Mouse Embryonic Stem Cell Marker Panel (Oct4, Nanog, SOX2, SSEA1) (ab107156)

Overview

Mouse Embryonic Stem Cell Marker Panel (Oct4, Nanog, SOX2, SSEA1) images

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  Immunocytochemistry/ Immunofluorescence - Mouse Embryonic Stem Cell Marker Panel (Oct4, Nanog, SOX2, SSEA1) (ab107156)
  ICC/IF image of Oct4 (ab19857, green) and SSEA1 (ab16285, red) co-stained mouse embryonic stem cells. The cells were fixed in 4% paraformaldehyde and then permeabilized in blocking buffer (0.1% BSA, 1% serum, 0.1% triton in PBS) for 30 minutes at 25°C. The cells were then incubated with Oct4 (1:250) and SSEA1 (1:10) overnight at 4°C. The secondary antibodies, alexa fluor 488 goat anti rabbit IgG (green) and alexa fluor 647 goat anti mouse IgG (red), were both use at a 1:500 dilution for 1 hour. DAPI was used to stain the cell nuclei (blue).
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  Immunocytochemistry/ Immunofluorescence - Mouse Embryonic Stem Cell Marker Panel (Oct4, Nanog, SOX2, SSEA1) (ab107156)
  ab80892 staining Nanog (green) in mouse embryonic stem cells by immunocytochemistry/ immunofluorescence. Cells were paraformaldehyde fixed and permeabilized in blocking buffer (0.1% BSA, 1% serum, 0.1% triton in PBS) for 30 minutes at 25°C. The primary antibody was diluted 1:200 and incubated with the sample overnight at 4°C. An Alexa Fluor conjugated goat anti rabbit IgG antibody, diluted 1:500, was used as the secondary and incubated with samples for 1 hour at room temperature.
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  Immunocytochemistry/ Immunofluorescence - Mouse Embryonic Stem Cell Marker Panel (Oct4, Nanog, SOX2, SSEA1) (ab107156)
  ICC/IF image of Sox2 (ab97959, green) and SSEA1 (ab16285, red) co-stained mouse embryonic stem cells. The cells were fixed in paraformaldehyde and then permeabilized in blocking buffer (0.1% BSA, 1% serum, 0.1% triton in PBS) for 30 minutes at 25°C. The cells were then incubated with Sox2 (1:500) and SSEA1 (1:10) overnight at 4°C. The secondary antibodies, alexa fluor 488 goat anti rabbit IgG (green) and alexa fluor 647 goat anti mouse IgG (red), were both use at a 1:500 dilution for 1 hour at room temperature. DAPI was used to stain the cell nuclei (blue).
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  Immunocytochemistry/ Immunofluorescence - Mouse Embryonic Stem Cell Marker Panel (Oct4, Nanog, SOX2, SSEA1) (ab107156)
  ab16285 staining SSEA1 (red) in mouse embryonic stem cells by immunocytochemistry/ immunofluorescence. Cells were paraformaldehyde fixed and permeabilized in blocking buffer (0.1% BSA, 1% serum, 0.1% triton in PBS) for 30 minutes at 25°C. The primary antibody was diluted 1:10 and incubated with the sample overnight at 4°C. An Alexa Fluor 647 conjugated goat anti mouse IgG antibody, diluted 1:500, was used as the secondary and incubated with samples for 1 hour at room temperature.