to the ligands of the TNF receptor family, including TNF-alpha, TNF-beta, and the CD40 ligand, showing 19 to 24% amino acid sequence identity in the extracellular region.

  • Tested applications
    Suitable for: Sandwich ELISAmore details
  • Platform
    Pre-coated microplate (12 x 8 well strips)

Properties

Applications

Our Abpromise guarantee covers the use of ab213854 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • Mouse CD70 ELISA Kit (TNFSF7) (ab213854) Standard Curve.

Protocols

References

ab213854 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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