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|Sample type||Average %||Range|
|Serum||95||86% - 101%|
|Cell culture media||100||96% - 102%|
|Heparin Plasma||102||100% - 106%|
|EDTA Plasma||112||105% - 118%|
|Citrate Plasma||92||92% - 93%|
Abcam’s Chemerin mouse in vitro SimpleStep ELISA® (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of mouse Chemerin protein in serum, plasma, and cell culture supernatants.
The SimpleStep ELISA® employs an affinity tag labeled capture antibody and a reporter conjugated detector antibody which immunocapture the sample analyte in solution. This entire complex (capture antibody/analyte/detector antibody) is in turn immobilized via immunoaffinity of an anti-tag antibody coating the well. To perform the assay, samples or standards are added to the wells, followed by the antibody mix. After incubation, the wells are washed to remove unbound material. TMB substrate is added and during incubation is catalyzed by HRP, generating blue coloration. This reaction is then stopped by addition of Stop Solution completing any color change from blue to yellow. Signal is generated proportionally to the amount of bound analyte and the intensity is measured at 450 nm. Optionally, instead of the endpoint reading, development of TMB can be recorded kinetically at 600 nm.
Mouse Chemerin (also known as retinoic acid receptor responder protein 2, or Rarres2) is an adipocyte-secreted protein (adipokine) that regulates adipogenesis, metabolism and inflammation through activation of the chemokine-like receptor 1 (CMKLR1). Its other ligands include G protein-coupled receptor 1 (GPR1) and chemokine receptor-like 2 (CCRL2). Chemerin positively regulates adipocyte differentiation, modulates the expression of adipocyte genes involved in lipid and glucose metabolism and might play a role in angiogenesis, a process essential for the expansion of white adipose tissue. Furthermore, Chemerin also acts as a proinflammatory adipokine, causing an increase in secretion of proinflammatory and prodiabetic adipokines.
|Components||1 x 96 tests|
|10X Mouse Chemerin Detector Antibody||1 x 600µl|
|10X Wash Buffer PT (ab206977)||1 x 20ml|
|Antibody Diluent 4BI||1 x 6ml|
|Mouse Chemerin Capture Antibody(Lyophilized)||1 vial|
|Mouse Chemerin Lyophilized Recombinant Protein||2 vials|
|Plate Seals||1 unit|
|Sample Diluent NS||1 x 50ml|
|SimpleStep Pre-Coated 96-Well Microplate (ab206978)||1 unit|
|Stop Solution||1 x 12ml|
|TMB Substrate||1 x 12ml|
Our Abpromise guarantee covers the use of ab204520 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Sandwich ELISA||Use at an assay dependent concentration.|
Standard curve comparison between mouse Chemerin SimpleStep ELISA® kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows comparable sensitivity.
Background-subtracted data values (mean +/- SD) are graphed.
Native mouse Chemerin was measured in 0.5% mouse serum and 0.66% PHA + PMA stimulated L929 cultured media that were diluted in a 2-fold dilution series in Sample Diluent NS. Recombinant mouse Chemerin was spiked into 10% cell culture media and diluted in a 2-fold dilution series in Sample Diluent NS. The concentrations of mouse Chemerin were measured in duplicate and interpolated from the mouse Chemerin standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are graphed (mean +/- SD).
Native mouse Chemerin was measured in 0.33% mouse plasma citrate, 0.5% mouse plasma EDTA and 0.66% mouse plasma heparin that were diluted in a 2-fold dilution series in Sample Diluent NS. The concentrations of mouse Chemerin were measured in duplicate and interpolated from the mouse Chemerin standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are graphed (mean +/- SD).
During the exponential growth phase, L929 cells were treated for 72 hours in the presence and absence of 1.5% PHA and 10 ng/mL of PMA. Samples were diluted 1:300 in Sample Diluent NS. The concentrations of mouse Chemerin were interpolated from a standard curve diluted in Sample Diluent NS and corrected for sample dilution. The interpolated dilution factor corrected values are graphed (mean +/- SD). Note that media only gave an O.D. equivalent to background.
ab204520 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"